Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy
Immunoimaging scanning probe microscopy was utilized for the low-level detection and quantification of biotinylated G4 poly(amidoamine) PAMAM dendrimers. Results were compared to those of high-performance liquid chromatography (HPLC) and found to provide a vastly improved analytical method for the l...
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Series: | International Journal of Analytical Chemistry |
Online Access: | http://dx.doi.org/10.1155/2012/341260 |
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doaj-ec7770cf049544c69e0662b6f06c16982020-11-24T20:44:14ZengHindawi LimitedInternational Journal of Analytical Chemistry1687-87601687-87792012-01-01201210.1155/2012/341260341260Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe MicroscopyChevelle A. Cason0Thomas A. Fabré1Andrew Buhrlage2Kristi L. Haik3Heather A. Bullen4Department of Chemistry, Northern Kentucky University, Highland Heights, KY 41099, USADepartment of Chemistry, Northern Kentucky University, Highland Heights, KY 41099, USADepartment of Chemistry, Northern Kentucky University, Highland Heights, KY 41099, USADepartment of Biological Sciences, Northern Kentucky University, Highland Heights, KY 41099, USADepartment of Chemistry, Northern Kentucky University, Highland Heights, KY 41099, USAImmunoimaging scanning probe microscopy was utilized for the low-level detection and quantification of biotinylated G4 poly(amidoamine) PAMAM dendrimers. Results were compared to those of high-performance liquid chromatography (HPLC) and found to provide a vastly improved analytical method for the low-level detection of dendrimers, improving the limit of detection by a factor of 1000 (LOD=2.5×10−13 moles). The biorecognition method is reproducible and shows high specificity and good accuracy. In addition, the capture assay platform shows a promising approach to patterning dendrimers for nanotechnology applications.http://dx.doi.org/10.1155/2012/341260 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chevelle A. Cason Thomas A. Fabré Andrew Buhrlage Kristi L. Haik Heather A. Bullen |
spellingShingle |
Chevelle A. Cason Thomas A. Fabré Andrew Buhrlage Kristi L. Haik Heather A. Bullen Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy International Journal of Analytical Chemistry |
author_facet |
Chevelle A. Cason Thomas A. Fabré Andrew Buhrlage Kristi L. Haik Heather A. Bullen |
author_sort |
Chevelle A. Cason |
title |
Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy |
title_short |
Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy |
title_full |
Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy |
title_fullStr |
Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy |
title_full_unstemmed |
Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy |
title_sort |
low-level detection of poly(amidoamine) pamam dendrimers using immunoimaging scanning probe microscopy |
publisher |
Hindawi Limited |
series |
International Journal of Analytical Chemistry |
issn |
1687-8760 1687-8779 |
publishDate |
2012-01-01 |
description |
Immunoimaging scanning probe microscopy was utilized for the low-level detection and quantification of biotinylated G4 poly(amidoamine) PAMAM dendrimers. Results were compared to those of high-performance liquid chromatography (HPLC) and found to provide a vastly improved analytical method for the low-level detection of dendrimers, improving the limit of detection by a factor of 1000 (LOD=2.5×10−13 moles). The biorecognition method is reproducible and shows high specificity and good accuracy. In addition, the capture assay platform shows a promising approach to patterning dendrimers for nanotechnology applications. |
url |
http://dx.doi.org/10.1155/2012/341260 |
work_keys_str_mv |
AT chevelleacason lowleveldetectionofpolyamidoaminepamamdendrimersusingimmunoimagingscanningprobemicroscopy AT thomasafabre lowleveldetectionofpolyamidoaminepamamdendrimersusingimmunoimagingscanningprobemicroscopy AT andrewbuhrlage lowleveldetectionofpolyamidoaminepamamdendrimersusingimmunoimagingscanningprobemicroscopy AT kristilhaik lowleveldetectionofpolyamidoaminepamamdendrimersusingimmunoimagingscanningprobemicroscopy AT heatherabullen lowleveldetectionofpolyamidoaminepamamdendrimersusingimmunoimagingscanningprobemicroscopy |
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1716818080657571840 |