| Summary: | Background: Most of the techniques used to investigate choke vessels are indirect. The aim of the present study is to assess the effectiveness of percutaneous endoscopy in direct real-time visualization of choke vessels in rat perforator flap models. Methods: A classic perforator flap on the rat dorsum was designed (n = 12). An additional incision was made to place the percutaneous endoscope. Evans blue dye was injected from the common carotid artery to distinguish choke arteries from veins. Blood perfusion status was assessed using full-field laser perfusion imaging (FLPI) and the oxygen/carbon dioxide levels. Photographs of choke vessels were taken and compared at 1 h, 1 day, 4 days, and 7 days postoperation. The flap survival area was examined on day 7. Results: The average survival rate of perforator flaps was 70.1 ± 10.8%. The choke arteries but not choke veins were stained blue after injection of Evans blue dye. The choke arteries constricted instantly after surgery, dilated to a maximum diameter on postoperation day 4, and returned to the preoperation status on day 7. The choke veins dilated instantly after the operation, reached their largest diameters on postoperation day 4, and remained dilated on day 7. The behaviors of choke vessels were consistent with the FLPI results and oxygen/carbon dioxide statuses. Conclusion: Percutaneous endoscopy can provide direct real-time visualization of choke vessels in living rat perforator flap models and enable the identification of choke arteries and veins. This novel technique represents an ideal platform for investigating choke vessels in perforator flap models. Keywords: Percutaneous endoscopy, Choke vessel, Visualization, Perforator flap model
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