Rapid screening of complex DNA samples by single-molecule amplification and sequencing.

Rapid screening of complex DNA samples by single-molecule amplification and sequencing.

Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram qu...

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Bibliographic Details
Main Authors: Jiaqi Huang, Zongli Zheng, Anders F Andersson, Lars Engstrand, Weimin Ye
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625543/?tool=EBI
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spelling doaj-eac2739637ef4a72a0cdcb799b0ebe402021-03-04T01:53:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0165e1972310.1371/journal.pone.0019723Rapid screening of complex DNA samples by single-molecule amplification and sequencing.Jiaqi HuangZongli ZhengAnders F AnderssonLars EngstrandWeimin YeMicrobial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625543/?tool=EBI
collection DOAJ
language English
format Article
sources DOAJ
author Jiaqi Huang
Zongli Zheng
Anders F Andersson
Lars Engstrand
Weimin Ye
spellingShingle Jiaqi Huang
Zongli Zheng
Anders F Andersson
Lars Engstrand
Weimin Ye
Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
PLoS ONE
author_facet Jiaqi Huang
Zongli Zheng
Anders F Andersson
Lars Engstrand
Weimin Ye
author_sort Jiaqi Huang
title Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
title_short Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
title_full Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
title_fullStr Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
title_full_unstemmed Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
title_sort rapid screening of complex dna samples by single-molecule amplification and sequencing.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2011-01-01
description Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.
url https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625543/?tool=EBI
work_keys_str_mv AT jiaqihuang rapidscreeningofcomplexdnasamplesbysinglemoleculeamplificationandsequencing
AT zonglizheng rapidscreeningofcomplexdnasamplesbysinglemoleculeamplificationandsequencing
AT andersfandersson rapidscreeningofcomplexdnasamplesbysinglemoleculeamplificationandsequencing
AT larsengstrand rapidscreeningofcomplexdnasamplesbysinglemoleculeamplificationandsequencing
AT weiminye rapidscreeningofcomplexdnasamplesbysinglemoleculeamplificationandsequencing
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