Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram qu...
Main Authors: | Jiaqi Huang, Zongli Zheng, Anders F Andersson, Lars Engstrand, Weimin Ye |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2011-01-01
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Series: | PLoS ONE |
Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625543/?tool=EBI |
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