Ethinylestradiol and Levonorgestrel as Active Agents in Normal Skin, and Pathological Conditions Induced by UVB Exposure: In Vitro and In Ovo Assessments
The link between melanoma development and the use of oral combined contraceptives is not fully elucidated, and the data concerning this issue are scarce and controversial. In the present study, we show that the components of oral contraceptives, ethinylestradiol (EE), levonorgestrel (LNG), and their...
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doaj-ea2fc46ff2cb4bbd9ac79882e8bc42a12020-11-25T01:56:30ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-11-011911360010.3390/ijms19113600ijms19113600Ethinylestradiol and Levonorgestrel as Active Agents in Normal Skin, and Pathological Conditions Induced by UVB Exposure: In Vitro and In Ovo AssessmentsDorina Coricovac0Claudia Farcas1Cristian Nica2Iulia Pinzaru3Sebastian Simu4Dana Stoian5Codruta Soica6Maria Proks7Stefana Avram8Dan Navolan9Catalin Dumitru10Ramona Amina Popovici11Cristina Adriana Dehelean12Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Dentistry, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaFaculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timișoara, RomaniaThe link between melanoma development and the use of oral combined contraceptives is not fully elucidated, and the data concerning this issue are scarce and controversial. In the present study, we show that the components of oral contraceptives, ethinylestradiol (EE), levonorgestrel (LNG), and their combination (EE + LNG) ± UVB (ultraviolet B radiation) induced differential effects on healthy (human keratinocytes, fibroblasts, and primary epidermal melanocytes, and murine epidermis cells) and melanoma cells (human—A375 and murine—B164A5), as follows: (i) at low doses (1 µM), the hormones were devoid of significant toxicity on healthy cells, but in melanoma cells, they triggered cell death via apoptosis; (ii) higher doses (10 µM) were associated with cytotoxicity in all cells, the most affected being the melanoma cells; (iii) UVB irradiation proved to be toxic for all types of cells; (iv) UVB irradiation + hormonal stimulation led to a synergistic cytotoxicity in the case of human melanoma cells—A375 and improved viability rates of healthy and B164A5 cells. A weak irritant potential exerted by EE and EE + LNG (10 µM) was assessed by the means of a chick chorioallantoic membrane assay. Further studies are required to elucidate the hormones’ cell type-dependent antimelanoma effect and the role played by melanin in this context.https://www.mdpi.com/1422-0067/19/11/3600ethinylestradiollevonorgestrelkeratinocytesfibroblastsmelanocytesmelanomaultraviolet radiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dorina Coricovac Claudia Farcas Cristian Nica Iulia Pinzaru Sebastian Simu Dana Stoian Codruta Soica Maria Proks Stefana Avram Dan Navolan Catalin Dumitru Ramona Amina Popovici Cristina Adriana Dehelean |
spellingShingle |
Dorina Coricovac Claudia Farcas Cristian Nica Iulia Pinzaru Sebastian Simu Dana Stoian Codruta Soica Maria Proks Stefana Avram Dan Navolan Catalin Dumitru Ramona Amina Popovici Cristina Adriana Dehelean Ethinylestradiol and Levonorgestrel as Active Agents in Normal Skin, and Pathological Conditions Induced by UVB Exposure: In Vitro and In Ovo Assessments International Journal of Molecular Sciences ethinylestradiol levonorgestrel keratinocytes fibroblasts melanocytes melanoma ultraviolet radiation |
author_facet |
Dorina Coricovac Claudia Farcas Cristian Nica Iulia Pinzaru Sebastian Simu Dana Stoian Codruta Soica Maria Proks Stefana Avram Dan Navolan Catalin Dumitru Ramona Amina Popovici Cristina Adriana Dehelean |
author_sort |
Dorina Coricovac |
title |
Ethinylestradiol and Levonorgestrel as Active Agents in Normal Skin, and Pathological Conditions Induced by UVB Exposure: In Vitro and In Ovo Assessments |
title_short |
Ethinylestradiol and Levonorgestrel as Active Agents in Normal Skin, and Pathological Conditions Induced by UVB Exposure: In Vitro and In Ovo Assessments |
title_full |
Ethinylestradiol and Levonorgestrel as Active Agents in Normal Skin, and Pathological Conditions Induced by UVB Exposure: In Vitro and In Ovo Assessments |
title_fullStr |
Ethinylestradiol and Levonorgestrel as Active Agents in Normal Skin, and Pathological Conditions Induced by UVB Exposure: In Vitro and In Ovo Assessments |
title_full_unstemmed |
Ethinylestradiol and Levonorgestrel as Active Agents in Normal Skin, and Pathological Conditions Induced by UVB Exposure: In Vitro and In Ovo Assessments |
title_sort |
ethinylestradiol and levonorgestrel as active agents in normal skin, and pathological conditions induced by uvb exposure: in vitro and in ovo assessments |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-11-01 |
description |
The link between melanoma development and the use of oral combined contraceptives is not fully elucidated, and the data concerning this issue are scarce and controversial. In the present study, we show that the components of oral contraceptives, ethinylestradiol (EE), levonorgestrel (LNG), and their combination (EE + LNG) ± UVB (ultraviolet B radiation) induced differential effects on healthy (human keratinocytes, fibroblasts, and primary epidermal melanocytes, and murine epidermis cells) and melanoma cells (human—A375 and murine—B164A5), as follows: (i) at low doses (1 µM), the hormones were devoid of significant toxicity on healthy cells, but in melanoma cells, they triggered cell death via apoptosis; (ii) higher doses (10 µM) were associated with cytotoxicity in all cells, the most affected being the melanoma cells; (iii) UVB irradiation proved to be toxic for all types of cells; (iv) UVB irradiation + hormonal stimulation led to a synergistic cytotoxicity in the case of human melanoma cells—A375 and improved viability rates of healthy and B164A5 cells. A weak irritant potential exerted by EE and EE + LNG (10 µM) was assessed by the means of a chick chorioallantoic membrane assay. Further studies are required to elucidate the hormones’ cell type-dependent antimelanoma effect and the role played by melanin in this context. |
topic |
ethinylestradiol levonorgestrel keratinocytes fibroblasts melanocytes melanoma ultraviolet radiation |
url |
https://www.mdpi.com/1422-0067/19/11/3600 |
work_keys_str_mv |
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