A New Bioartificial Liver Using Porcine Hepatocyte Spheroids in High-Cell-Density Suspension Perfusion Culture: In Vitro Performance in Synthesized Culture Medium and in 100% Human Plasma
A prototype of a bioartificial liver (BAL) based on suspension perfusion culture of porcine hepatocyte spheroids was developed at 150 ml scale. About 2% (4 × 10 9 cells) of whole human liver cells was immobilized. The cell density in the bioreactor was 2.7 × 10 7 cells/ml, which was almost comparabl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
1999-09-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/096368979900800508 |