Correlative x-ray phase-contrast tomography and histology of human brain tissue affected by Alzheimer’s disease
Alzheimer’s disease (AD) is a neurodegenerative disorder which is characterized by increasing dementia. It is accompanied by the development of extracellular β-amyloid plaques and neurofibrillary tangles in the gray matter of the brain. Histology is the gold standard for the visualization of this pa...
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doaj-eaa08536891547089259112f02f905852020-11-25T03:45:21ZengElsevierNeuroImage1095-95722020-04-01210116523Correlative x-ray phase-contrast tomography and histology of human brain tissue affected by Alzheimer’s diseaseMareike Töpperwien0Franziska van der Meer1Christine Stadelmann2Tim Salditt3Institute for X-Ray Physics, University of Göttingen, GermanyInstitute for Neuropathology, University Medical Center Göttingen, GermanyInstitute for Neuropathology, University Medical Center Göttingen, GermanyInstitute for X-Ray Physics, University of Göttingen, Germany; Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells”, University of Göttingen, Germany; Corresponding author. Institute for X-Ray Physics, University of Göttingen, GermanyAlzheimer’s disease (AD) is a neurodegenerative disorder which is characterized by increasing dementia. It is accompanied by the development of extracellular β-amyloid plaques and neurofibrillary tangles in the gray matter of the brain. Histology is the gold standard for the visualization of this pathology, but also has intrinsic shortcomings. Fully three-dimensional analysis and quantitative metrics of alterations in the tissue structure require a complementary approach. In this work we use x-ray phase-contrast tomography to obtain three-dimensional reconstructions of human hippocampal tissue affected by AD. Due to intrinsic electron density differences, tissue components and structures such as the granule cells of the dentate gyrus, blood vessels, or mineralized plaques can be identified and segmented in large volumes. Based on correlative histology, protein (tau, β-amyloid) and elemental content (iron, calcium) can be attributed to certain morphological features occurring in the entire volume. In the vicinity of senile plaques, an accumulation of microglia in combination with a loss of neuronal cells can be observed.http://www.sciencedirect.com/science/article/pii/S1053811920300100phase-contrast x-ray tomographySynchrotron-based x-ray imagingLaboratory-based x-ray imagingAlzheimer’s diseaseCorrelative imaging |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mareike Töpperwien Franziska van der Meer Christine Stadelmann Tim Salditt |
spellingShingle |
Mareike Töpperwien Franziska van der Meer Christine Stadelmann Tim Salditt Correlative x-ray phase-contrast tomography and histology of human brain tissue affected by Alzheimer’s disease NeuroImage phase-contrast x-ray tomography Synchrotron-based x-ray imaging Laboratory-based x-ray imaging Alzheimer’s disease Correlative imaging |
author_facet |
Mareike Töpperwien Franziska van der Meer Christine Stadelmann Tim Salditt |
author_sort |
Mareike Töpperwien |
title |
Correlative x-ray phase-contrast tomography and histology of human brain tissue affected by Alzheimer’s disease |
title_short |
Correlative x-ray phase-contrast tomography and histology of human brain tissue affected by Alzheimer’s disease |
title_full |
Correlative x-ray phase-contrast tomography and histology of human brain tissue affected by Alzheimer’s disease |
title_fullStr |
Correlative x-ray phase-contrast tomography and histology of human brain tissue affected by Alzheimer’s disease |
title_full_unstemmed |
Correlative x-ray phase-contrast tomography and histology of human brain tissue affected by Alzheimer’s disease |
title_sort |
correlative x-ray phase-contrast tomography and histology of human brain tissue affected by alzheimer’s disease |
publisher |
Elsevier |
series |
NeuroImage |
issn |
1095-9572 |
publishDate |
2020-04-01 |
description |
Alzheimer’s disease (AD) is a neurodegenerative disorder which is characterized by increasing dementia. It is accompanied by the development of extracellular β-amyloid plaques and neurofibrillary tangles in the gray matter of the brain. Histology is the gold standard for the visualization of this pathology, but also has intrinsic shortcomings. Fully three-dimensional analysis and quantitative metrics of alterations in the tissue structure require a complementary approach. In this work we use x-ray phase-contrast tomography to obtain three-dimensional reconstructions of human hippocampal tissue affected by AD. Due to intrinsic electron density differences, tissue components and structures such as the granule cells of the dentate gyrus, blood vessels, or mineralized plaques can be identified and segmented in large volumes. Based on correlative histology, protein (tau, β-amyloid) and elemental content (iron, calcium) can be attributed to certain morphological features occurring in the entire volume. In the vicinity of senile plaques, an accumulation of microglia in combination with a loss of neuronal cells can be observed. |
topic |
phase-contrast x-ray tomography Synchrotron-based x-ray imaging Laboratory-based x-ray imaging Alzheimer’s disease Correlative imaging |
url |
http://www.sciencedirect.com/science/article/pii/S1053811920300100 |
work_keys_str_mv |
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