Electrochemical Characterisation and Application of Multi Microelectrode Array Devices to Biological Electrochemistry

• Main Authors: William S. McIntire, Peter A. Leigh, Peter J. Dobson, H. Allen O. Hill, Michael Kudera
• Format: Article
• Language: English
• Published: MDPI AG 2001-06-01
• Series: Sensors
• Subjects: Microelectrode array; bioelectrochemical sensor; direct protein electrochemistry; cytochrome c; amicyanin
• Online Access: http://www.mdpi.com/1424-8220/1/1/18/
• ISSN: 1424-8220

A new design for microelectrode array (MEA) devices fabricated by semiconductor-processing techniques is presented. The microelectrode surfaces consist of gold and are surrounded by an insulating silicon nitride layer. Each chip of these so-called Multi MEAs contains regular arrays with circular-shaped electrodes of eight different sizes: 1, 3, 5, 10, 50, 100, 500 and 1000μm. The Multi MEAs were electrochemically characterised by use of ferrocenecarboxylic acid. Well-defined cyclic voltammograms of the two small redox proteins, horse heart cytochrome c and amicyanin from Thiobacillus versutus, were obtained at variously surface-modified Multi MEAs. Furthermore, a very simple method to manufacture Multi MEAs with carbon surfaces is introduced.