Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair
Tissue engineering (TE) approaches using biomaterials have gain important roles in the regeneration of cartilage. This paper describes the production by microfluidics of alginate-based microfibers containing both extracellular matrix (ECM)-derived biomaterials and chondrocytes. As ECM components gel...
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doaj-eca6e5ef399f4179bcde4f0abde4a5552020-11-25T02:23:48ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852017-06-01510.3389/fbioe.2017.00035264274Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage RepairMarco Angelozzi0Letizia Penolazzi1Stefania Mazzitelli2Elisabetta Lambertini3Andrea Lolli4Roberta Piva5Claudio Nastruzzi6Department of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, ItalyDepartment of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, ItalyDepartment of Life Sciences and Biotechnology, University of Ferrara, Ferrara, ItalyDepartment of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, ItalyDepartment of Orthopaedics, Erasmus MC, University Medical Center, Rotterdam, NetherlandsDepartment of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, ItalyDepartment of Life Sciences and Biotechnology, University of Ferrara, Ferrara, ItalyTissue engineering (TE) approaches using biomaterials have gain important roles in the regeneration of cartilage. This paper describes the production by microfluidics of alginate-based microfibers containing both extracellular matrix (ECM)-derived biomaterials and chondrocytes. As ECM components gelatin or decellularized urinary bladder matrix (UBM) were investigated. The effectiveness of the composite microfibers has been tested to modulate the behavior and redifferentiation of dedifferentiated chondrocytes. The complete redifferentiation, at the single-cell level, of the chondrocytes, without cell aggregate formation, was observed after 14 days of cell culture. Specific chondrogenic markers and high cellular secretory activity was observed in embedded cells. Notably, no sign of collagen type 10 deposition was determined. The obtained data suggest that dedifferentiated chondrocytes regain a functional chondrocyte phenotype when embedded in appropriate 3D scaffold based on alginate plus gelatin or UBM. The proposed scaffolds are indeed valuable to form a cellular microenvironment mimicking the in vivo ECM, opening the way to their use in cartilage TE.http://journal.frontiersin.org/article/10.3389/fbioe.2017.00035/fullmicrofibersextracellular matrix-derived biomaterialschondrocytescartilage tissue engineeringgene expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marco Angelozzi Letizia Penolazzi Stefania Mazzitelli Elisabetta Lambertini Andrea Lolli Roberta Piva Claudio Nastruzzi |
spellingShingle |
Marco Angelozzi Letizia Penolazzi Stefania Mazzitelli Elisabetta Lambertini Andrea Lolli Roberta Piva Claudio Nastruzzi Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair Frontiers in Bioengineering and Biotechnology microfibers extracellular matrix-derived biomaterials chondrocytes cartilage tissue engineering gene expression |
author_facet |
Marco Angelozzi Letizia Penolazzi Stefania Mazzitelli Elisabetta Lambertini Andrea Lolli Roberta Piva Claudio Nastruzzi |
author_sort |
Marco Angelozzi |
title |
Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair |
title_short |
Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair |
title_full |
Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair |
title_fullStr |
Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair |
title_full_unstemmed |
Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair |
title_sort |
dedifferentiated chondrocytes in composite microfibers as tool for cartilage repair |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Bioengineering and Biotechnology |
issn |
2296-4185 |
publishDate |
2017-06-01 |
description |
Tissue engineering (TE) approaches using biomaterials have gain important roles in the regeneration of cartilage. This paper describes the production by microfluidics of alginate-based microfibers containing both extracellular matrix (ECM)-derived biomaterials and chondrocytes. As ECM components gelatin or decellularized urinary bladder matrix (UBM) were investigated. The effectiveness of the composite microfibers has been tested to modulate the behavior and redifferentiation of dedifferentiated chondrocytes. The complete redifferentiation, at the single-cell level, of the chondrocytes, without cell aggregate formation, was observed after 14 days of cell culture. Specific chondrogenic markers and high cellular secretory activity was observed in embedded cells. Notably, no sign of collagen type 10 deposition was determined. The obtained data suggest that dedifferentiated chondrocytes regain a functional chondrocyte phenotype when embedded in appropriate 3D scaffold based on alginate plus gelatin or UBM. The proposed scaffolds are indeed valuable to form a cellular microenvironment mimicking the in vivo ECM, opening the way to their use in cartilage TE. |
topic |
microfibers extracellular matrix-derived biomaterials chondrocytes cartilage tissue engineering gene expression |
url |
http://journal.frontiersin.org/article/10.3389/fbioe.2017.00035/full |
work_keys_str_mv |
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