Non thermal effects of radiofrequency electromagnetic field exposure on neural cells

Non thermal effects of radiofrequency electromagnetic field exposure on neural cells

The non-thermal mechanisms, underlying the damage induced on human cells by radiofrequency electromagnetic fields (RF-EMFs), are still unclear and only few studies reported about the effect of RFEMFs on self-renewal of neural progenitor cells. In this research, we investigated the influence of low-i...

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Main Authors: Grasso Rosaria, Pellitteri Rosalia, Caravella Santi Armando, Musumeci Francesco, Raciti Giuseppina, Scordino Agata, Sposito Giovanni, Triglia Antonio, Campisi Agata
Format: Article
Language:English
Published: EDP Sciences 2020-01-01
Series:EPJ Web of Conferences
Online Access:https://www.epj-conferences.org/articles/epjconf/pdf/2020/06/epjconf_fismat2019_00004.pdf
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spelling doaj-ec1aba7befc643cdb16878a649d693b02021-08-02T15:47:19ZengEDP SciencesEPJ Web of Conferences2100-014X2020-01-012300000410.1051/epjconf/202023000004epjconf_fismat2019_00004Non thermal effects of radiofrequency electromagnetic field exposure on neural cellsGrasso Rosaria0Pellitteri Rosalia1Caravella Santi Armando2Musumeci FrancescoRaciti Giuseppina3Scordino AgataSposito Giovanni4Triglia Antonio5Campisi Agata6University of Catania, Department of Physics and Astronomy “Ettore Majorana”Italian National Research Council, Institute for Biomedical Research and InnovationTemix Engineering s.r.l.University of Catania Department of Drug Sciences, Section of BiochemistryUniversity of Catania Department of Drug Sciences, Section of BiochemistryUniversity of Catania, Department of Physics and Astronomy “Ettore Majorana”University of Catania Department of Drug Sciences, Section of BiochemistryThe non-thermal mechanisms, underlying the damage induced on human cells by radiofrequency electromagnetic fields (RF-EMFs), are still unclear and only few studies reported about the effect of RFEMFs on self-renewal of neural progenitor cells. In this research, we investigated the influence of low-intensity RF-EMFs on Olfactory Ensheathing Cell (OEC) cultures, typical glia cells showing characteristics of stem cells. Cell cultures were exposed, in far-field condition, at 900 MHz continuous and amplitude modulated EMFs for 10, 15 and 20 min at 37°C. The expression of OEC marker (S-100), stem cell marker (Nestin), cytoskeletal proteins (GFAP and Vimentin), apoptotic pathway activation by Caspase-3 cleavage and cell viability, were evaluated. Surprisingly 20 min of exposure to continuous or amplitude modulated 900 MHz EMF induced a different and significant decrease in cell viability, some dynamic changes in the expression of the analysed markers and in the activation of the apoptotic pathway.https://www.epj-conferences.org/articles/epjconf/pdf/2020/06/epjconf_fismat2019_00004.pdf
collection DOAJ
language English
format Article
sources DOAJ
author Grasso Rosaria
Pellitteri Rosalia
Caravella Santi Armando
Musumeci Francesco
Raciti Giuseppina
Scordino Agata
Sposito Giovanni
Triglia Antonio
Campisi Agata
spellingShingle Grasso Rosaria
Pellitteri Rosalia
Caravella Santi Armando
Musumeci Francesco
Raciti Giuseppina
Scordino Agata
Sposito Giovanni
Triglia Antonio
Campisi Agata
Non thermal effects of radiofrequency electromagnetic field exposure on neural cells
EPJ Web of Conferences
author_facet Grasso Rosaria
Pellitteri Rosalia
Caravella Santi Armando
Musumeci Francesco
Raciti Giuseppina
Scordino Agata
Sposito Giovanni
Triglia Antonio
Campisi Agata
author_sort Grasso Rosaria
title Non thermal effects of radiofrequency electromagnetic field exposure on neural cells
title_short Non thermal effects of radiofrequency electromagnetic field exposure on neural cells
title_full Non thermal effects of radiofrequency electromagnetic field exposure on neural cells
title_fullStr Non thermal effects of radiofrequency electromagnetic field exposure on neural cells
title_full_unstemmed Non thermal effects of radiofrequency electromagnetic field exposure on neural cells
title_sort non thermal effects of radiofrequency electromagnetic field exposure on neural cells
publisher EDP Sciences
series EPJ Web of Conferences
issn 2100-014X
publishDate 2020-01-01
description The non-thermal mechanisms, underlying the damage induced on human cells by radiofrequency electromagnetic fields (RF-EMFs), are still unclear and only few studies reported about the effect of RFEMFs on self-renewal of neural progenitor cells. In this research, we investigated the influence of low-intensity RF-EMFs on Olfactory Ensheathing Cell (OEC) cultures, typical glia cells showing characteristics of stem cells. Cell cultures were exposed, in far-field condition, at 900 MHz continuous and amplitude modulated EMFs for 10, 15 and 20 min at 37°C. The expression of OEC marker (S-100), stem cell marker (Nestin), cytoskeletal proteins (GFAP and Vimentin), apoptotic pathway activation by Caspase-3 cleavage and cell viability, were evaluated. Surprisingly 20 min of exposure to continuous or amplitude modulated 900 MHz EMF induced a different and significant decrease in cell viability, some dynamic changes in the expression of the analysed markers and in the activation of the apoptotic pathway.
url https://www.epj-conferences.org/articles/epjconf/pdf/2020/06/epjconf_fismat2019_00004.pdf
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AT pellitterirosalia nonthermaleffectsofradiofrequencyelectromagneticfieldexposureonneuralcells
AT caravellasantiarmando nonthermaleffectsofradiofrequencyelectromagneticfieldexposureonneuralcells
AT musumecifrancesco nonthermaleffectsofradiofrequencyelectromagneticfieldexposureonneuralcells
AT racitigiuseppina nonthermaleffectsofradiofrequencyelectromagneticfieldexposureonneuralcells
AT scordinoagata nonthermaleffectsofradiofrequencyelectromagneticfieldexposureonneuralcells
AT spositogiovanni nonthermaleffectsofradiofrequencyelectromagneticfieldexposureonneuralcells
AT trigliaantonio nonthermaleffectsofradiofrequencyelectromagneticfieldexposureonneuralcells
AT campisiagata nonthermaleffectsofradiofrequencyelectromagneticfieldexposureonneuralcells
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