| Summary: | Background and objective: To assess the expression of Nuclear receptor
binding SET domain protein 1 (NSD1) in human gastric cancer tissues and cells and
investigate its possible role in gastric cancer.
Methods: TCGA database was used to assess the expression levels of NSD1
in human gastric cancer tissues. Immunoblot assays were performed to detect NSD1
expression levels in gastric cancer cell lines. MTT and colony formation assays
were conduced to detect its role in the survival of gastric cancer cells. Wound
closure and transwell were performed to investigate the effects of NSD1 on the
motility of gastric cancer cells. Immunoblot assays were also conducted to
confirm its effects on WNT10B/β-catenin pathway.
Results: We found the high expression levels of NSD1 in human
gastric cancer tissues and cell lines. NSD1 depletion suppressed the survival and
motility of gastric cancer cells. Additionally, we revealed NSD1 activated the
WNT10B/β-catenin pathway, therefore promoted gastric cancer progression.
Conclusion: We revealed the high NSD1 expression in gastric cancer
tissues and cells, and thought NSD1 could serve as a promising gastric cancer
target.
|
|---|
