Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification
The use of TIRF microscopy for DNA-PAINT experiments is limited by inhomogeneous illumination. Here the authors show that quantitative analysis of single-molecule TIRF experiments can be improved by using a segment-wise analysis approach and overcome by using a beam-shaping device to give a flat-top...
Main Authors: | Florian Stehr, Johannes Stein, Florian Schueder, Petra Schwille, Ralf Jungmann |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2019-03-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-019-09064-6 |
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