Variability in Catechin and Rutin Contents and Their Antioxidant Potential in Diverse Apple Genotypes
Catechins and rutin are among the main metabolites found in apple fruit. Sixty apple genotypes, harvested in 2016 and 2017, were analyzed for their phenolic content and antioxidant activity. The HPLC analysis showed that the catechin concentration ranged from 109.98 to 5290.47 µg/g, and th...
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doaj-eca0edd887024b92a805355bfaf407b42020-11-25T00:05:02ZengMDPI AGMolecules1420-30492019-03-0124594310.3390/molecules24050943molecules24050943Variability in Catechin and Rutin Contents and Their Antioxidant Potential in Diverse Apple GenotypesWajida Shafi0Sheikh Mansoor1Sumira Jan2Desh Beer Singh3Mohsin Kazi4Mohammad Raish5Majed Alwadei6Javid Iqbal Mir7Parvaiz Ahmad8Indian Council of Agricultural and Research Central Institute of Temperate Horticulture, Old Airport Road, Rangreth, Srinagar 190007, J&K, IndiaDepartment of Biochemistry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu 180009, J&K, IndiaIndian Council of Agricultural and Research Central Institute of Temperate Horticulture, Old Airport Road, Rangreth, Srinagar 190007, J&K, IndiaIndian Council of Agricultural and Research Central Institute of Temperate Horticulture, Old Airport Road, Rangreth, Srinagar 190007, J&K, IndiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 12372, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 12372, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 12372, Saudi ArabiaIndian Council of Agricultural and Research Central Institute of Temperate Horticulture, Old Airport Road, Rangreth, Srinagar 190007, J&K, IndiaBotany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi ArabiaCatechins and rutin are among the main metabolites found in apple fruit. Sixty apple genotypes, harvested in 2016 and 2017, were analyzed for their phenolic content and antioxidant activity. The HPLC analysis showed that the catechin concentration ranged from 109.98 to 5290.47 µg/g, and the rutin concentration ranged from 12.136 to 483.89 µg/g of apple fruit. The level of DPPH activity ranged from 9.04% to 77.57%, and almost half of the 15 genotypes showed below 30–40% DPPH activity. The apple genotypes ‘Lal Ambri’, ‘Green Sleeves’, and ‘Mallus floribunda’ showed the highest DPPH activity of between 70% and 80%, while ‘Schlomit’, ‘Luxtons Fortune’, ‘Mayaan’, ‘Ananas Retrine’, and ‘Chaubatia ambrose’ showed the lowest ferric reducing antioxidant power (FRAP) activity (0.02–0.09%). Statistical analysis showed a correlation between DPPH activity and catechin content (r = 0.7348) and rutin content (r = 0.1442). Regarding antioxidant activity, fractionated samples of apple genotypes revealed significant activity comparable to that of ascorbic acid. There was also a consistent trend for FRAP activity among all apple genotypes and a significant positive correlation between FRAP activity and rutin content (r = 0.244). Thus, this study reveals a significant variation in antioxidant potential among apple genotypes. This data could be useful for the development of new apple varieties with added phytochemicals by conventional and modern breeders.http://www.mdpi.com/1420-3049/24/5/943appleDPPHFRAPpolyphenolicscatechinsrutin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wajida Shafi Sheikh Mansoor Sumira Jan Desh Beer Singh Mohsin Kazi Mohammad Raish Majed Alwadei Javid Iqbal Mir Parvaiz Ahmad |
spellingShingle |
Wajida Shafi Sheikh Mansoor Sumira Jan Desh Beer Singh Mohsin Kazi Mohammad Raish Majed Alwadei Javid Iqbal Mir Parvaiz Ahmad Variability in Catechin and Rutin Contents and Their Antioxidant Potential in Diverse Apple Genotypes Molecules apple DPPH FRAP polyphenolics catechins rutin |
author_facet |
Wajida Shafi Sheikh Mansoor Sumira Jan Desh Beer Singh Mohsin Kazi Mohammad Raish Majed Alwadei Javid Iqbal Mir Parvaiz Ahmad |
author_sort |
Wajida Shafi |
title |
Variability in Catechin and Rutin Contents and Their Antioxidant Potential in Diverse Apple Genotypes |
title_short |
Variability in Catechin and Rutin Contents and Their Antioxidant Potential in Diverse Apple Genotypes |
title_full |
Variability in Catechin and Rutin Contents and Their Antioxidant Potential in Diverse Apple Genotypes |
title_fullStr |
Variability in Catechin and Rutin Contents and Their Antioxidant Potential in Diverse Apple Genotypes |
title_full_unstemmed |
Variability in Catechin and Rutin Contents and Their Antioxidant Potential in Diverse Apple Genotypes |
title_sort |
variability in catechin and rutin contents and their antioxidant potential in diverse apple genotypes |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-03-01 |
description |
Catechins and rutin are among the main metabolites found in apple fruit. Sixty apple genotypes, harvested in 2016 and 2017, were analyzed for their phenolic content and antioxidant activity. The HPLC analysis showed that the catechin concentration ranged from 109.98 to 5290.47 µg/g, and the rutin concentration ranged from 12.136 to 483.89 µg/g of apple fruit. The level of DPPH activity ranged from 9.04% to 77.57%, and almost half of the 15 genotypes showed below 30–40% DPPH activity. The apple genotypes ‘Lal Ambri’, ‘Green Sleeves’, and ‘Mallus floribunda’ showed the highest DPPH activity of between 70% and 80%, while ‘Schlomit’, ‘Luxtons Fortune’, ‘Mayaan’, ‘Ananas Retrine’, and ‘Chaubatia ambrose’ showed the lowest ferric reducing antioxidant power (FRAP) activity (0.02–0.09%). Statistical analysis showed a correlation between DPPH activity and catechin content (r = 0.7348) and rutin content (r = 0.1442). Regarding antioxidant activity, fractionated samples of apple genotypes revealed significant activity comparable to that of ascorbic acid. There was also a consistent trend for FRAP activity among all apple genotypes and a significant positive correlation between FRAP activity and rutin content (r = 0.244). Thus, this study reveals a significant variation in antioxidant potential among apple genotypes. This data could be useful for the development of new apple varieties with added phytochemicals by conventional and modern breeders. |
topic |
apple DPPH FRAP polyphenolics catechins rutin |
url |
http://www.mdpi.com/1420-3049/24/5/943 |
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