Extraction of Teucrium manghuaense and Evaluation of the Bioactivity of Its Extract

• Main Authors: Mingyue Wang, Mingjun He, Huicai Zeng, Guihao Yin
• Format: Article
• Language: English
• Published: MDPI AG 2009-10-01
• Series: International Journal of Molecular Sciences
• Subjects: Teucrium manghuaense; extraction; squalene; GC-MS; antioxidant; anti-tumor
• Online Access: http://www.mdpi.com/1422-0067/10/10/4330/

The ethanol extract of Teucrium manghuaense grown in Hainan province (China) was analysed by GC and GC/MS. Of the constituents 84–96% were identified on the basis of their GC retention times and their mass spectra in regard to authentic compounds. The results revealed that it contained 9-methyl-9-azabicyclo[4.2.1]nonane (7.43%), 2-methylpyrrolidine (19.42%), 3,7,11,15-tetramethyl-2-hexadecen-1-ol (10.84%), and squalene (28.55%), as major components, constituting 66.24% of the extract. The chemical characterization of the ethanol extract by GC/MS also allowed identification of 1-octen-3-one (3.41%), 2-pentyl-piperidine (2.25%), 1-(2-methyl-1-propenyl)-piperidine (4.63%), 2,2'-diethoxy-5,5'-bi-1-pyrroline (0.41%), (Z,Z,Z)-9,12,15-octadecatrieniic acid, 2,3-dihydroxypropyl ester (1.56%), vitamin E (2.95%) and stigmasterol (1.39%). Finally, the antioxidant and anti-tumor activities of the ethanol extract have been evaluated. Results show that the extract of Teucrium manghuaense leaf possesses strong DPPH-, hydroxyl radical scavenging activity and anti-tumor activity.