Influence of Schistosoma japonicum programmed cell death protein 10 on the growth and development of schistosomula
Abstract Background Schistosomiasis caused by Schistosoma japonicum is among the most serious endemic zoonoses in China. To study interactions between schistosomula, the pre-adult juvenile stage, and hosts, it is important to study the functions of key genes involved in schistosomula growth and deve...
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doaj-ec18704a1a7e4d08942081b78d96bcda2020-11-24T21:59:44ZengBMCParasites & Vectors1756-33052018-01-0111111110.1186/s13071-018-2636-8Influence of Schistosoma japonicum programmed cell death protein 10 on the growth and development of schistosomulaYan Ru Gao0Wen Ling Huang1Chun Lian Tang2Rong Liu3Qin Ping Zhao4Zhen Ping Ming5Hui Fen Dong6Hubei Province Key Laboratory of Allergy and Immunology, Department of Parasitology, Wuhan University School of Basic Medical SciencesHubei Province Key Laboratory of Allergy and Immunology, Department of Parasitology, Wuhan University School of Basic Medical SciencesDepartment of Clinical Laboratory, Wuchang HospitalHubei Province Key Laboratory of Allergy and Immunology, Department of Parasitology, Wuhan University School of Basic Medical SciencesHubei Province Key Laboratory of Allergy and Immunology, Department of Parasitology, Wuhan University School of Basic Medical SciencesHubei Province Key Laboratory of Allergy and Immunology, Department of Parasitology, Wuhan University School of Basic Medical SciencesHubei Province Key Laboratory of Allergy and Immunology, Department of Parasitology, Wuhan University School of Basic Medical SciencesAbstract Background Schistosomiasis caused by Schistosoma japonicum is among the most serious endemic zoonoses in China. To study interactions between schistosomula, the pre-adult juvenile stage, and hosts, it is important to study the functions of key genes involved in schistosomula growth and development. Programmed cell death protein 10 (pcdp10) is an important apoptosis-related gene with various biological functions. This study described the molecular characterization of S. japonicum PCDP10 (SjPCDP10) and evaluated its functions in schistosomula. Methods Real-time quantitative polymerase chain reaction (qPCR) and western blot were used to detect Sjpcdp10 mRNA and protein levels, respectively, at different developmental stages. Immunolocalization was performed to determine SjPCDP10 expression in the parasite. RNA interference (RNAi) experiments were used to assess gene functions associated with SjPCDP10 in schistosomula growth and development. Results Real-time qPCR revealed that Sjpcdp10 was expressed during all investigated developmental stages and upregulated during schistosomula growth and development. Histochemical localization showed that SjPCDP10 was mainly distributed in the teguments of schistosomula in all investigated stages and part of the parenchymal area of 14-, 18-, and 21-day-old schistosomula. Following Sjpcdp10 knockdown by RNAi, the lengths, widths, areas, and volumes of schistosomula were significantly lower than those in the control group. Scanning electron microscopy showed that the body surfaces of schistosomula subjected to RNAi were seriously damaged, with few tegumental spines and sensory papillae. Transmission electron microscopy indicated that the teguments of Sjpcdp10-knockdown schistosomula were incomplete, the number of layers was reduced, and the thickness decreased significantly as compared with those in the control group. Furthermore, terminal deoxynucleotidyl transferase dUTP nick-end labelling results showed that the rate of apoptosis in Sjpcdp10-knockdown schistosomula was significantly higher than that in the control group. Conclusions Sjpcdp10-knockdown influenced the growth and development of schistosomula. Therefore, our results indicated that SjPCDP10 contributes to the regulation of cell apoptosis and is essential for schistosomula growth and development.http://link.springer.com/article/10.1186/s13071-018-2636-8Schistosoma japonicumSchistosomulaProgrammed cell death factor-10Growth and developmentTegumentApoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yan Ru Gao Wen Ling Huang Chun Lian Tang Rong Liu Qin Ping Zhao Zhen Ping Ming Hui Fen Dong |
spellingShingle |
Yan Ru Gao Wen Ling Huang Chun Lian Tang Rong Liu Qin Ping Zhao Zhen Ping Ming Hui Fen Dong Influence of Schistosoma japonicum programmed cell death protein 10 on the growth and development of schistosomula Parasites & Vectors Schistosoma japonicum Schistosomula Programmed cell death factor-10 Growth and development Tegument Apoptosis |
author_facet |
Yan Ru Gao Wen Ling Huang Chun Lian Tang Rong Liu Qin Ping Zhao Zhen Ping Ming Hui Fen Dong |
author_sort |
Yan Ru Gao |
title |
Influence of Schistosoma japonicum programmed cell death protein 10 on the growth and development of schistosomula |
title_short |
Influence of Schistosoma japonicum programmed cell death protein 10 on the growth and development of schistosomula |
title_full |
Influence of Schistosoma japonicum programmed cell death protein 10 on the growth and development of schistosomula |
title_fullStr |
Influence of Schistosoma japonicum programmed cell death protein 10 on the growth and development of schistosomula |
title_full_unstemmed |
Influence of Schistosoma japonicum programmed cell death protein 10 on the growth and development of schistosomula |
title_sort |
influence of schistosoma japonicum programmed cell death protein 10 on the growth and development of schistosomula |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2018-01-01 |
description |
Abstract Background Schistosomiasis caused by Schistosoma japonicum is among the most serious endemic zoonoses in China. To study interactions between schistosomula, the pre-adult juvenile stage, and hosts, it is important to study the functions of key genes involved in schistosomula growth and development. Programmed cell death protein 10 (pcdp10) is an important apoptosis-related gene with various biological functions. This study described the molecular characterization of S. japonicum PCDP10 (SjPCDP10) and evaluated its functions in schistosomula. Methods Real-time quantitative polymerase chain reaction (qPCR) and western blot were used to detect Sjpcdp10 mRNA and protein levels, respectively, at different developmental stages. Immunolocalization was performed to determine SjPCDP10 expression in the parasite. RNA interference (RNAi) experiments were used to assess gene functions associated with SjPCDP10 in schistosomula growth and development. Results Real-time qPCR revealed that Sjpcdp10 was expressed during all investigated developmental stages and upregulated during schistosomula growth and development. Histochemical localization showed that SjPCDP10 was mainly distributed in the teguments of schistosomula in all investigated stages and part of the parenchymal area of 14-, 18-, and 21-day-old schistosomula. Following Sjpcdp10 knockdown by RNAi, the lengths, widths, areas, and volumes of schistosomula were significantly lower than those in the control group. Scanning electron microscopy showed that the body surfaces of schistosomula subjected to RNAi were seriously damaged, with few tegumental spines and sensory papillae. Transmission electron microscopy indicated that the teguments of Sjpcdp10-knockdown schistosomula were incomplete, the number of layers was reduced, and the thickness decreased significantly as compared with those in the control group. Furthermore, terminal deoxynucleotidyl transferase dUTP nick-end labelling results showed that the rate of apoptosis in Sjpcdp10-knockdown schistosomula was significantly higher than that in the control group. Conclusions Sjpcdp10-knockdown influenced the growth and development of schistosomula. Therefore, our results indicated that SjPCDP10 contributes to the regulation of cell apoptosis and is essential for schistosomula growth and development. |
topic |
Schistosoma japonicum Schistosomula Programmed cell death factor-10 Growth and development Tegument Apoptosis |
url |
http://link.springer.com/article/10.1186/s13071-018-2636-8 |
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