3D Cartilage Regeneration With Certain Shape and Mechanical Strength Based on Engineered Cartilage Gel and Decalcified Bone Matrix
Scaffold-free cartilage-sheet technology can stably regenerate high-quality cartilage tissue in vivo. However, uncontrolled shape maintenance and mechanical strength greatly hinder its clinical translation. Decalcified bone matrix (DBM) has high porosity, a suitable pore structure, and good biocompa...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.638115/full |
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doaj-eb09894ce8e340408af980fbec5294b4 |
|---|---|
| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Zheng Ci Zheng Ci Zheng Ci Ying Zhang Yahui Wang Yahui Wang Yahui Wang Gaoyang Wu Gaoyang Wu Mengjie Hou Mengjie Hou Peiling Zhang Peiling Zhang Litao Jia Litao Jia Baoshuai Bai Baoshuai Bai Baoshuai Bai Yilin Cao Yilin Cao Yilin Cao Yu Liu Yu Liu Yu Liu Guangdong Zhou Guangdong Zhou Guangdong Zhou |
| spellingShingle |
Zheng Ci Zheng Ci Zheng Ci Ying Zhang Yahui Wang Yahui Wang Yahui Wang Gaoyang Wu Gaoyang Wu Mengjie Hou Mengjie Hou Peiling Zhang Peiling Zhang Litao Jia Litao Jia Baoshuai Bai Baoshuai Bai Baoshuai Bai Yilin Cao Yilin Cao Yilin Cao Yu Liu Yu Liu Yu Liu Guangdong Zhou Guangdong Zhou Guangdong Zhou 3D Cartilage Regeneration With Certain Shape and Mechanical Strength Based on Engineered Cartilage Gel and Decalcified Bone Matrix Frontiers in Cell and Developmental Biology 3D cartilage regeneration engineered cartilage gel decalcified bone matrix tissue engineering nutrient efficiency |
| author_facet |
Zheng Ci Zheng Ci Zheng Ci Ying Zhang Yahui Wang Yahui Wang Yahui Wang Gaoyang Wu Gaoyang Wu Mengjie Hou Mengjie Hou Peiling Zhang Peiling Zhang Litao Jia Litao Jia Baoshuai Bai Baoshuai Bai Baoshuai Bai Yilin Cao Yilin Cao Yilin Cao Yu Liu Yu Liu Yu Liu Guangdong Zhou Guangdong Zhou Guangdong Zhou |
| author_sort |
Zheng Ci |
| title |
3D Cartilage Regeneration With Certain Shape and Mechanical Strength Based on Engineered Cartilage Gel and Decalcified Bone Matrix |
| title_short |
3D Cartilage Regeneration With Certain Shape and Mechanical Strength Based on Engineered Cartilage Gel and Decalcified Bone Matrix |
| title_full |
3D Cartilage Regeneration With Certain Shape and Mechanical Strength Based on Engineered Cartilage Gel and Decalcified Bone Matrix |
| title_fullStr |
3D Cartilage Regeneration With Certain Shape and Mechanical Strength Based on Engineered Cartilage Gel and Decalcified Bone Matrix |
| title_full_unstemmed |
3D Cartilage Regeneration With Certain Shape and Mechanical Strength Based on Engineered Cartilage Gel and Decalcified Bone Matrix |
| title_sort |
3d cartilage regeneration with certain shape and mechanical strength based on engineered cartilage gel and decalcified bone matrix |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Cell and Developmental Biology |
| issn |
2296-634X |
| publishDate |
2021-02-01 |
| description |
Scaffold-free cartilage-sheet technology can stably regenerate high-quality cartilage tissue in vivo. However, uncontrolled shape maintenance and mechanical strength greatly hinder its clinical translation. Decalcified bone matrix (DBM) has high porosity, a suitable pore structure, and good biocompatibility, as well as controlled shape and mechanical strength. In this study, cartilage sheet was prepared into engineered cartilage gel (ECG) and combined with DBM to explore the feasibility of regenerating 3D cartilage with controlled shape and mechanical strength. The results indicated that ECG cultured in vitro for 3 days (3 d) and 15 days (15 d) showed good biocompatibility with DBM, and the ECG–DBM constructs successfully regenerated viable 3D cartilage with typical mature cartilage features in both nude mice and autologous goats. Additionally, the regenerated cartilage had comparable mechanical properties to native cartilage and maintained its original shape. To further determine the optimal seeding parameters for ECG, the 3 d ECG regenerated using human chondrocytes was diluted in different concentrations (1:3, 1:2, and 1:1) for seeding and in vivo implantation. The results showed that the regenerated cartilage in the 1:2 group exhibited better shape maintenance and homogeneity than the other groups. The current study established a novel mode of 3D cartilage regeneration based on the design concept of steel (DBM)-reinforced concrete (ECG) and successfully regenerated homogenous and mature 3D cartilage with controlled shape and mechanical strength, which hopefully provides an ideal cartilage graft for the repair of various cartilage defects. |
| topic |
3D cartilage regeneration engineered cartilage gel decalcified bone matrix tissue engineering nutrient efficiency |
| url |
https://www.frontiersin.org/articles/10.3389/fcell.2021.638115/full |
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doaj-eb09894ce8e340408af980fbec5294b42021-02-26T05:06:55ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-02-01910.3389/fcell.2021.6381156381153D Cartilage Regeneration With Certain Shape and Mechanical Strength Based on Engineered Cartilage Gel and Decalcified Bone MatrixZheng Ci0Zheng Ci1Zheng Ci2Ying Zhang3Yahui Wang4Yahui Wang5Yahui Wang6Gaoyang Wu7Gaoyang Wu8Mengjie Hou9Mengjie Hou10Peiling Zhang11Peiling Zhang12Litao Jia13Litao Jia14Baoshuai Bai15Baoshuai Bai16Baoshuai Bai17Yilin Cao18Yilin Cao19Yilin Cao20Yu Liu21Yu Liu22Yu Liu23Guangdong Zhou24Guangdong Zhou25Guangdong Zhou26Research Institute of Plastic Surgery, Wei Fang Medical College, Wei Fang, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaResearch Institute of Plastic Surgery, Wei Fang Medical College, Wei Fang, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaPlastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaResearch Institute of Plastic Surgery, Wei Fang Medical College, Wei Fang, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaResearch Institute of Plastic Surgery, Wei Fang Medical College, Wei Fang, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaResearch Institute of Plastic Surgery, Wei Fang Medical College, Wei Fang, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaResearch Institute of Plastic Surgery, Wei Fang Medical College, Wei Fang, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaScaffold-free cartilage-sheet technology can stably regenerate high-quality cartilage tissue in vivo. However, uncontrolled shape maintenance and mechanical strength greatly hinder its clinical translation. Decalcified bone matrix (DBM) has high porosity, a suitable pore structure, and good biocompatibility, as well as controlled shape and mechanical strength. In this study, cartilage sheet was prepared into engineered cartilage gel (ECG) and combined with DBM to explore the feasibility of regenerating 3D cartilage with controlled shape and mechanical strength. The results indicated that ECG cultured in vitro for 3 days (3 d) and 15 days (15 d) showed good biocompatibility with DBM, and the ECG–DBM constructs successfully regenerated viable 3D cartilage with typical mature cartilage features in both nude mice and autologous goats. Additionally, the regenerated cartilage had comparable mechanical properties to native cartilage and maintained its original shape. To further determine the optimal seeding parameters for ECG, the 3 d ECG regenerated using human chondrocytes was diluted in different concentrations (1:3, 1:2, and 1:1) for seeding and in vivo implantation. The results showed that the regenerated cartilage in the 1:2 group exhibited better shape maintenance and homogeneity than the other groups. The current study established a novel mode of 3D cartilage regeneration based on the design concept of steel (DBM)-reinforced concrete (ECG) and successfully regenerated homogenous and mature 3D cartilage with controlled shape and mechanical strength, which hopefully provides an ideal cartilage graft for the repair of various cartilage defects.https://www.frontiersin.org/articles/10.3389/fcell.2021.638115/full3D cartilage regenerationengineered cartilage geldecalcified bone matrixtissue engineeringnutrient efficiency |