Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure
Concurrent with rising production of carbon-based engineered nanomaterials is a potential increase in respiratory and cardiovascular diseases due to exposure to nanomaterials in the workplace atmosphere. While single-cell models of pulmonary exposure are often used to determine the potential toxicit...
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doaj-ebf1cd1c6019406c9eee44c5d9c87e632020-11-24T21:35:43ZengElsevierToxicology Reports2214-75002019-01-016482488Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposureBrandi N. Snyder-Talkington0Chunlin Dong1Vincent Castranova2Yong Qian3Nancy L. Guo4West Virginia University Cancer Institute, West Virginia University, Morgantown, WV, 26506, United StatesWest Virginia University Cancer Institute, West Virginia University, Morgantown, WV, 26506, United StatesDepartment of Pharmaceutical Sciences, School of Pharmacy, West Virginia University, Morgantown, WV, 26506, United StatesNational Institute for Occupational and Environmental Safety and Health, 1095 Willowdale Rd., Morgantown, WV, 26505, United StatesWest Virginia University Cancer Institute, West Virginia University, Morgantown, WV, 26506, United States; Department of Occupational and Environmental Health Sciences, School of Public Health, West Virginia University, Morgantown, WV, 26506, United States; Corresponding author at: West Virginia University Cancer Institute, West Virginia University, Morgantown, WV, 26506-9300, United States.Concurrent with rising production of carbon-based engineered nanomaterials is a potential increase in respiratory and cardiovascular diseases due to exposure to nanomaterials in the workplace atmosphere. While single-cell models of pulmonary exposure are often used to determine the potential toxicity of nanomaterials in vitro, previous studies have shown that coculture cell models better represent the cellular response and crosstalk that occurs in vivo. This study identified differential gene regulation in human small airway epithelial cells (SAECs) grown either in monoculture or in coculture with human microvascular endothelial cells following exposure of the SAECs to multiwalled carbon nanotubes (MWCNTs). SAEC genes that either changed their regulation direction from upregulated in monoculture to downregulated in coculture (or vice versa) or had a more than a two-fold changed in the same regulation direction were identified. Genes that changed regulation direction were most often involved in the processes of cellular growth and proliferation and cellular immune response and inflammation. Genes that had a more than a two-fold change in regulation in the same direction were most often involved in the inflammatory response. The direction and fold-change of this differential gene regulation suggests that toxicity testing in monoculture may exaggerate cellular responses to MWCNTs, and coculture of cells may provide a more in-depth assessment of toxicological responses. Keywords: Multiwalled carbon nanotubes, MWCNT, Coculture, Pulmonary exposure, Gene regulationhttp://www.sciencedirect.com/science/article/pii/S2214750018304384 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Brandi N. Snyder-Talkington Chunlin Dong Vincent Castranova Yong Qian Nancy L. Guo |
spellingShingle |
Brandi N. Snyder-Talkington Chunlin Dong Vincent Castranova Yong Qian Nancy L. Guo Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure Toxicology Reports |
author_facet |
Brandi N. Snyder-Talkington Chunlin Dong Vincent Castranova Yong Qian Nancy L. Guo |
author_sort |
Brandi N. Snyder-Talkington |
title |
Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure |
title_short |
Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure |
title_full |
Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure |
title_fullStr |
Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure |
title_full_unstemmed |
Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure |
title_sort |
differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure |
publisher |
Elsevier |
series |
Toxicology Reports |
issn |
2214-7500 |
publishDate |
2019-01-01 |
description |
Concurrent with rising production of carbon-based engineered nanomaterials is a potential increase in respiratory and cardiovascular diseases due to exposure to nanomaterials in the workplace atmosphere. While single-cell models of pulmonary exposure are often used to determine the potential toxicity of nanomaterials in vitro, previous studies have shown that coculture cell models better represent the cellular response and crosstalk that occurs in vivo. This study identified differential gene regulation in human small airway epithelial cells (SAECs) grown either in monoculture or in coculture with human microvascular endothelial cells following exposure of the SAECs to multiwalled carbon nanotubes (MWCNTs). SAEC genes that either changed their regulation direction from upregulated in monoculture to downregulated in coculture (or vice versa) or had a more than a two-fold changed in the same regulation direction were identified. Genes that changed regulation direction were most often involved in the processes of cellular growth and proliferation and cellular immune response and inflammation. Genes that had a more than a two-fold change in regulation in the same direction were most often involved in the inflammatory response. The direction and fold-change of this differential gene regulation suggests that toxicity testing in monoculture may exaggerate cellular responses to MWCNTs, and coculture of cells may provide a more in-depth assessment of toxicological responses. Keywords: Multiwalled carbon nanotubes, MWCNT, Coculture, Pulmonary exposure, Gene regulation |
url |
http://www.sciencedirect.com/science/article/pii/S2214750018304384 |
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