15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell
Abstract Background A great number of studies reported that 12/15-lipoxygenase (12/15-LO) played an important role in atherosclerosis. And its arachidonic acid(AA) metabolite, 15(S)-hydroperoxy-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15(S)-HETE), is demonstrated to mediate endothelial dysfunction...
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doaj-ec1e7a1eb96346ab81127cd80e26a61b2020-11-24T21:01:22ZengBMCLipids in Health and Disease1476-511X2017-07-011611910.1186/s12944-017-0518-215-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cellGuohua Ma0Bing Pan1Sufen Ren2Caixia Guo3Yansong Guo4Lixin Wei5Lemin Zheng6Buxing Chen7Department of Cardiology, Beijing Tian Tan Hospital, Capital Medical UniversityThe Institute of Cardiovascular Sciences and Institute of Systems Biomedicine, School of Basic Medical Sciences, and Key Laboratory of Molecular Cardiovascular Sciences of Ministry of Education, Peking University Health Science CenterDepartment of Cardiology, Beijing Tian Tan Hospital, Capital Medical UniversityDepartment of Cardiology, Beijing Tian Tan Hospital, Capital Medical UniversityDepartment of Cardiovascular Medicine, Fujian Provincial HospitalDepartment of Nephrology, Union Hospital, Fujian Medical University Union HospitalThe Institute of Cardiovascular Sciences and Institute of Systems Biomedicine, School of Basic Medical Sciences, and Key Laboratory of Molecular Cardiovascular Sciences of Ministry of Education, Peking University Health Science CenterDepartment of Cardiology, Beijing Tian Tan Hospital, Capital Medical UniversityAbstract Background A great number of studies reported that 12/15-lipoxygenase (12/15-LO) played an important role in atherosclerosis. And its arachidonic acid(AA) metabolite, 15(S)-hydroperoxy-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15(S)-HETE), is demonstrated to mediate endothelial dysfunction. 15-oxo-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15-oxo-ETE) was formed from 15-hydroxyprostaglandin dehydrogenase (PGDH)-mediated oxidation of 15(S)-HETE. However, relatively little is known about the biological effects of 15-oxo-ETE in cardiovascular disease. Here, we explore the likely role of 15-lipoxygenase (LO)-1-mediated AA metabolism,15-oxo-ETE, in the early pathogenesis of atherosclerosis. Methods The 15-oxo-ETE level in serum was detected by means of liquid chromatography and online tandem mass spectrometry (LC-MS/MS). And the underlying mechanisms were illuminated by molecular techniques, including immunoblotting, MTT assay, immunocytochemistry and Immunohistochemistry. Results Increased 15-oxo-ETE level is found in in patients with acute myocardial infarction (AMI). After 15-oxo-ETE treatment, Human umbilical vein endothelial cells (HUVECs) showed more attractive to monocytes, whereas monocyte adhesion is suppressed when treated with PKC inhibitor. In ex vivo study, exposure of arteries from C57 mice and ApoE−/−mice to 15-oxo-ETE led to significantly increased E-selectin expression and monocyte adhesion. Conclusions This is the first report that 15-oxo-ETE promotes early pathological process of atherosclerosis by accelerating E-selectin expression and monocyte adhesion. 15-oxo-ETE -induced monocyte adhesion is partly attributable to activation of PKC.http://link.springer.com/article/10.1186/s12944-017-0518-215-oxo-ETEmonocyte adhesionE-selectinAtherosclerosisPKC |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guohua Ma Bing Pan Sufen Ren Caixia Guo Yansong Guo Lixin Wei Lemin Zheng Buxing Chen |
spellingShingle |
Guohua Ma Bing Pan Sufen Ren Caixia Guo Yansong Guo Lixin Wei Lemin Zheng Buxing Chen 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell Lipids in Health and Disease 15-oxo-ETE monocyte adhesion E-selectin Atherosclerosis PKC |
author_facet |
Guohua Ma Bing Pan Sufen Ren Caixia Guo Yansong Guo Lixin Wei Lemin Zheng Buxing Chen |
author_sort |
Guohua Ma |
title |
15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell |
title_short |
15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell |
title_full |
15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell |
title_fullStr |
15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell |
title_full_unstemmed |
15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell |
title_sort |
15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell |
publisher |
BMC |
series |
Lipids in Health and Disease |
issn |
1476-511X |
publishDate |
2017-07-01 |
description |
Abstract Background A great number of studies reported that 12/15-lipoxygenase (12/15-LO) played an important role in atherosclerosis. And its arachidonic acid(AA) metabolite, 15(S)-hydroperoxy-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15(S)-HETE), is demonstrated to mediate endothelial dysfunction. 15-oxo-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15-oxo-ETE) was formed from 15-hydroxyprostaglandin dehydrogenase (PGDH)-mediated oxidation of 15(S)-HETE. However, relatively little is known about the biological effects of 15-oxo-ETE in cardiovascular disease. Here, we explore the likely role of 15-lipoxygenase (LO)-1-mediated AA metabolism,15-oxo-ETE, in the early pathogenesis of atherosclerosis. Methods The 15-oxo-ETE level in serum was detected by means of liquid chromatography and online tandem mass spectrometry (LC-MS/MS). And the underlying mechanisms were illuminated by molecular techniques, including immunoblotting, MTT assay, immunocytochemistry and Immunohistochemistry. Results Increased 15-oxo-ETE level is found in in patients with acute myocardial infarction (AMI). After 15-oxo-ETE treatment, Human umbilical vein endothelial cells (HUVECs) showed more attractive to monocytes, whereas monocyte adhesion is suppressed when treated with PKC inhibitor. In ex vivo study, exposure of arteries from C57 mice and ApoE−/−mice to 15-oxo-ETE led to significantly increased E-selectin expression and monocyte adhesion. Conclusions This is the first report that 15-oxo-ETE promotes early pathological process of atherosclerosis by accelerating E-selectin expression and monocyte adhesion. 15-oxo-ETE -induced monocyte adhesion is partly attributable to activation of PKC. |
topic |
15-oxo-ETE monocyte adhesion E-selectin Atherosclerosis PKC |
url |
http://link.springer.com/article/10.1186/s12944-017-0518-2 |
work_keys_str_mv |
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