CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment
Abstract Background Spindle microtubule organization, regulated by microtubule-associated proteins, is critical for cell division. Proper organization of kinetochore fiber (K-fiber), connecting spindle poles and kinetochores, is a prerequisite for precise chromosomal alignment and faithful genetic m...
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doaj-eb9c5a006c1642bf9f5c4917a02de8f62020-11-25T03:05:32ZengBMCBMC Biology1741-70072019-09-0117111410.1186/s12915-019-0694-9CCDC74A/B are K-fiber crosslinkers required for chromosomal alignmentHaining Zhou0Tao Zheng1Tianning Wang2Qi Li3Fulin Wang4Xin Liang5Jianguo Chen6Junlin Teng7Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking UniversityKey Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking UniversityKey Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking UniversityKey Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking UniversityKey Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking UniversityPeking-Tsinghua Center for Life Sciences and Max-Planck Partner Group, School of Life Sciences, Tsinghua UniversityKey Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking UniversityKey Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking UniversityAbstract Background Spindle microtubule organization, regulated by microtubule-associated proteins, is critical for cell division. Proper organization of kinetochore fiber (K-fiber), connecting spindle poles and kinetochores, is a prerequisite for precise chromosomal alignment and faithful genetic material transmission. However, the mechanisms of K-fiber organization and dynamic maintenance are still not fully understood. Results We reveal that two previously uncharacterized coiled-coil domain proteins CCDC74A and CCDC74B (CCDC74A/B) are spindle-localized proteins in mammalian cells. They bind directly to microtubules through two separate domains and bundle microtubules both in vivo and in vitro. These functions are required for K-fiber organization, bipolar spindle formation, and chromosomal alignment. Moreover, CCDC74A/B form homodimers in vivo, and their self-association activity is necessary for microtubule bundling and K-fiber formation. Conclusions We characterize CCDC74A and CCDC74B as microtubule-associated proteins that localize to spindles and are important K-fiber crosslinkers required for bipolar spindle formation and chromosome alignment.http://link.springer.com/article/10.1186/s12915-019-0694-9CCDC74ACCDC74BKinetochore fiberMicrotubule dynamicSpindle assemblyChromosomal alignment |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haining Zhou Tao Zheng Tianning Wang Qi Li Fulin Wang Xin Liang Jianguo Chen Junlin Teng |
spellingShingle |
Haining Zhou Tao Zheng Tianning Wang Qi Li Fulin Wang Xin Liang Jianguo Chen Junlin Teng CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment BMC Biology CCDC74A CCDC74B Kinetochore fiber Microtubule dynamic Spindle assembly Chromosomal alignment |
author_facet |
Haining Zhou Tao Zheng Tianning Wang Qi Li Fulin Wang Xin Liang Jianguo Chen Junlin Teng |
author_sort |
Haining Zhou |
title |
CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment |
title_short |
CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment |
title_full |
CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment |
title_fullStr |
CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment |
title_full_unstemmed |
CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment |
title_sort |
ccdc74a/b are k-fiber crosslinkers required for chromosomal alignment |
publisher |
BMC |
series |
BMC Biology |
issn |
1741-7007 |
publishDate |
2019-09-01 |
description |
Abstract Background Spindle microtubule organization, regulated by microtubule-associated proteins, is critical for cell division. Proper organization of kinetochore fiber (K-fiber), connecting spindle poles and kinetochores, is a prerequisite for precise chromosomal alignment and faithful genetic material transmission. However, the mechanisms of K-fiber organization and dynamic maintenance are still not fully understood. Results We reveal that two previously uncharacterized coiled-coil domain proteins CCDC74A and CCDC74B (CCDC74A/B) are spindle-localized proteins in mammalian cells. They bind directly to microtubules through two separate domains and bundle microtubules both in vivo and in vitro. These functions are required for K-fiber organization, bipolar spindle formation, and chromosomal alignment. Moreover, CCDC74A/B form homodimers in vivo, and their self-association activity is necessary for microtubule bundling and K-fiber formation. Conclusions We characterize CCDC74A and CCDC74B as microtubule-associated proteins that localize to spindles and are important K-fiber crosslinkers required for bipolar spindle formation and chromosome alignment. |
topic |
CCDC74A CCDC74B Kinetochore fiber Microtubule dynamic Spindle assembly Chromosomal alignment |
url |
http://link.springer.com/article/10.1186/s12915-019-0694-9 |
work_keys_str_mv |
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