Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western China
Abstract Background Alveolar echinococcosis (AE) is a life-threatening human disease caused by Echinococcus multilocularis transmitted between rodents and dogs/foxes in the Northern Hemisphere. The study aims to identify the genetic variation of the parasite in AE patients from China. Methods E. mul...
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doaj-ea975e5e835d4aac9de1d28124dd10a12020-11-24T21:59:47ZengBMCParasites & Vectors1756-33052017-05-011011710.1186/s13071-017-2172-yGenetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western ChinaChuanchuan Wu0Wenbao Zhang1Bo Ran2Haining Fan3Hui Wang4Baoping Guo5Canlin Zhou6Yingmei Shao7Wei Zhang8Patrick Giraudoux9Jenny Knapp10Hao Wen11Ling Kuang12Jun Li13Clinic Medicine Research Institute, The First Teaching Hospital of Xinjiang Medical UniversityClinic Medicine Research Institute, The First Teaching Hospital of Xinjiang Medical UniversityCentre for Clinic Echinococcosis, The First Teaching Hospital of Xinjiang Medical UniversityDepartment of Hepatopancreatobiliary Surgery, Affiliated Hospital of Qinghai UniversityClinic Medicine Research Institute, The First Teaching Hospital of Xinjiang Medical UniversityClinic Medicine Research Institute, The First Teaching Hospital of Xinjiang Medical UniversityClinic Medicine Research Institute, The First Teaching Hospital of Xinjiang Medical UniversityCentre for Clinic Echinococcosis, The First Teaching Hospital of Xinjiang Medical UniversityClinic Medicine Research Institute, The First Teaching Hospital of Xinjiang Medical UniversityChrono-environmentLaboratory, University of Bourgogne Franche-ComtéChrono-environmentLaboratory, University of Bourgogne Franche-ComtéClinic Medicine Research Institute, The First Teaching Hospital of Xinjiang Medical UniversityCollage of Veterinary Medicine, Xinjiang Agriculture University UrumqiClinic Medicine Research Institute, The First Teaching Hospital of Xinjiang Medical UniversityAbstract Background Alveolar echinococcosis (AE) is a life-threatening human disease caused by Echinococcus multilocularis transmitted between rodents and dogs/foxes in the Northern Hemisphere. The study aims to identify the genetic variation of the parasite in AE patients from China. Methods E. multilocularis isolates were collected from wild small mammals (n = 6) and AE patients (n = 56) from western China. Genomic DNA was extracted from different tissue samples including paraffin tissue blocks, ethanol fixed tissues and frozen tissues surgically removed. Two mitochondrial gene fragments (526 bp for cob and 474 bp for nad2) of E. multilocularis were amplified and sequenced. Results The parasite fragment sequences of cob fragments from AE patients showed two haplotypes, and nad2 gene fragment sequences had four haplotypes. The gene sequences from Microtus sp. were 100% identical to the sequences of some isolates from AE patients. These haplotypes were distributed in both Qinghai and Xinjiang provinces. Alignment analysis with the sequences from the GenBank databases showed five genotypes including three Asian genotypes, one from Europe and one from North America. Conclusions Most AE patients harbored the Asian genotype 1 which may be an indication of its relative frequency in the definitive hosts and the environment or of its pathogenicity to humans, which calls for further research.http://link.springer.com/article/10.1186/s13071-017-2172-yEchinococcus multilocularisMitochondrial genescobnad2Genetic variationAlveolar echinococcosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chuanchuan Wu Wenbao Zhang Bo Ran Haining Fan Hui Wang Baoping Guo Canlin Zhou Yingmei Shao Wei Zhang Patrick Giraudoux Jenny Knapp Hao Wen Ling Kuang Jun Li |
spellingShingle |
Chuanchuan Wu Wenbao Zhang Bo Ran Haining Fan Hui Wang Baoping Guo Canlin Zhou Yingmei Shao Wei Zhang Patrick Giraudoux Jenny Knapp Hao Wen Ling Kuang Jun Li Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western China Parasites & Vectors Echinococcus multilocularis Mitochondrial genes cob nad2 Genetic variation Alveolar echinococcosis |
author_facet |
Chuanchuan Wu Wenbao Zhang Bo Ran Haining Fan Hui Wang Baoping Guo Canlin Zhou Yingmei Shao Wei Zhang Patrick Giraudoux Jenny Knapp Hao Wen Ling Kuang Jun Li |
author_sort |
Chuanchuan Wu |
title |
Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western China |
title_short |
Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western China |
title_full |
Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western China |
title_fullStr |
Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western China |
title_full_unstemmed |
Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western China |
title_sort |
genetic variation of mitochondrial genes among echinococcus multilocularis isolates collected in western china |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2017-05-01 |
description |
Abstract Background Alveolar echinococcosis (AE) is a life-threatening human disease caused by Echinococcus multilocularis transmitted between rodents and dogs/foxes in the Northern Hemisphere. The study aims to identify the genetic variation of the parasite in AE patients from China. Methods E. multilocularis isolates were collected from wild small mammals (n = 6) and AE patients (n = 56) from western China. Genomic DNA was extracted from different tissue samples including paraffin tissue blocks, ethanol fixed tissues and frozen tissues surgically removed. Two mitochondrial gene fragments (526 bp for cob and 474 bp for nad2) of E. multilocularis were amplified and sequenced. Results The parasite fragment sequences of cob fragments from AE patients showed two haplotypes, and nad2 gene fragment sequences had four haplotypes. The gene sequences from Microtus sp. were 100% identical to the sequences of some isolates from AE patients. These haplotypes were distributed in both Qinghai and Xinjiang provinces. Alignment analysis with the sequences from the GenBank databases showed five genotypes including three Asian genotypes, one from Europe and one from North America. Conclusions Most AE patients harbored the Asian genotype 1 which may be an indication of its relative frequency in the definitive hosts and the environment or of its pathogenicity to humans, which calls for further research. |
topic |
Echinococcus multilocularis Mitochondrial genes cob nad2 Genetic variation Alveolar echinococcosis |
url |
http://link.springer.com/article/10.1186/s13071-017-2172-y |
work_keys_str_mv |
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