A Mathematical Analysis of RNA Structural Motifs in Viruses

• Main Authors: Alexander Churkin, Franziska Totzeck, Rami Zakh, Marina Parr, Tamir Tuller, Dmitrij Frishman, Danny Barash
• Format: Article
• Language: English
• Published: MDPI AG 2021-03-01
• Series: Mathematics
• Subjects: RNA graph representation; Laplacian eigenvalues; topological indices; folding energy; mutational robustness
• Online Access: https://www.mdpi.com/2227-7390/9/6/585

RNA stem-loop structures play an important role in almost every step of the viral replication cycle. In this contribution, a mathematical analysis is performed on a large dataset of RNA secondary structure elements in the coding regions of viruses by using topological indices that capture the Laplacian eigenvalues of the associated RNA graph representations and thereby enable structural classification, supplemented by folding energy and mutational robustness. The application of such an analysis for viral RNA structural motifs is described, being able to extract structural categories such as stem-loop structures of different sizes according to the tree-graph representation of the RNA structure, in our attempt to find novel functional motifs. While the analysis is carried on a large dataset of viral RNA structures, it can be applied more generally to other data that involve RNA secondary structures in biological agents.