Searching for Potential Markers of Glomerulopathy in Urine by HS-SPME-GC×GC TOFMS
Volatile organic compounds (VOCs) exiting in urine are potential biomarkers of chronic kidney diseases. Headspace solid phase microextraction (HS-SPME) was applied for extraction VOCs over the urine samples. Volatile metabolites were separated and identified by means of two-dimensional gas chromatog...
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doaj-eb4b4cbfae494fedabfb4018f60f56d22021-03-25T00:02:15ZengMDPI AGMolecules1420-30492021-03-01261817181710.3390/molecules26071817Searching for Potential Markers of Glomerulopathy in Urine by HS-SPME-GC×GC TOFMSTomasz Ligor0Joanna Zawadzka1Grzegorz Strączyński2Rosa M. González Paredes3Anna Wenda-Piesik4Ileana Andreea Ratiu5Marek Muszytowski6Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 87-100 Toruń, PolandDepartment of Nephrology, Diabetology and Internal Medicine, Nicolaus Copernicus University, Rydygier Hospital, 87-100 Toruń, PolandUSL Ltd., 43-110 Tychy, PolandDepartment of Analytical Chemistry, Nutrition and Food Sciences, University of Salamanca, 37008 Salamanca, SpainDepartment of Plant Growth Principles and Experimental Methods, UTP University of Science and Technology, 85-796 Bydgoszcz, PolandInterdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 87-100 Toruń, PolandDepartment of Nephrology, Diabetology and Internal Medicine, Nicolaus Copernicus University, Rydygier Hospital, 87-100 Toruń, PolandVolatile organic compounds (VOCs) exiting in urine are potential biomarkers of chronic kidney diseases. Headspace solid phase microextraction (HS-SPME) was applied for extraction VOCs over the urine samples. Volatile metabolites were separated and identified by means of two-dimensional gas chromatography and time of flight mass spectrometry (GC × GC TOF MS). Patients with glomerular diseases (<i>n</i> = 27) and healthy controls (<i>n</i> = 20) were recruited in the study. Different VOCs profiles were obtained from patients and control. Developed methodology offers the opportunity to examine the metabolic profile associated with glomerulopathy. Four compounds found in elevated amounts in the patients group, i.e., methyl hexadecanoate; 9-hexadecen-1-ol; 6,10-dimethyl-5,9-undecadien-2-one and 2-pentanone were proposed as markers of glomerular diseases.https://www.mdpi.com/1420-3049/26/7/1817volatile organic compoundsurine analysiscomprehensive two-dimensional gas chromatographykidney diseases |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tomasz Ligor Joanna Zawadzka Grzegorz Strączyński Rosa M. González Paredes Anna Wenda-Piesik Ileana Andreea Ratiu Marek Muszytowski |
spellingShingle |
Tomasz Ligor Joanna Zawadzka Grzegorz Strączyński Rosa M. González Paredes Anna Wenda-Piesik Ileana Andreea Ratiu Marek Muszytowski Searching for Potential Markers of Glomerulopathy in Urine by HS-SPME-GC×GC TOFMS Molecules volatile organic compounds urine analysis comprehensive two-dimensional gas chromatography kidney diseases |
author_facet |
Tomasz Ligor Joanna Zawadzka Grzegorz Strączyński Rosa M. González Paredes Anna Wenda-Piesik Ileana Andreea Ratiu Marek Muszytowski |
author_sort |
Tomasz Ligor |
title |
Searching for Potential Markers of Glomerulopathy in Urine by HS-SPME-GC×GC TOFMS |
title_short |
Searching for Potential Markers of Glomerulopathy in Urine by HS-SPME-GC×GC TOFMS |
title_full |
Searching for Potential Markers of Glomerulopathy in Urine by HS-SPME-GC×GC TOFMS |
title_fullStr |
Searching for Potential Markers of Glomerulopathy in Urine by HS-SPME-GC×GC TOFMS |
title_full_unstemmed |
Searching for Potential Markers of Glomerulopathy in Urine by HS-SPME-GC×GC TOFMS |
title_sort |
searching for potential markers of glomerulopathy in urine by hs-spme-gc×gc tofms |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-03-01 |
description |
Volatile organic compounds (VOCs) exiting in urine are potential biomarkers of chronic kidney diseases. Headspace solid phase microextraction (HS-SPME) was applied for extraction VOCs over the urine samples. Volatile metabolites were separated and identified by means of two-dimensional gas chromatography and time of flight mass spectrometry (GC × GC TOF MS). Patients with glomerular diseases (<i>n</i> = 27) and healthy controls (<i>n</i> = 20) were recruited in the study. Different VOCs profiles were obtained from patients and control. Developed methodology offers the opportunity to examine the metabolic profile associated with glomerulopathy. Four compounds found in elevated amounts in the patients group, i.e., methyl hexadecanoate; 9-hexadecen-1-ol; 6,10-dimethyl-5,9-undecadien-2-one and 2-pentanone were proposed as markers of glomerular diseases. |
topic |
volatile organic compounds urine analysis comprehensive two-dimensional gas chromatography kidney diseases |
url |
https://www.mdpi.com/1420-3049/26/7/1817 |
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