Oxytocin Receptor Exon III Methylation in the Umbilical Cord Blood of Newborns With Prenatal Exposure to Crack Cocaine
BackgroundPrenatal cocaine exposure (PCE) is associated with behavioral, cognitive, and social consequences in children that might persist into later development. However, there are still few data concerning epigenetic mechanisms associated with the effects of gestational cocaine exposure, particula...
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Frontiers Media S.A.
2021-06-01
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Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.639287/full |
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language |
English |
format |
Article |
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DOAJ |
author |
Talita Baptista Lucas Araújo de Azeredo Aline Zaparte Thiago Wendt Viola Sayra Catalina Coral Maria Aparecida Nagai Maria Aparecida Nagai Flávia Rotea Mangone Ana Carolina Pavanelli Jaqueline B. Schuch Victor Mardini Victor Mardini Claudia M. Szobot Claudia M. Szobot Rodrigo Grassi-Oliveira Rodrigo Grassi-Oliveira |
spellingShingle |
Talita Baptista Lucas Araújo de Azeredo Aline Zaparte Thiago Wendt Viola Sayra Catalina Coral Maria Aparecida Nagai Maria Aparecida Nagai Flávia Rotea Mangone Ana Carolina Pavanelli Jaqueline B. Schuch Victor Mardini Victor Mardini Claudia M. Szobot Claudia M. Szobot Rodrigo Grassi-Oliveira Rodrigo Grassi-Oliveira Oxytocin Receptor Exon III Methylation in the Umbilical Cord Blood of Newborns With Prenatal Exposure to Crack Cocaine Frontiers in Cell and Developmental Biology cocaine substance use disorders OXTR epigenetic prenatal pregnancy |
author_facet |
Talita Baptista Lucas Araújo de Azeredo Aline Zaparte Thiago Wendt Viola Sayra Catalina Coral Maria Aparecida Nagai Maria Aparecida Nagai Flávia Rotea Mangone Ana Carolina Pavanelli Jaqueline B. Schuch Victor Mardini Victor Mardini Claudia M. Szobot Claudia M. Szobot Rodrigo Grassi-Oliveira Rodrigo Grassi-Oliveira |
author_sort |
Talita Baptista |
title |
Oxytocin Receptor Exon III Methylation in the Umbilical Cord Blood of Newborns With Prenatal Exposure to Crack Cocaine |
title_short |
Oxytocin Receptor Exon III Methylation in the Umbilical Cord Blood of Newborns With Prenatal Exposure to Crack Cocaine |
title_full |
Oxytocin Receptor Exon III Methylation in the Umbilical Cord Blood of Newborns With Prenatal Exposure to Crack Cocaine |
title_fullStr |
Oxytocin Receptor Exon III Methylation in the Umbilical Cord Blood of Newborns With Prenatal Exposure to Crack Cocaine |
title_full_unstemmed |
Oxytocin Receptor Exon III Methylation in the Umbilical Cord Blood of Newborns With Prenatal Exposure to Crack Cocaine |
title_sort |
oxytocin receptor exon iii methylation in the umbilical cord blood of newborns with prenatal exposure to crack cocaine |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2021-06-01 |
description |
BackgroundPrenatal cocaine exposure (PCE) is associated with behavioral, cognitive, and social consequences in children that might persist into later development. However, there are still few data concerning epigenetic mechanisms associated with the effects of gestational cocaine exposure, particularly in human newborns.AimsWe investigated the effects of PCE on DNA methylation patterns of the Oxytocin Receptor (OXTR) gene in the umbilical cord blood (UCB). The relationship between UCB DNA methylation levels and the severity of the mother’s cocaine use during pregnancy was also evaluated.MethodsIn this cross-sectional study, 28 UCB samples of newborns with a history of crack cocaine exposure in utero and 30 UCB samples of non-exposed newborns (NEC) were compared for DNA methylation levels at two genomic loci located in exon III of the OXTR gene (OXTR1 and OXTR2) through pyrosequencing. Maternal psychopathology was investigated using the Mini International Neuropsychiatric Interview, and substance use characteristics and addiction severity were assessed using the Smoking and Substance Involvement Screening Test (ASSIST).ResultsNo differences between newborns with a history of PCE and NEC were observed in OXTR1 or OXTR2 DNA methylation levels. However, regression analyses showed that maternal addiction severity for crack cocaine use predicted OXTR1 DNA methylation in newborns.ConclusionThese data suggest that OXTR methylation levels in the UCB of children are affected by the severity of maternal crack cocaine usage. Larger studies are likely to detect specific changes in DNA methylation relevant to the consequences of PCE. |
topic |
cocaine substance use disorders OXTR epigenetic prenatal pregnancy |
url |
https://www.frontiersin.org/articles/10.3389/fcell.2021.639287/full |
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doaj-eba555c37b8f4611a11a3589e22eb9102021-06-04T08:35:54ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-06-01910.3389/fcell.2021.639287639287Oxytocin Receptor Exon III Methylation in the Umbilical Cord Blood of Newborns With Prenatal Exposure to Crack CocaineTalita Baptista0Lucas Araújo de Azeredo1Aline Zaparte2Thiago Wendt Viola3Sayra Catalina Coral4Maria Aparecida Nagai5Maria Aparecida Nagai6Flávia Rotea Mangone7Ana Carolina Pavanelli8Jaqueline B. Schuch9Victor Mardini10Victor Mardini11Claudia M. Szobot12Claudia M. Szobot13Rodrigo Grassi-Oliveira14Rodrigo Grassi-Oliveira15Developmental Cognitive Neuroscience Lab, School of Medicine, Brain Institute of the Rio Grande do Sul (InsCer), Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, BrazilDevelopmental Cognitive Neuroscience Lab, School of Medicine, Brain Institute of the Rio Grande do Sul (InsCer), Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, BrazilDevelopmental Cognitive Neuroscience Lab, School of Medicine, Brain Institute of the Rio Grande do Sul (InsCer), Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, BrazilDevelopmental Cognitive Neuroscience Lab, School of Medicine, Brain Institute of the Rio Grande do Sul (InsCer), Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, BrazilDevelopmental Cognitive Neuroscience Lab, School of Medicine, Brain Institute of the Rio Grande do Sul (InsCer), Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, BrazilDiscipline of Oncology, Department of Radiology and Oncology, Faculty of Medicine, University of São Paulo, São Paulo, BrazilLaboratory of Molecular Genetics, Center for Translational Research in Oncology, Cancer Institute of the State of São Paulo, São Paulo, BrazilLaboratory of Molecular Genetics, Center for Translational Research in Oncology, Cancer Institute of the State of São Paulo, São Paulo, BrazilLaboratory of Molecular Genetics, Center for Translational Research in Oncology, Cancer Institute of the State of São Paulo, São Paulo, BrazilGraduate Program in Psychiatry and Behavioral Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, BrazilGraduate Program in Psychiatry and Behavioral Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, BrazilChild and Adolescent Psychiatry Service (SPIA), Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, BrazilGraduate Program in Psychiatry and Behavioral Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, BrazilChild and Adolescent Psychiatry Service (SPIA), Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, BrazilDevelopmental Cognitive Neuroscience Lab, School of Medicine, Brain Institute of the Rio Grande do Sul (InsCer), Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, BrazilTranslational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Aarhus, DenmarkBackgroundPrenatal cocaine exposure (PCE) is associated with behavioral, cognitive, and social consequences in children that might persist into later development. However, there are still few data concerning epigenetic mechanisms associated with the effects of gestational cocaine exposure, particularly in human newborns.AimsWe investigated the effects of PCE on DNA methylation patterns of the Oxytocin Receptor (OXTR) gene in the umbilical cord blood (UCB). The relationship between UCB DNA methylation levels and the severity of the mother’s cocaine use during pregnancy was also evaluated.MethodsIn this cross-sectional study, 28 UCB samples of newborns with a history of crack cocaine exposure in utero and 30 UCB samples of non-exposed newborns (NEC) were compared for DNA methylation levels at two genomic loci located in exon III of the OXTR gene (OXTR1 and OXTR2) through pyrosequencing. Maternal psychopathology was investigated using the Mini International Neuropsychiatric Interview, and substance use characteristics and addiction severity were assessed using the Smoking and Substance Involvement Screening Test (ASSIST).ResultsNo differences between newborns with a history of PCE and NEC were observed in OXTR1 or OXTR2 DNA methylation levels. However, regression analyses showed that maternal addiction severity for crack cocaine use predicted OXTR1 DNA methylation in newborns.ConclusionThese data suggest that OXTR methylation levels in the UCB of children are affected by the severity of maternal crack cocaine usage. Larger studies are likely to detect specific changes in DNA methylation relevant to the consequences of PCE.https://www.frontiersin.org/articles/10.3389/fcell.2021.639287/fullcocainesubstance use disordersOXTRepigeneticprenatalpregnancy |