Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy

Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy

Immunoimaging scanning probe microscopy was utilized for the low-level detection and quantification of biotinylated G4 poly(amidoamine) PAMAM dendrimers. Results were compared to those of high-performance liquid chromatography (HPLC) and found to provide a vastly improved analytical method for the l...

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Main Authors: Chevelle A. Cason, Thomas A. Fabré, Andrew Buhrlage, Kristi L. Haik, Heather A. Bullen
Format: Article
Language:English
Published: Hindawi Limited 2012-01-01
Series:International Journal of Analytical Chemistry
Online Access:http://dx.doi.org/10.1155/2012/341260
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spelling doaj-ec7770cf049544c69e0662b6f06c16982020-11-24T20:44:14ZengHindawi LimitedInternational Journal of Analytical Chemistry1687-87601687-87792012-01-01201210.1155/2012/341260341260Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe MicroscopyChevelle A. Cason0Thomas A. Fabré1Andrew Buhrlage2Kristi L. Haik3Heather A. Bullen4Department of Chemistry, Northern Kentucky University, Highland Heights, KY 41099, USADepartment of Chemistry, Northern Kentucky University, Highland Heights, KY 41099, USADepartment of Chemistry, Northern Kentucky University, Highland Heights, KY 41099, USADepartment of Biological Sciences, Northern Kentucky University, Highland Heights, KY 41099, USADepartment of Chemistry, Northern Kentucky University, Highland Heights, KY 41099, USAImmunoimaging scanning probe microscopy was utilized for the low-level detection and quantification of biotinylated G4 poly(amidoamine) PAMAM dendrimers. Results were compared to those of high-performance liquid chromatography (HPLC) and found to provide a vastly improved analytical method for the low-level detection of dendrimers, improving the limit of detection by a factor of 1000 (LOD=2.5×10−13 moles). The biorecognition method is reproducible and shows high specificity and good accuracy. In addition, the capture assay platform shows a promising approach to patterning dendrimers for nanotechnology applications.http://dx.doi.org/10.1155/2012/341260
collection DOAJ
language English
format Article
sources DOAJ
author Chevelle A. Cason
Thomas A. Fabré
Andrew Buhrlage
Kristi L. Haik
Heather A. Bullen
spellingShingle Chevelle A. Cason
Thomas A. Fabré
Andrew Buhrlage
Kristi L. Haik
Heather A. Bullen
Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy
International Journal of Analytical Chemistry
author_facet Chevelle A. Cason
Thomas A. Fabré
Andrew Buhrlage
Kristi L. Haik
Heather A. Bullen
author_sort Chevelle A. Cason
title Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy
title_short Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy
title_full Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy
title_fullStr Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy
title_full_unstemmed Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy
title_sort low-level detection of poly(amidoamine) pamam dendrimers using immunoimaging scanning probe microscopy
publisher Hindawi Limited
series International Journal of Analytical Chemistry
issn 1687-8760
1687-8779
publishDate 2012-01-01
description Immunoimaging scanning probe microscopy was utilized for the low-level detection and quantification of biotinylated G4 poly(amidoamine) PAMAM dendrimers. Results were compared to those of high-performance liquid chromatography (HPLC) and found to provide a vastly improved analytical method for the low-level detection of dendrimers, improving the limit of detection by a factor of 1000 (LOD=2.5×10−13 moles). The biorecognition method is reproducible and shows high specificity and good accuracy. In addition, the capture assay platform shows a promising approach to patterning dendrimers for nanotechnology applications.
url http://dx.doi.org/10.1155/2012/341260
work_keys_str_mv AT chevelleacason lowleveldetectionofpolyamidoaminepamamdendrimersusingimmunoimagingscanningprobemicroscopy
AT thomasafabre lowleveldetectionofpolyamidoaminepamamdendrimersusingimmunoimagingscanningprobemicroscopy
AT andrewbuhrlage lowleveldetectionofpolyamidoaminepamamdendrimersusingimmunoimagingscanningprobemicroscopy
AT kristilhaik lowleveldetectionofpolyamidoaminepamamdendrimersusingimmunoimagingscanningprobemicroscopy
AT heatherabullen lowleveldetectionofpolyamidoaminepamamdendrimersusingimmunoimagingscanningprobemicroscopy
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