Prognostic Significance of Cyclin E1 Expression in Patients With Chordoma: A Clinicopathological and Immunohistochemical Study
PurposeChordomas are rare, slow-growing sarcomas without any accepted prognostic biomarkers. Owing to their proximity to critical neurovascular structures, discovering predictive biomarkers in chordoma has been a significant research effort because it may potentially reduce risky therapies in patien...
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doaj-eb53ec3fc05a48059616c95aa01a52062020-11-25T03:08:32ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-11-011010.3389/fonc.2020.596330596330Prognostic Significance of Cyclin E1 Expression in Patients With Chordoma: A Clinicopathological and Immunohistochemical StudyRan Wei0Ran Wei1Dylan C. Dean2Pichaya Thanindratarn3Pichaya Thanindratarn4Francis J. Hornicek5Wei Guo6Zhenfeng Duan7Musculoskeletal Tumor Center, Beijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaSarcoma Biology Laboratory, Department of Orthopedic Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, United StatesSarcoma Biology Laboratory, Department of Orthopedic Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, United StatesSarcoma Biology Laboratory, Department of Orthopedic Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, United StatesDepartment of Orthopedic Surgery, Chulabhorn Hospital, HRH Princess Chulabhorn College of Medical Science, Bangkok, ThailandSarcoma Biology Laboratory, Department of Orthopedic Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, United StatesMusculoskeletal Tumor Center, Beijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing, ChinaSarcoma Biology Laboratory, Department of Orthopedic Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, United StatesPurposeChordomas are rare, slow-growing sarcomas without any accepted prognostic biomarkers. Owing to their proximity to critical neurovascular structures, discovering predictive biomarkers in chordoma has been a significant research effort because it may potentially reduce risky therapies in patients with less aggressive tumors. In response, because cyclin E1 overexpression correlates with patient prognosis in several malignancies, we investigated its expression in chordoma and whether it informs patient prognosis.MethodsSeventy-five chordoma patient specimens were enrolled in a tissue microarray (TMA) to evaluate cyclin E1 expression via immunohistochemical staining. Western blot was used to assess cyclin E1 expression in chordoma cell lines and fresh tissues. We then correlated cyclin E1 staining intensity in the TMA to clinicopathological features and chordoma patient outcomes.ResultsSixty-three percent of the chordoma patient specimens in the TMA, fifty-six percent of the fresh chordoma tissues, and all chordoma cell lines showed high cyclin E1 expression. In TMA analysis, cyclin E1 expression positively correlated to chordoma patient disease status. By survival analysis, high cyclin E1 expression was an independent prognostic risk factor for chordoma patients along with advanced disease status and positive surgical margin.ConclusionCyclin E1 is a promising biomarker predicting chordoma patient prognosis.https://www.frontiersin.org/articles/10.3389/fonc.2020.596330/fullchordomacyclin E1prognosisbiomarkertissue microarrayimmunohistochemical staining |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ran Wei Ran Wei Dylan C. Dean Pichaya Thanindratarn Pichaya Thanindratarn Francis J. Hornicek Wei Guo Zhenfeng Duan |
spellingShingle |
Ran Wei Ran Wei Dylan C. Dean Pichaya Thanindratarn Pichaya Thanindratarn Francis J. Hornicek Wei Guo Zhenfeng Duan Prognostic Significance of Cyclin E1 Expression in Patients With Chordoma: A Clinicopathological and Immunohistochemical Study Frontiers in Oncology chordoma cyclin E1 prognosis biomarker tissue microarray immunohistochemical staining |
author_facet |
Ran Wei Ran Wei Dylan C. Dean Pichaya Thanindratarn Pichaya Thanindratarn Francis J. Hornicek Wei Guo Zhenfeng Duan |
author_sort |
Ran Wei |
title |
Prognostic Significance of Cyclin E1 Expression in Patients With Chordoma: A Clinicopathological and Immunohistochemical Study |
title_short |
Prognostic Significance of Cyclin E1 Expression in Patients With Chordoma: A Clinicopathological and Immunohistochemical Study |
title_full |
Prognostic Significance of Cyclin E1 Expression in Patients With Chordoma: A Clinicopathological and Immunohistochemical Study |
title_fullStr |
Prognostic Significance of Cyclin E1 Expression in Patients With Chordoma: A Clinicopathological and Immunohistochemical Study |
title_full_unstemmed |
Prognostic Significance of Cyclin E1 Expression in Patients With Chordoma: A Clinicopathological and Immunohistochemical Study |
title_sort |
prognostic significance of cyclin e1 expression in patients with chordoma: a clinicopathological and immunohistochemical study |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2020-11-01 |
description |
PurposeChordomas are rare, slow-growing sarcomas without any accepted prognostic biomarkers. Owing to their proximity to critical neurovascular structures, discovering predictive biomarkers in chordoma has been a significant research effort because it may potentially reduce risky therapies in patients with less aggressive tumors. In response, because cyclin E1 overexpression correlates with patient prognosis in several malignancies, we investigated its expression in chordoma and whether it informs patient prognosis.MethodsSeventy-five chordoma patient specimens were enrolled in a tissue microarray (TMA) to evaluate cyclin E1 expression via immunohistochemical staining. Western blot was used to assess cyclin E1 expression in chordoma cell lines and fresh tissues. We then correlated cyclin E1 staining intensity in the TMA to clinicopathological features and chordoma patient outcomes.ResultsSixty-three percent of the chordoma patient specimens in the TMA, fifty-six percent of the fresh chordoma tissues, and all chordoma cell lines showed high cyclin E1 expression. In TMA analysis, cyclin E1 expression positively correlated to chordoma patient disease status. By survival analysis, high cyclin E1 expression was an independent prognostic risk factor for chordoma patients along with advanced disease status and positive surgical margin.ConclusionCyclin E1 is a promising biomarker predicting chordoma patient prognosis. |
topic |
chordoma cyclin E1 prognosis biomarker tissue microarray immunohistochemical staining |
url |
https://www.frontiersin.org/articles/10.3389/fonc.2020.596330/full |
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