A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats
170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per secon...
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doaj-ebe4394b50a94ac9acb5b66b8ad7e20c2020-11-24T21:19:57ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882014-01-01201410.1155/2014/962343962343A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO RatsDing Luo0Xiaonong Fan1Congcong Ma2Tongtao Fan3Xiaoguang Wang4Nvzi Chang5Lingxin Li6Yanan Zhang7Zhihong Meng8Shu Wang9Xuemin Shi10Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaThe Acupuncture & Moxibustion Institute, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaPostgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaPostgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaPostgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaPostgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaPostgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaThe Acupuncture & Moxibustion Institute, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaTianjin Key Laboratory of Acupuncture & Moxibustion Science, Tianjin 300193, ChinaThe Acupuncture & Moxibustion Institute, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaThe Acupuncture & Moxibustion Institute, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin+, BrdU+ and nestin/BrdU+) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat’s “self-healing” capacity 3 days after MCAO. And the “self-healing” capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.http://dx.doi.org/10.1155/2014/962343 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ding Luo Xiaonong Fan Congcong Ma Tongtao Fan Xiaoguang Wang Nvzi Chang Lingxin Li Yanan Zhang Zhihong Meng Shu Wang Xuemin Shi |
spellingShingle |
Ding Luo Xiaonong Fan Congcong Ma Tongtao Fan Xiaoguang Wang Nvzi Chang Lingxin Li Yanan Zhang Zhihong Meng Shu Wang Xuemin Shi A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats Evidence-Based Complementary and Alternative Medicine |
author_facet |
Ding Luo Xiaonong Fan Congcong Ma Tongtao Fan Xiaoguang Wang Nvzi Chang Lingxin Li Yanan Zhang Zhihong Meng Shu Wang Xuemin Shi |
author_sort |
Ding Luo |
title |
A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats |
title_short |
A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats |
title_full |
A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats |
title_fullStr |
A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats |
title_full_unstemmed |
A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats |
title_sort |
study on the effect of neurogenesis and regulation of gsk3β/pp2a expression in acupuncture treatment of neural functional damage caused by focal ischemia in mcao rats |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2014-01-01 |
description |
170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin+, BrdU+ and nestin/BrdU+) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat’s “self-healing” capacity 3 days after MCAO. And the “self-healing” capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats. |
url |
http://dx.doi.org/10.1155/2014/962343 |
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