Evaluation of MiR-1908-3p as a novel serum biomarker for breast cancer and analysis its oncogenic function and target genes
Abstract Background Breast cancer is one of the most common tumors for women globally. Various miRNAs have been reported to play a crucial role in breast cancer, however the clinical significance of miR-1908-3p in breast cancer remains unclear. The present study aimed to explore the role of miR-1908...
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2020-07-01
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Online Access: | http://link.springer.com/article/10.1186/s12885-020-07125-4 |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Youzhi Zhu Qingshui Wang Yun Xia Xiaoxue Xiong Shuyun Weng Huizhen Ni Yan Ye Ling Chen Junyu Lin Yajuan Chen Haitao Niu Xiangjin Chen Yao Lin |
spellingShingle |
Youzhi Zhu Qingshui Wang Yun Xia Xiaoxue Xiong Shuyun Weng Huizhen Ni Yan Ye Ling Chen Junyu Lin Yajuan Chen Haitao Niu Xiangjin Chen Yao Lin Evaluation of MiR-1908-3p as a novel serum biomarker for breast cancer and analysis its oncogenic function and target genes BMC Cancer Breast cancer miR-1908-3p Proliferation Migration Invasion |
author_facet |
Youzhi Zhu Qingshui Wang Yun Xia Xiaoxue Xiong Shuyun Weng Huizhen Ni Yan Ye Ling Chen Junyu Lin Yajuan Chen Haitao Niu Xiangjin Chen Yao Lin |
author_sort |
Youzhi Zhu |
title |
Evaluation of MiR-1908-3p as a novel serum biomarker for breast cancer and analysis its oncogenic function and target genes |
title_short |
Evaluation of MiR-1908-3p as a novel serum biomarker for breast cancer and analysis its oncogenic function and target genes |
title_full |
Evaluation of MiR-1908-3p as a novel serum biomarker for breast cancer and analysis its oncogenic function and target genes |
title_fullStr |
Evaluation of MiR-1908-3p as a novel serum biomarker for breast cancer and analysis its oncogenic function and target genes |
title_full_unstemmed |
Evaluation of MiR-1908-3p as a novel serum biomarker for breast cancer and analysis its oncogenic function and target genes |
title_sort |
evaluation of mir-1908-3p as a novel serum biomarker for breast cancer and analysis its oncogenic function and target genes |
publisher |
BMC |
series |
BMC Cancer |
issn |
1471-2407 |
publishDate |
2020-07-01 |
description |
Abstract Background Breast cancer is one of the most common tumors for women globally. Various miRNAs have been reported to play a crucial role in breast cancer, however the clinical significance of miR-1908-3p in breast cancer remains unclear. The present study aimed to explore the role of miR-1908-3p in breast cancer. Methods The expression of miR-1908-3p was detected in 50 pairs of breast cancer tissues and adjacent normal tissues, 60 breast cancer patient serum and 60 healthy volunteer serum. The functional roles of miR-1908-3p in breast cancer cells such as proliferation, migration and invasion were evaluated using CCK8, SRB, wound healing and transwell chambers. In addition, bioinformatics tools were used to identify potential targets of miR-1908-3p. Results The results showed that the expression of miR-1908-3p were increased in breast cancer tissues and serum compared with normal breast tissues and serum of healthy volunteers respectively. Furthermore, the young breast cancer patients and HER2-positive patients had a higher level of tissues’ miR-1908-3p than elder breast cancer patients and HER2-negative patients, respectively. The young breast cancer patients had a higher level of serum miR-1908-3p than elder breast cancer patients, ROC analysis suggested that miR-1908-3p had the potential as a promising serum diagnostic biomarker of breast cancer. Up-regulation of miR-1908-3p promoted the cells proliferation, migration and invasion while knockdown of miR-1908-3p inhibited these processes in breast cancer cell MCF-7 and MDA-MB-231. The potential target genes of miR-1908-3p in breast cancer included ID4, LTBP4, GPM6B, RGMA, EFCAB1, ALX4, OSR1 and PPARA. Higher expression of these eight genes correlated with a better prognosis for breast cancer patients. Conclusions These results suggest that miR-1908-3p may exert its oncogenic functions via suppression of these eight genes in breast cancer. |
topic |
Breast cancer miR-1908-3p Proliferation Migration Invasion |
url |
http://link.springer.com/article/10.1186/s12885-020-07125-4 |
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doaj-ea33aa9bd0404985bb81fb7d134ea9fd2020-11-25T03:32:34ZengBMCBMC Cancer1471-24072020-07-0120111210.1186/s12885-020-07125-4Evaluation of MiR-1908-3p as a novel serum biomarker for breast cancer and analysis its oncogenic function and target genesYouzhi Zhu0Qingshui Wang1Yun Xia2Xiaoxue Xiong3Shuyun Weng4Huizhen Ni5Yan Ye6Ling Chen7Junyu Lin8Yajuan Chen9Haitao Niu10Xiangjin Chen11Yao Lin12Department of Thyroid and Breast Surgery, The First Affiliated Hospital of Fujian Medical UniversityKey Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityKey Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityKey Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityKey Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityKey Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityKey Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityDepartment of Thyroid and Breast Surgery, The First Affiliated Hospital of Fujian Medical UniversityDepartment of Thyroid and Breast Surgery, The First Affiliated Hospital of Fujian Medical UniversityKey Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityKey Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityDepartment of Thyroid and Breast Surgery, The First Affiliated Hospital of Fujian Medical UniversityKey Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityAbstract Background Breast cancer is one of the most common tumors for women globally. Various miRNAs have been reported to play a crucial role in breast cancer, however the clinical significance of miR-1908-3p in breast cancer remains unclear. The present study aimed to explore the role of miR-1908-3p in breast cancer. Methods The expression of miR-1908-3p was detected in 50 pairs of breast cancer tissues and adjacent normal tissues, 60 breast cancer patient serum and 60 healthy volunteer serum. The functional roles of miR-1908-3p in breast cancer cells such as proliferation, migration and invasion were evaluated using CCK8, SRB, wound healing and transwell chambers. In addition, bioinformatics tools were used to identify potential targets of miR-1908-3p. Results The results showed that the expression of miR-1908-3p were increased in breast cancer tissues and serum compared with normal breast tissues and serum of healthy volunteers respectively. Furthermore, the young breast cancer patients and HER2-positive patients had a higher level of tissues’ miR-1908-3p than elder breast cancer patients and HER2-negative patients, respectively. The young breast cancer patients had a higher level of serum miR-1908-3p than elder breast cancer patients, ROC analysis suggested that miR-1908-3p had the potential as a promising serum diagnostic biomarker of breast cancer. Up-regulation of miR-1908-3p promoted the cells proliferation, migration and invasion while knockdown of miR-1908-3p inhibited these processes in breast cancer cell MCF-7 and MDA-MB-231. The potential target genes of miR-1908-3p in breast cancer included ID4, LTBP4, GPM6B, RGMA, EFCAB1, ALX4, OSR1 and PPARA. Higher expression of these eight genes correlated with a better prognosis for breast cancer patients. Conclusions These results suggest that miR-1908-3p may exert its oncogenic functions via suppression of these eight genes in breast cancer.http://link.springer.com/article/10.1186/s12885-020-07125-4Breast cancermiR-1908-3pProliferationMigrationInvasion |