Osteoprotective Effect of Radix Scutellariae in Female Hindlimb-Suspended Sprague-Dawley Rats and the Osteogenic Differentiation Effect of Its Major Constituent
A number of medicinal herbs have demonstrated therapeutic effects for the prevention and treatment of disuse-induced osteoporosis. As a common ingredient in proprietary traditional Chinese medicines, the anti-osteoporosis effects of Radix Scutellariae extract (RSE, 50 mg/kg/day) were evaluated in a...
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doaj-eb16dcbbb07645a6acdd284b02de06972020-11-25T02:31:02ZengMDPI AGMolecules1420-30492017-07-01227104410.3390/molecules22071044molecules22071044Osteoprotective Effect of Radix Scutellariae in Female Hindlimb-Suspended Sprague-Dawley Rats and the Osteogenic Differentiation Effect of Its Major ConstituentGuangwei Zhang0Chenrui Li1Yinbo Niu2Qi Yu3Yulong Chen4Enqi Liu5Research Institute of Atherosclerotic Disease, College of Clinical Medicine, Xi’an Medical University, No.1Xinwang Road, Xi’an 710021, ChinaKey Laboratory for Space Biosciences & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyixi Road, Xi’an 710072, ChinaKey Laboratory for Space Biosciences & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyixi Road, Xi’an 710072, ChinaResearch Institute of Atherosclerotic Disease, College of Clinical Medicine, Xi’an Medical University, No.1Xinwang Road, Xi’an 710021, ChinaResearch Institute of Atherosclerotic Disease, College of Clinical Medicine, Xi’an Medical University, No.1Xinwang Road, Xi’an 710021, ChinaResearch Institute of Atherosclerotic Disease, College of Clinical Medicine, Xi’an Medical University, No.1Xinwang Road, Xi’an 710021, ChinaA number of medicinal herbs have demonstrated therapeutic effects for the prevention and treatment of disuse-induced osteoporosis. As a common ingredient in proprietary traditional Chinese medicines, the anti-osteoporosis effects of Radix Scutellariae extract (RSE, 50 mg/kg/day) were evaluated in a hindlimb suspended rat model. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry, and the micro-architecture observed by MicroCT assay with bone biomechanical properties evaluated by a three-point bending test. To elucidate potential mechanisms, the osteogenic differentiation effect of baicalin as the most abundant ingredient in RSE was investigated in rat bone marrow derived mesenchymal stem cells (rBMSC). After drug administration for 42 days, tibia-BMD was significantly increased to 0.176 ± 0.007 and 0.183 ± 0.011 g/cm2 and f-BMD was enhanced to 0.200 ± 0.017 and 0.207 ± 0.021 g/cm2 for RSE and ALE treatment, respectively, whereas tibia-BMD and femur-BMD of the HLS group were 0.157 ± 0.009 and 0.176 ± 0.008 g/cm2. Deterioration of bone trabecula microstructure was improved by RSE and ALE with increased morphological parameters such as bone volume fraction, trabecular thickness, and trabecular number, as well as connectivity density compared to the HLS group (p < 0.01). A three-point bending test suggested that bone mechanical strength was also enhanced by RSE and ALE treatments with increased maximum stress, young’s modulus, maximum load, and stiffness compared to those of the HLS group (p < 0.05). Besides, serum TRACP levels were significantly suppressed by RSE and ALE treatments. Furthermore, in vitro studies demonstrated that baicalin significantly increased ALP activities and the formation of mineralized nodules in rBMSC. Conclusively, supplementation of RSE could significantly prevent weightlessness induced osteoporosis, which might attribute to the osteogenic differentiation enhancement effect of baicalin.https://www.mdpi.com/1420-3049/22/7/1044Radix Scutellariaebaicalindisuse-induced osteoporosisosteogenic differentiationbone marrow derived mesenchymal stem cells |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guangwei Zhang Chenrui Li Yinbo Niu Qi Yu Yulong Chen Enqi Liu |
spellingShingle |
Guangwei Zhang Chenrui Li Yinbo Niu Qi Yu Yulong Chen Enqi Liu Osteoprotective Effect of Radix Scutellariae in Female Hindlimb-Suspended Sprague-Dawley Rats and the Osteogenic Differentiation Effect of Its Major Constituent Molecules Radix Scutellariae baicalin disuse-induced osteoporosis osteogenic differentiation bone marrow derived mesenchymal stem cells |
author_facet |
Guangwei Zhang Chenrui Li Yinbo Niu Qi Yu Yulong Chen Enqi Liu |
author_sort |
Guangwei Zhang |
title |
Osteoprotective Effect of Radix Scutellariae in Female Hindlimb-Suspended Sprague-Dawley Rats and the Osteogenic Differentiation Effect of Its Major Constituent |
title_short |
Osteoprotective Effect of Radix Scutellariae in Female Hindlimb-Suspended Sprague-Dawley Rats and the Osteogenic Differentiation Effect of Its Major Constituent |
title_full |
Osteoprotective Effect of Radix Scutellariae in Female Hindlimb-Suspended Sprague-Dawley Rats and the Osteogenic Differentiation Effect of Its Major Constituent |
title_fullStr |
Osteoprotective Effect of Radix Scutellariae in Female Hindlimb-Suspended Sprague-Dawley Rats and the Osteogenic Differentiation Effect of Its Major Constituent |
title_full_unstemmed |
Osteoprotective Effect of Radix Scutellariae in Female Hindlimb-Suspended Sprague-Dawley Rats and the Osteogenic Differentiation Effect of Its Major Constituent |
title_sort |
osteoprotective effect of radix scutellariae in female hindlimb-suspended sprague-dawley rats and the osteogenic differentiation effect of its major constituent |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2017-07-01 |
description |
A number of medicinal herbs have demonstrated therapeutic effects for the prevention and treatment of disuse-induced osteoporosis. As a common ingredient in proprietary traditional Chinese medicines, the anti-osteoporosis effects of Radix Scutellariae extract (RSE, 50 mg/kg/day) were evaluated in a hindlimb suspended rat model. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry, and the micro-architecture observed by MicroCT assay with bone biomechanical properties evaluated by a three-point bending test. To elucidate potential mechanisms, the osteogenic differentiation effect of baicalin as the most abundant ingredient in RSE was investigated in rat bone marrow derived mesenchymal stem cells (rBMSC). After drug administration for 42 days, tibia-BMD was significantly increased to 0.176 ± 0.007 and 0.183 ± 0.011 g/cm2 and f-BMD was enhanced to 0.200 ± 0.017 and 0.207 ± 0.021 g/cm2 for RSE and ALE treatment, respectively, whereas tibia-BMD and femur-BMD of the HLS group were 0.157 ± 0.009 and 0.176 ± 0.008 g/cm2. Deterioration of bone trabecula microstructure was improved by RSE and ALE with increased morphological parameters such as bone volume fraction, trabecular thickness, and trabecular number, as well as connectivity density compared to the HLS group (p < 0.01). A three-point bending test suggested that bone mechanical strength was also enhanced by RSE and ALE treatments with increased maximum stress, young’s modulus, maximum load, and stiffness compared to those of the HLS group (p < 0.05). Besides, serum TRACP levels were significantly suppressed by RSE and ALE treatments. Furthermore, in vitro studies demonstrated that baicalin significantly increased ALP activities and the formation of mineralized nodules in rBMSC. Conclusively, supplementation of RSE could significantly prevent weightlessness induced osteoporosis, which might attribute to the osteogenic differentiation enhancement effect of baicalin. |
topic |
Radix Scutellariae baicalin disuse-induced osteoporosis osteogenic differentiation bone marrow derived mesenchymal stem cells |
url |
https://www.mdpi.com/1420-3049/22/7/1044 |
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