Effects of Multivitamins and Known Teratogens on Chick Cardiomyocytes Micromass Culture Assay
Objective(s): This study aimed to find out whether the chick cardiomyocyte micromass (MM) system could be employed to predict the teratogenecity of common environmental factors. Different multivitamins and over the counter drugs were used in this study. Materials and Methods: White Leghorn 5-day...
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doaj-ea9a6e0ce6534d60983427e8fe5969842020-11-24T22:39:59ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences 2008-38662008-38742013-09-0116999610031681Effects of Multivitamins and Known Teratogens on Chick Cardiomyocytes Micromass Culture AssaySamreen Memon0Margaret Pratten1Department of Anatomy Liaquat University of Medical Sciences, PakistanSchool of Biomedical Sciences, University of Nottingham, UK Objective(s): This study aimed to find out whether the chick cardiomyocyte micromass (MM) system could be employed to predict the teratogenecity of common environmental factors. Different multivitamins and over the counter drugs were used in this study. Materials and Methods: White Leghorn 5-day-old embryo hearts were dissected and trypsinized to produce a cardiomyocyte cell suspension in Dulbecco's Modified Eagle's Medium. The cultures were incubated at 370C in 5% CO2 in air, and observations were made at 24, 48 and 144 hr, for the detection of cell beating. Cellular viability was assessed using the resazurin assay and cell protein content was assessed by the kenacid blue assay. It was observed that while not affecting total cell number folic acid, vitamin C, sodium fluoride and ginseng did not significantly reduced cell activity and beating. However cadmium chloride significantly reduced the beating, cell viability and cell protein content in micromass cultures. Results: The results demonstrate the potential of the chick cardiomyocyte MM culture assay to identify teratogens/embryotoxins that alter morphology and function, which may result in either teratogenic outcome or cytotoxicity. Conclusion: This could form part of a screen for developmental toxicity related to cardiac function http://ijbms.mums.ac.ir/pdf_1681_657534f64fca791725890a0047b61a61.htmlChick cardiomyocyte Environmental teratogens Micromass culture Multivitamins |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Samreen Memon Margaret Pratten |
spellingShingle |
Samreen Memon Margaret Pratten Effects of Multivitamins and Known Teratogens on Chick Cardiomyocytes Micromass Culture Assay Iranian Journal of Basic Medical Sciences Chick cardiomyocyte Environmental teratogens Micromass culture Multivitamins |
author_facet |
Samreen Memon Margaret Pratten |
author_sort |
Samreen Memon |
title |
Effects of Multivitamins and Known Teratogens on Chick Cardiomyocytes Micromass Culture Assay |
title_short |
Effects of Multivitamins and Known Teratogens on Chick Cardiomyocytes Micromass Culture Assay |
title_full |
Effects of Multivitamins and Known Teratogens on Chick Cardiomyocytes Micromass Culture Assay |
title_fullStr |
Effects of Multivitamins and Known Teratogens on Chick Cardiomyocytes Micromass Culture Assay |
title_full_unstemmed |
Effects of Multivitamins and Known Teratogens on Chick Cardiomyocytes Micromass Culture Assay |
title_sort |
effects of multivitamins and known teratogens on chick cardiomyocytes micromass culture assay |
publisher |
Mashhad University of Medical Sciences |
series |
Iranian Journal of Basic Medical Sciences |
issn |
2008-3866 2008-3874 |
publishDate |
2013-09-01 |
description |
Objective(s): This study aimed to find out whether the chick cardiomyocyte micromass (MM) system could be employed to predict the teratogenecity of common environmental factors. Different multivitamins and over the counter drugs were used in this study. Materials and Methods: White Leghorn 5-day-old embryo hearts were dissected and trypsinized to produce a cardiomyocyte cell suspension in Dulbecco's Modified Eagle's Medium. The cultures were incubated at 370C in 5% CO2 in air, and observations were made at 24, 48 and 144 hr, for the detection of cell beating. Cellular viability was assessed using the resazurin assay and cell protein content was assessed by the kenacid blue assay. It was observed that while not affecting total cell number folic acid, vitamin C, sodium fluoride and ginseng did not significantly reduced cell activity and beating. However cadmium chloride significantly reduced the beating, cell viability and cell protein content in micromass cultures. Results: The results demonstrate the potential of the chick cardiomyocyte MM culture assay to identify teratogens/embryotoxins that alter morphology and function, which may result in either teratogenic outcome or cytotoxicity. Conclusion: This could form part of a screen for developmental toxicity related to cardiac function |
topic |
Chick cardiomyocyte Environmental teratogens Micromass culture Multivitamins |
url |
http://ijbms.mums.ac.ir/pdf_1681_657534f64fca791725890a0047b61a61.html |
work_keys_str_mv |
AT samreenmemon effectsofmultivitaminsandknownteratogensonchickcardiomyocytesmicromasscultureassay AT margaretpratten effectsofmultivitaminsandknownteratogensonchickcardiomyocytesmicromasscultureassay |
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