Adiponectin protein exists in aortic endothelial cells.
AIMS: Inflammation is closely associated with the development of atherosclerosis and metabolic syndrome. Adiponectin, an adipose-derived secretory protein, possesses an anti-atherosclerotic property. The present study was undertaken to elucidate the presence and significance of adiponectin in vascul...
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doaj-ec8a8c467a8649dd8a3e20234956cd2a2020-11-24T21:12:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7127110.1371/journal.pone.0071271Adiponectin protein exists in aortic endothelial cells.Noriyuki KomuraNorikazu MaedaTakuya MoriShinji KiharaHideaki NakatsujiAyumu HirataYoshihiro TochinoTohru FunahashiIichiro ShimomuraAIMS: Inflammation is closely associated with the development of atherosclerosis and metabolic syndrome. Adiponectin, an adipose-derived secretory protein, possesses an anti-atherosclerotic property. The present study was undertaken to elucidate the presence and significance of adiponectin in vasculature. METHODS AND RESULTS: Immunofluorescence staining was performed in aorta of wild-type (WT) mice and demonstrated that adiponectin was co-stained with CD31. Thoracic aorta was cut through and then aortic intima was carefully shaved from aorta. Western blotting showed the existence of adiponectin protein in aortic intima, while there was no adiponectin mRNA expression. Adiponectin knockout (Adipo-KO) and WT mice were administered with a low-dose and short-term lipopolysaccharide (LPS) (1 mg/kg of LPS for 4 hours). The endothelium vascular adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) were highly increased in Adipo-KO mice compared to WT mice after LPS administration. CONCLUSIONS: Adiponectin protein exists in aortic endothelium under steady state and may protect vasculature from the initiation of atherosclerosis.http://europepmc.org/articles/PMC3742782?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Noriyuki Komura Norikazu Maeda Takuya Mori Shinji Kihara Hideaki Nakatsuji Ayumu Hirata Yoshihiro Tochino Tohru Funahashi Iichiro Shimomura |
spellingShingle |
Noriyuki Komura Norikazu Maeda Takuya Mori Shinji Kihara Hideaki Nakatsuji Ayumu Hirata Yoshihiro Tochino Tohru Funahashi Iichiro Shimomura Adiponectin protein exists in aortic endothelial cells. PLoS ONE |
author_facet |
Noriyuki Komura Norikazu Maeda Takuya Mori Shinji Kihara Hideaki Nakatsuji Ayumu Hirata Yoshihiro Tochino Tohru Funahashi Iichiro Shimomura |
author_sort |
Noriyuki Komura |
title |
Adiponectin protein exists in aortic endothelial cells. |
title_short |
Adiponectin protein exists in aortic endothelial cells. |
title_full |
Adiponectin protein exists in aortic endothelial cells. |
title_fullStr |
Adiponectin protein exists in aortic endothelial cells. |
title_full_unstemmed |
Adiponectin protein exists in aortic endothelial cells. |
title_sort |
adiponectin protein exists in aortic endothelial cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
AIMS: Inflammation is closely associated with the development of atherosclerosis and metabolic syndrome. Adiponectin, an adipose-derived secretory protein, possesses an anti-atherosclerotic property. The present study was undertaken to elucidate the presence and significance of adiponectin in vasculature. METHODS AND RESULTS: Immunofluorescence staining was performed in aorta of wild-type (WT) mice and demonstrated that adiponectin was co-stained with CD31. Thoracic aorta was cut through and then aortic intima was carefully shaved from aorta. Western blotting showed the existence of adiponectin protein in aortic intima, while there was no adiponectin mRNA expression. Adiponectin knockout (Adipo-KO) and WT mice were administered with a low-dose and short-term lipopolysaccharide (LPS) (1 mg/kg of LPS for 4 hours). The endothelium vascular adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) were highly increased in Adipo-KO mice compared to WT mice after LPS administration. CONCLUSIONS: Adiponectin protein exists in aortic endothelium under steady state and may protect vasculature from the initiation of atherosclerosis. |
url |
http://europepmc.org/articles/PMC3742782?pdf=render |
work_keys_str_mv |
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