miRNA-199a-5p functions as a tumor suppressor in prolactinomas
Prolactinomas are the most frequently observed pituitary adenomas (PAs), and 5%–18% tumors were resistant to the dopamine agonists (DAs). MicroRNAs (miRNAs) dysfunction play a key role in tumorigenesis. Agilent miRNA and an expression chip were used for six prolactinomas and three normal pituitary s...
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Online Access: | https://doi.org/10.1515/chem-2019-0036 |
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doaj-eb9ef3db1c5344599691f6b8aed46fba2021-09-06T19:19:35ZengDe GruyterOpen Chemistry2391-54202019-07-0117150651510.1515/chem-2019-0036chem-2019-0036miRNA-199a-5p functions as a tumor suppressor in prolactinomasJichao Wang0Jing Guo1Fei Wang2Lei Cao3Qian Liu4Jie Feng5Hongyun Wang6Hua Gao7Yazhuo Zhang8Beijing Neurosurgical Institute, Capital Medical University, Beijing, ChinaBeijing Neurosurgical Institute, Capital Medical University, Beijing, ChinaDepartment of Neurosurgery, Provincial Hospital Affiliated to Anhui Medical University, Hefei, Anhui Province, ChinaBeijing Neurosurgical Institute, Capital Medical University, Beijing, ChinaBeijing Neurosurgical Institute, Capital Medical University, Beijing, ChinaBeijing Neurosurgical Institute, Capital Medical University, Beijing, ChinaBeijing Neurosurgical Institute, Capital Medical University, Beijing, ChinaBeijing Neurosurgical Institute, Capital Medical University, Beijing, ChinaBeijing Neurosurgical Institute, Capital Medical University, Beijing, ChinaProlactinomas are the most frequently observed pituitary adenomas (PAs), and 5%–18% tumors were resistant to the dopamine agonists (DAs). MicroRNAs (miRNAs) dysfunction play a key role in tumorigenesis. Agilent miRNA and an expression chip were used for six prolactinomas and three normal pituitary specimens. Differentially expressed genes were confirmed by RT-qPCR. The level of DDR1 and SAT1 was determined with tissue micro-array (TMA) and western blot. A MMQ cell line was used for functional experiments. We have identified 5-miRNA and 12 target gene signatures of prolactinomas through gene ontology analysis. miRNA-199a-5p was selected for experiments that integrated the results from prolactinomas specimens and a rat prolactinoma model induced by 17-b-estradiol. Tumors with low miRNA-199a-5p had a significantly invasive behavior and a higher tumor volume (p<0.05). DDR1 and SAT1, target genes of miRNA-199a-5p, had higher H-scores in the invasive group than those of the non-invasive group through TMA. An overexpression of miRNA-119a-5p suppressed the PRL secretion and the cell viability through upregulated the apoptosis level in MMQ cells (p<0.01). Furthermore, we found the target genes expression of DDR1 and SAT1 were affected by miRNA-199a-5p regardless of mRNA levels or protein levels. This study provided evidence that downregulation of miRNA-199a-5p may contribute to prolactinoma tumorigenesis.https://doi.org/10.1515/chem-2019-0036prolactinomasmirna-199a-5ptumorigenesisddr1sat1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jichao Wang Jing Guo Fei Wang Lei Cao Qian Liu Jie Feng Hongyun Wang Hua Gao Yazhuo Zhang |
spellingShingle |
Jichao Wang Jing Guo Fei Wang Lei Cao Qian Liu Jie Feng Hongyun Wang Hua Gao Yazhuo Zhang miRNA-199a-5p functions as a tumor suppressor in prolactinomas Open Chemistry prolactinomas mirna-199a-5p tumorigenesis ddr1 sat1 |
author_facet |
Jichao Wang Jing Guo Fei Wang Lei Cao Qian Liu Jie Feng Hongyun Wang Hua Gao Yazhuo Zhang |
author_sort |
Jichao Wang |
title |
miRNA-199a-5p functions as a tumor suppressor in prolactinomas |
title_short |
miRNA-199a-5p functions as a tumor suppressor in prolactinomas |
title_full |
miRNA-199a-5p functions as a tumor suppressor in prolactinomas |
title_fullStr |
miRNA-199a-5p functions as a tumor suppressor in prolactinomas |
title_full_unstemmed |
miRNA-199a-5p functions as a tumor suppressor in prolactinomas |
title_sort |
mirna-199a-5p functions as a tumor suppressor in prolactinomas |
publisher |
De Gruyter |
series |
Open Chemistry |
issn |
2391-5420 |
publishDate |
2019-07-01 |
description |
Prolactinomas are the most frequently observed pituitary adenomas (PAs), and 5%–18% tumors were resistant to the dopamine agonists (DAs). MicroRNAs (miRNAs) dysfunction play a key role in tumorigenesis. Agilent miRNA and an expression chip were used for six prolactinomas and three normal pituitary specimens. Differentially expressed genes were confirmed by RT-qPCR. The level of DDR1 and SAT1 was determined with tissue micro-array (TMA) and western blot. A MMQ cell line was used for functional experiments. We have identified 5-miRNA and 12 target gene signatures of prolactinomas through gene ontology analysis. miRNA-199a-5p was selected for experiments that integrated the results from prolactinomas specimens and a rat prolactinoma model induced by 17-b-estradiol. Tumors with low miRNA-199a-5p had a significantly invasive behavior and a higher tumor volume (p<0.05). DDR1 and SAT1, target genes of miRNA-199a-5p, had higher H-scores in the invasive group than those of the non-invasive group through TMA. An overexpression of miRNA-119a-5p suppressed the PRL secretion and the cell viability through upregulated the apoptosis level in MMQ cells (p<0.01). Furthermore, we found the target genes expression of DDR1 and SAT1 were affected by miRNA-199a-5p regardless of mRNA levels or protein levels. This study provided evidence that downregulation of miRNA-199a-5p may contribute to prolactinoma tumorigenesis. |
topic |
prolactinomas mirna-199a-5p tumorigenesis ddr1 sat1 |
url |
https://doi.org/10.1515/chem-2019-0036 |
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