A New Bioartificial Liver Using Porcine Hepatocyte Spheroids in High-Cell-Density Suspension Perfusion Culture: In Vitro Performance in Synthesized Culture Medium and in 100% Human Plasma
A prototype of a bioartificial liver (BAL) based on suspension perfusion culture of porcine hepatocyte spheroids was developed at 150 ml scale. About 2% (4 × 10 9 cells) of whole human liver cells was immobilized. The cell density in the bioreactor was 2.7 × 10 7 cells/ml, which was almost comparabl...
Main Authors: | Yasuyuki Sakai, Katsutoshi Naruse, Ikuo Nagashima, Tetsuichiro Muto, Motoyuki Suzuki |
---|---|
Format: | Article |
Language: | English |
Published: |
SAGE Publishing
1999-09-01
|
Series: | Cell Transplantation |
Online Access: | https://doi.org/10.1177/096368979900800508 |
Similar Items
-
Short-Term Hypothermic Preservation of Porcine Hepatocyte Spheroids using uw Solution
by: Yasuyuki Sakai, et al.
Published: (1996-07-01) -
Formation of Porcine Hepatocyte Spheroids for use in a Bioartificial Liver
by: Arye Lazar, et al.
Published: (1995-05-01) -
Characterisation of primary porcine hepatocyte culture systems for use in bioartificial liver devices
by: Nelson, Leonard Joseph
Published: (2002) -
Utilize biomaterials to culture hepatocytes for development of bioartificial liver
by: 楊靜仁
Published: (2002) -
Functional Evaluation of a Bioartificial Liver Support System Using Immobilized Hepatocyte Spheroids in a Porcine Model of Acute Liver Failure
by: Ji-Hyun Lee, et al.
Published: (2017-06-01)