Evaluation of Neuroprotective Effect of Thymoquinone Nanoformulation in the Rodent Cerebral Ischemia-Reperfusion Model
The purpose of the present study was to evaluate the neuroprotective efficacy of optimized thymoquinone loaded PLGA-chitosan nanoparticles delivered via nose to brain route in the rodent cerebral ischemia-reperfusion model. The neuroprotective efficacy of the optimized thymoquinone loaded PLGA-chito...
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Online Access: | http://dx.doi.org/10.1155/2016/2571060 |
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doaj-eb639564f65f4bc69e763f0d60e1e82b2020-11-24T21:19:57ZengHindawi LimitedBioMed Research International2314-61332314-61412016-01-01201610.1155/2016/25710602571060Evaluation of Neuroprotective Effect of Thymoquinone Nanoformulation in the Rodent Cerebral Ischemia-Reperfusion ModelXiao-Yu Xiao0Ying-Xian Zhu1Ju-Yuan Bu2Guo-Wei Li3Jian-Hui Zhou4Shao-Peng Zhou5Department of Anaesthesiology, Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, Guangdong 519000, ChinaDepartment of Anaesthesiology, Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, Guangdong 519000, ChinaDepartment of Gastrointestinal Surgery, Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, Guangdong 519000, ChinaDepartment of Spine Surgery, Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, Guangdong 519000, ChinaDepartment of Laboratory, Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, Guangdong 519000, ChinaDepartment of Anaesthesiology, Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, Guangdong 519000, ChinaThe purpose of the present study was to evaluate the neuroprotective efficacy of optimized thymoquinone loaded PLGA-chitosan nanoparticles delivered via nose to brain route in the rodent cerebral ischemia-reperfusion model. The neuroprotective efficacy of the optimized thymoquinone loaded PLGA-chitosan nanoparticles was evaluated in middle cerebral artery occluded rats by various pharmacodynamic and biochemical studies. The pharmacokinetics of thymoquinone loaded PLGA-chitosan nanoparticles in the brain and blood plasma together with qualitative localization of florescent labelled PLGA-chitosan nanoparticles in brain tissues were also determined. Intranasal delivery of optimized thymoquinone loaded PLGA-chitosan nanoparticles (183.5±8.2 nm, 33.63±2.25 mV) to brain significantly reduced the ischemia infarct volume and enhanced the locomotor activity and grip strength in the middle cerebral artery occluded rats. Biochemical studies showed that intranasal delivery of thymoquinone loaded PLGA-chitosan nanoparticles significantly reduced the lipid peroxidation but elevated the glutathione, catalase, and superoxide dismutase in the brain of middle cerebral artery occluded rats. The pharmacokinetic and localization studies showed that thymoquinone loaded PLGA-chitosan nanoparticles facilitated the delivery of thymoquinone to brain by intranasal nose to brain transport pathways and enhanced their pharmacokinetic profile in brain tissues. Thus, intranasal delivery of thymoquinone loaded PLGA-chitosan nanoparticles to brain could be potentially used for the neuroprotection and treatment of cerebral ischemia.http://dx.doi.org/10.1155/2016/2571060 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiao-Yu Xiao Ying-Xian Zhu Ju-Yuan Bu Guo-Wei Li Jian-Hui Zhou Shao-Peng Zhou |
spellingShingle |
Xiao-Yu Xiao Ying-Xian Zhu Ju-Yuan Bu Guo-Wei Li Jian-Hui Zhou Shao-Peng Zhou Evaluation of Neuroprotective Effect of Thymoquinone Nanoformulation in the Rodent Cerebral Ischemia-Reperfusion Model BioMed Research International |
author_facet |
Xiao-Yu Xiao Ying-Xian Zhu Ju-Yuan Bu Guo-Wei Li Jian-Hui Zhou Shao-Peng Zhou |
author_sort |
Xiao-Yu Xiao |
title |
Evaluation of Neuroprotective Effect of Thymoquinone Nanoformulation in the Rodent Cerebral Ischemia-Reperfusion Model |
title_short |
Evaluation of Neuroprotective Effect of Thymoquinone Nanoformulation in the Rodent Cerebral Ischemia-Reperfusion Model |
title_full |
Evaluation of Neuroprotective Effect of Thymoquinone Nanoformulation in the Rodent Cerebral Ischemia-Reperfusion Model |
title_fullStr |
Evaluation of Neuroprotective Effect of Thymoquinone Nanoformulation in the Rodent Cerebral Ischemia-Reperfusion Model |
title_full_unstemmed |
Evaluation of Neuroprotective Effect of Thymoquinone Nanoformulation in the Rodent Cerebral Ischemia-Reperfusion Model |
title_sort |
evaluation of neuroprotective effect of thymoquinone nanoformulation in the rodent cerebral ischemia-reperfusion model |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2016-01-01 |
description |
The purpose of the present study was to evaluate the neuroprotective efficacy of optimized thymoquinone loaded PLGA-chitosan nanoparticles delivered via nose to brain route in the rodent cerebral ischemia-reperfusion model. The neuroprotective efficacy of the optimized thymoquinone loaded PLGA-chitosan nanoparticles was evaluated in middle cerebral artery occluded rats by various pharmacodynamic and biochemical studies. The pharmacokinetics of thymoquinone loaded PLGA-chitosan nanoparticles in the brain and blood plasma together with qualitative localization of florescent labelled PLGA-chitosan nanoparticles in brain tissues were also determined. Intranasal delivery of optimized thymoquinone loaded PLGA-chitosan nanoparticles (183.5±8.2 nm, 33.63±2.25 mV) to brain significantly reduced the ischemia infarct volume and enhanced the locomotor activity and grip strength in the middle cerebral artery occluded rats. Biochemical studies showed that intranasal delivery of thymoquinone loaded PLGA-chitosan nanoparticles significantly reduced the lipid peroxidation but elevated the glutathione, catalase, and superoxide dismutase in the brain of middle cerebral artery occluded rats. The pharmacokinetic and localization studies showed that thymoquinone loaded PLGA-chitosan nanoparticles facilitated the delivery of thymoquinone to brain by intranasal nose to brain transport pathways and enhanced their pharmacokinetic profile in brain tissues. Thus, intranasal delivery of thymoquinone loaded PLGA-chitosan nanoparticles to brain could be potentially used for the neuroprotection and treatment of cerebral ischemia. |
url |
http://dx.doi.org/10.1155/2016/2571060 |
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