Comparison of a New <sup>68</sup>Ga-Radiolabelled PET Imaging Agent sCD146 and RGD Peptide for In Vivo Evaluation of Angiogenesis in Mouse Model of Myocardial Infarction
Ischemic vascular diseases are associated with elevated tissue expression of angiomotin (AMOT), a promising molecular target for PET imaging. On that basis, we developed an AMOT-targeting radiotracer, <sup>68</sup>Ga-sCD146 and performed the first in vivo evaluation on a myocardial infar...
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doaj-eb0368dad97045d59d99c924c55f52f52021-09-25T23:52:28ZengMDPI AGCells2073-44092021-09-01102305230510.3390/cells10092305Comparison of a New <sup>68</sup>Ga-Radiolabelled PET Imaging Agent sCD146 and RGD Peptide for In Vivo Evaluation of Angiogenesis in Mouse Model of Myocardial InfarctionAnaïs Moyon0Philippe Garrigue1Samantha Fernandez2Fabien Hubert3Laure Balasse4Pauline Brige5Guillaume Hache6Vincent Nail7Marcel Blot-Chabaud8Françoise Dignat-George9Francesca Rochais10Benjamin Guillet11Pharmacological Faculty, Aix Marseille University, INSERM 1263, INRAE 1260, C2VN, 13385 Marseille, FrancePharmacological Faculty, Aix Marseille University, INSERM 1263, INRAE 1260, C2VN, 13385 Marseille, FranceMedical Faculty, Aix-Marseille University, CNRS 2012, CERIMED, 13385 Marseille, FranceMedical Faculty, Aix Marseille University, INSERM, MMG, U 1251, 13385 Marseille, FranceMedical Faculty, Aix-Marseille University, CNRS 2012, CERIMED, 13385 Marseille, FranceMedical Faculty, Aix-Marseille University, CNRS 2012, CERIMED, 13385 Marseille, FrancePharmacological Faculty, Aix Marseille University, INSERM 1263, INRAE 1260, C2VN, 13385 Marseille, FrancePharmacological Faculty, Aix Marseille University, INSERM 1263, INRAE 1260, C2VN, 13385 Marseille, FrancePharmacological Faculty, Aix Marseille University, INSERM 1263, INRAE 1260, C2VN, 13385 Marseille, FrancePharmacological Faculty, Aix Marseille University, INSERM 1263, INRAE 1260, C2VN, 13385 Marseille, FranceMedical Faculty, Aix Marseille University, INSERM, MMG, U 1251, 13385 Marseille, FrancePharmacological Faculty, Aix Marseille University, INSERM 1263, INRAE 1260, C2VN, 13385 Marseille, FranceIschemic vascular diseases are associated with elevated tissue expression of angiomotin (AMOT), a promising molecular target for PET imaging. On that basis, we developed an AMOT-targeting radiotracer, <sup>68</sup>Ga-sCD146 and performed the first in vivo evaluation on a myocardial infarction mice model and then, compared AMOT expression and α<sub>v</sub>β<sub>3</sub>-integrin expression with <sup>68</sup>Ga-sCD146 and <sup>68</sup>Ga-RGD<sub>2</sub> imaging. After myocardial infarction (MI) induced by permanent ligation of the left anterior descending coronary artery, myocardial perfusion was evaluated by Doppler ultrasound and by <sup>18</sup>F-FDG PET imaging. <sup>68</sup>Ga-sCD146 and <sup>68</sup>Ga-RGD<sub>2</sub> PET imaging were performed. In myocardial infarction model, heart-to-muscle ratio of <sup>68</sup>Ga-sCD146 imaging showed a significantly higher radiotracer uptake in the infarcted area of MI animals than in sham (* <i>p</i> = 0.04). Interestingly, we also observed significant correlations between <sup>68</sup>Ga-sCD146 imaging and delayed residual perfusion assessed by <sup>18</sup>F-FDG (* <i>p</i> = 0.04), with lowest tissue fibrosis assessed by histological staining (* <i>p</i> = 0.04) and with functional recovery assessed by ultrasound imaging (** <i>p</i> = 0.01). <sup>68</sup>Ga-sCD146 demonstrated an increase in AMOT expression after MI. Altogether, significant correlations of early post-ischemic <sup>68</sup>Ga-sCD146 uptake with late heart perfusion, lower tissue fibrosis and better functional recovery, make <sup>68</sup>Ga-sCD146 a promising radiotracer for tissue angiogenesis assessment after MI.https://www.mdpi.com/2073-4409/10/9/2305angiomotinmyocardial infarctionsCD146galliumangiogenesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anaïs Moyon Philippe Garrigue Samantha Fernandez Fabien Hubert Laure Balasse Pauline Brige Guillaume Hache Vincent Nail Marcel Blot-Chabaud Françoise Dignat-George Francesca Rochais Benjamin Guillet |
spellingShingle |
Anaïs Moyon Philippe Garrigue Samantha Fernandez Fabien Hubert Laure Balasse Pauline Brige Guillaume Hache Vincent Nail Marcel Blot-Chabaud Françoise Dignat-George Francesca Rochais Benjamin Guillet Comparison of a New <sup>68</sup>Ga-Radiolabelled PET Imaging Agent sCD146 and RGD Peptide for In Vivo Evaluation of Angiogenesis in Mouse Model of Myocardial Infarction Cells angiomotin myocardial infarction sCD146 gallium angiogenesis |
author_facet |
Anaïs Moyon Philippe Garrigue Samantha Fernandez Fabien Hubert Laure Balasse Pauline Brige Guillaume Hache Vincent Nail Marcel Blot-Chabaud Françoise Dignat-George Francesca Rochais Benjamin Guillet |
author_sort |
Anaïs Moyon |
title |
Comparison of a New <sup>68</sup>Ga-Radiolabelled PET Imaging Agent sCD146 and RGD Peptide for In Vivo Evaluation of Angiogenesis in Mouse Model of Myocardial Infarction |
title_short |
Comparison of a New <sup>68</sup>Ga-Radiolabelled PET Imaging Agent sCD146 and RGD Peptide for In Vivo Evaluation of Angiogenesis in Mouse Model of Myocardial Infarction |
title_full |
Comparison of a New <sup>68</sup>Ga-Radiolabelled PET Imaging Agent sCD146 and RGD Peptide for In Vivo Evaluation of Angiogenesis in Mouse Model of Myocardial Infarction |
title_fullStr |
Comparison of a New <sup>68</sup>Ga-Radiolabelled PET Imaging Agent sCD146 and RGD Peptide for In Vivo Evaluation of Angiogenesis in Mouse Model of Myocardial Infarction |
title_full_unstemmed |
Comparison of a New <sup>68</sup>Ga-Radiolabelled PET Imaging Agent sCD146 and RGD Peptide for In Vivo Evaluation of Angiogenesis in Mouse Model of Myocardial Infarction |
title_sort |
comparison of a new <sup>68</sup>ga-radiolabelled pet imaging agent scd146 and rgd peptide for in vivo evaluation of angiogenesis in mouse model of myocardial infarction |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2021-09-01 |
description |
Ischemic vascular diseases are associated with elevated tissue expression of angiomotin (AMOT), a promising molecular target for PET imaging. On that basis, we developed an AMOT-targeting radiotracer, <sup>68</sup>Ga-sCD146 and performed the first in vivo evaluation on a myocardial infarction mice model and then, compared AMOT expression and α<sub>v</sub>β<sub>3</sub>-integrin expression with <sup>68</sup>Ga-sCD146 and <sup>68</sup>Ga-RGD<sub>2</sub> imaging. After myocardial infarction (MI) induced by permanent ligation of the left anterior descending coronary artery, myocardial perfusion was evaluated by Doppler ultrasound and by <sup>18</sup>F-FDG PET imaging. <sup>68</sup>Ga-sCD146 and <sup>68</sup>Ga-RGD<sub>2</sub> PET imaging were performed. In myocardial infarction model, heart-to-muscle ratio of <sup>68</sup>Ga-sCD146 imaging showed a significantly higher radiotracer uptake in the infarcted area of MI animals than in sham (* <i>p</i> = 0.04). Interestingly, we also observed significant correlations between <sup>68</sup>Ga-sCD146 imaging and delayed residual perfusion assessed by <sup>18</sup>F-FDG (* <i>p</i> = 0.04), with lowest tissue fibrosis assessed by histological staining (* <i>p</i> = 0.04) and with functional recovery assessed by ultrasound imaging (** <i>p</i> = 0.01). <sup>68</sup>Ga-sCD146 demonstrated an increase in AMOT expression after MI. Altogether, significant correlations of early post-ischemic <sup>68</sup>Ga-sCD146 uptake with late heart perfusion, lower tissue fibrosis and better functional recovery, make <sup>68</sup>Ga-sCD146 a promising radiotracer for tissue angiogenesis assessment after MI. |
topic |
angiomotin myocardial infarction sCD146 gallium angiogenesis |
url |
https://www.mdpi.com/2073-4409/10/9/2305 |
work_keys_str_mv |
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