Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN

Cystic echinococcosis (CE) is a globally parasitic zoonosis caused by larval stages of Echinococcus granulosus. This study investigated E. granulosus genotypes isolated from livestock and humans in the Golestan province, northern Iran, southeast of the Caspian sea, using partial sequencing data of t...

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Main Authors: Mitra SHARBATKHORI, Asal TANZIFI, Sima ROSTAMI, Masoomeh ROSTAMI, Majid FASIHI HARANDI
Format: Article
Language:English
Published: Universidade de São Paulo 2016-01-01
Series:Revista do Instituto de Medicina Tropical de São Paulo
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000228&lng=en&tlng=en
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spelling doaj-ebb42f10b62046c4ae0f655e509c496f2020-11-25T00:48:57ZengUniversidade de São PauloRevista do Instituto de Medicina Tropical de São Paulo1678-99462016-01-0158010.1590/S1678-9946201658038S0036-46652016005000228Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRANMitra SHARBATKHORIAsal TANZIFISima ROSTAMIMasoomeh ROSTAMIMajid FASIHI HARANDICystic echinococcosis (CE) is a globally parasitic zoonosis caused by larval stages of Echinococcus granulosus. This study investigated E. granulosus genotypes isolated from livestock and humans in the Golestan province, northern Iran, southeast of the Caspian sea, using partial sequencing data of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1) mitochondrial genes. Seventy E. granulosus isolates were collected from animals in slaughterhouses: 18 isolates from sheep, 40 from cattle, nine from camels, two from buffaloes and one from a goat, along with four human isolates (formalin-fixed, paraffin-embedded tissues) from CE patients of provincial hospitals. All isolates were successfully analysed by PCR amplification and sequencing. The sequence analysis found four E. granulosus genotypes among the 74 CE isolates: G1 (78.3%), G2 (2.7%), G3 (15%) and G6 (4%). The G1-G3 complex genotype was found in all of the sheep, goat, cattle and buffalo isolates. Among the nine camel isolates, the frequency of G1-G3 and G6 genotypes were 66.7% and 33.3%, respectively. All four human CE isolates belonged to E. granulosus sensu stricto. This study reports the first occurrence of the G2 genotype in cattle from Iran and confirms the previously reported G3 genotype in camels in the same country.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000228&lng=en&tlng=enEchinococcus granulosusGenotypingcox1nad1Iran
collection DOAJ
language English
format Article
sources DOAJ
author Mitra SHARBATKHORI
Asal TANZIFI
Sima ROSTAMI
Masoomeh ROSTAMI
Majid FASIHI HARANDI
spellingShingle Mitra SHARBATKHORI
Asal TANZIFI
Sima ROSTAMI
Masoomeh ROSTAMI
Majid FASIHI HARANDI
Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN
Revista do Instituto de Medicina Tropical de São Paulo
Echinococcus granulosus
Genotyping
cox1
nad1
Iran
author_facet Mitra SHARBATKHORI
Asal TANZIFI
Sima ROSTAMI
Masoomeh ROSTAMI
Majid FASIHI HARANDI
author_sort Mitra SHARBATKHORI
title Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN
title_short Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN
title_full Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN
title_fullStr Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN
title_full_unstemmed Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN
title_sort echinococcus granulosus sensu lato genotypes in domestic livestock and humans in golestan province, iran
publisher Universidade de São Paulo
series Revista do Instituto de Medicina Tropical de São Paulo
issn 1678-9946
publishDate 2016-01-01
description Cystic echinococcosis (CE) is a globally parasitic zoonosis caused by larval stages of Echinococcus granulosus. This study investigated E. granulosus genotypes isolated from livestock and humans in the Golestan province, northern Iran, southeast of the Caspian sea, using partial sequencing data of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1) mitochondrial genes. Seventy E. granulosus isolates were collected from animals in slaughterhouses: 18 isolates from sheep, 40 from cattle, nine from camels, two from buffaloes and one from a goat, along with four human isolates (formalin-fixed, paraffin-embedded tissues) from CE patients of provincial hospitals. All isolates were successfully analysed by PCR amplification and sequencing. The sequence analysis found four E. granulosus genotypes among the 74 CE isolates: G1 (78.3%), G2 (2.7%), G3 (15%) and G6 (4%). The G1-G3 complex genotype was found in all of the sheep, goat, cattle and buffalo isolates. Among the nine camel isolates, the frequency of G1-G3 and G6 genotypes were 66.7% and 33.3%, respectively. All four human CE isolates belonged to E. granulosus sensu stricto. This study reports the first occurrence of the G2 genotype in cattle from Iran and confirms the previously reported G3 genotype in camels in the same country.
topic Echinococcus granulosus
Genotyping
cox1
nad1
Iran
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000228&lng=en&tlng=en
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AT simarostami echinococcusgranulosussensulatogenotypesindomesticlivestockandhumansingolestanprovinceiran
AT masoomehrostami echinococcusgranulosussensulatogenotypesindomesticlivestockandhumansingolestanprovinceiran
AT majidfasihiharandi echinococcusgranulosussensulatogenotypesindomesticlivestockandhumansingolestanprovinceiran
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