L1CAM Is a Marker for Enriching Corticospinal Motor Neurons in the Developing Brain
The cerebral cortical tissue of murine embryo and pluripotent stem cell-derived neurons can survive in the adult brain and extend axons to the spinal cord. These features suggest that cell transplantation can be a strategy to reconstruct the corticospinal tract (CST). It is unknown, however, which c...
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doaj-ead32abef3ed487189c8e5969b70c4b62020-11-25T02:23:53ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022020-02-011410.3389/fncel.2020.00031477445L1CAM Is a Marker for Enriching Corticospinal Motor Neurons in the Developing BrainBumpei Samata0Rika Takaichi1Yuko Ishii2Kaori Fukushima3Harumi Nakagawa4Yuichi Ono5Jun Takahashi6Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University, Kyoto, JapanDepartment of Clinical Application, Center for iPS Cell Research and Application, Kyoto University, Kyoto, JapanDepartment of Clinical Application, Center for iPS Cell Research and Application, Kyoto University, Kyoto, JapanDepartment of Clinical Application, Center for iPS Cell Research and Application, Kyoto University, Kyoto, JapanDepartment of Developmental Neurobiology, KAN Research Institute Inc., Kobe, JapanDepartment of Developmental Neurobiology, KAN Research Institute Inc., Kobe, JapanDepartment of Clinical Application, Center for iPS Cell Research and Application, Kyoto University, Kyoto, JapanThe cerebral cortical tissue of murine embryo and pluripotent stem cell-derived neurons can survive in the adult brain and extend axons to the spinal cord. These features suggest that cell transplantation can be a strategy to reconstruct the corticospinal tract (CST). It is unknown, however, which cell population makes for safe and effective donor cells. To address this issue, we grafted the cerebral cortex of E14.5 mouse to the brain of adult mice and found that the cells in the graft extending axons along the CST expressed CTIP2. By using CTIP2:GFP knock-in mouse embryonic stem cells (mESCs), we identified L1CAM as a cell surface marker to enrich CTIP2+ cells. We sorted L1CAM+ cells from E14.5 mouse brain and confirmed that they extended a larger number of axons along the CST compared to L1CAM− cells. Our results suggest that sorting L1CAM+ cells from the embryonic cerebral cortex enriches subcortical projection neurons to reconstruct the CST.https://www.frontiersin.org/article/10.3389/fncel.2020.00031/fullL1 cell adhesion moleculecorticospinal motor neuronstransplantationcorticospinal tractcell sorting |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bumpei Samata Rika Takaichi Yuko Ishii Kaori Fukushima Harumi Nakagawa Yuichi Ono Jun Takahashi |
spellingShingle |
Bumpei Samata Rika Takaichi Yuko Ishii Kaori Fukushima Harumi Nakagawa Yuichi Ono Jun Takahashi L1CAM Is a Marker for Enriching Corticospinal Motor Neurons in the Developing Brain Frontiers in Cellular Neuroscience L1 cell adhesion molecule corticospinal motor neurons transplantation corticospinal tract cell sorting |
author_facet |
Bumpei Samata Rika Takaichi Yuko Ishii Kaori Fukushima Harumi Nakagawa Yuichi Ono Jun Takahashi |
author_sort |
Bumpei Samata |
title |
L1CAM Is a Marker for Enriching Corticospinal Motor Neurons in the Developing Brain |
title_short |
L1CAM Is a Marker for Enriching Corticospinal Motor Neurons in the Developing Brain |
title_full |
L1CAM Is a Marker for Enriching Corticospinal Motor Neurons in the Developing Brain |
title_fullStr |
L1CAM Is a Marker for Enriching Corticospinal Motor Neurons in the Developing Brain |
title_full_unstemmed |
L1CAM Is a Marker for Enriching Corticospinal Motor Neurons in the Developing Brain |
title_sort |
l1cam is a marker for enriching corticospinal motor neurons in the developing brain |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular Neuroscience |
issn |
1662-5102 |
publishDate |
2020-02-01 |
description |
The cerebral cortical tissue of murine embryo and pluripotent stem cell-derived neurons can survive in the adult brain and extend axons to the spinal cord. These features suggest that cell transplantation can be a strategy to reconstruct the corticospinal tract (CST). It is unknown, however, which cell population makes for safe and effective donor cells. To address this issue, we grafted the cerebral cortex of E14.5 mouse to the brain of adult mice and found that the cells in the graft extending axons along the CST expressed CTIP2. By using CTIP2:GFP knock-in mouse embryonic stem cells (mESCs), we identified L1CAM as a cell surface marker to enrich CTIP2+ cells. We sorted L1CAM+ cells from E14.5 mouse brain and confirmed that they extended a larger number of axons along the CST compared to L1CAM− cells. Our results suggest that sorting L1CAM+ cells from the embryonic cerebral cortex enriches subcortical projection neurons to reconstruct the CST. |
topic |
L1 cell adhesion molecule corticospinal motor neurons transplantation corticospinal tract cell sorting |
url |
https://www.frontiersin.org/article/10.3389/fncel.2020.00031/full |
work_keys_str_mv |
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