Up-regulation of TLR2 and TLR4 in high mobility group Box1-stimulated macrophages in pulpitis patients

• Main Authors: Javad Mahmoudi, Babak Sabermarouf, Behzad Baradaran, Leila Sadat-Hatamnezhad, Siamak Sandoghchian Shotorbani
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2017-02-01
• Series: Iranian Journal of Basic Medical Sciences
• Subjects: HMGB1; Pulpitis; TLR2; TLR4
• Online Access: http://ijbms.mums.ac.ir/article_8250_3b98ce9154b9aecf3ce2d86fb6b01e14.pdf

Objective(s): High Mobility Group Box1 (HMGB1) is a nonhistone, DNA-binding protein that serves a crucial role in regulating gene transcription and is involved in a variety of proinflammatory, extracellular activities. The aim of this study was to explore whether HMGB1 stimulation can up-regulate the expression of Toll-like Receptor 2 (TLR2) and Toll-like Receptor 4 (TLR4) on macrophages from pulpitis and to clarify the subsequent events involving Th17 cells and Th17 cell-associated cytokine changes. Materials and Methods: Having prepared dental pulp tissues of pulpitis and healthy controls, macrophage were isolated and cultured. Macrophages were thereafter stimulated by HMGB1 time course. RT-QPCR, flowcytometer, immunofluorescence, Western blotting, and ELISA techniques were used in the present research. Results: Our results showed that the expression of TLR2 and TLR4 on macrophages stimulated with HMGB1 increased in pulpitis compared with controls (macrophages without HMGB1 stimulation) with a statistical significance (P