Metabolomic Salivary Signature of Pediatric Obesity Related Liver Disease and Metabolic Syndrome
Pediatric obesity-related metabolic syndrome (MetS) and nonalcoholic fatty liver disease (NAFLD) are increasingly frequent conditions with a still-elusive diagnosis and low-efficacy treatment and monitoring options. In this study, we investigated the salivary metabolomic signature, which has been un...
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doaj-ecb3c3316ca947fe9d155092bf2e66432020-11-24T21:46:41ZengMDPI AGNutrients2072-66432019-01-0111227410.3390/nu11020274nu11020274Metabolomic Salivary Signature of Pediatric Obesity Related Liver Disease and Metabolic SyndromeJacopo Troisi0Federica Belmonte1Antonella Bisogno2Luca Pierri3Angelo Colucci4Giovanni Scala5Pierpaolo Cavallo6Claudia Mandato7Antonella Di Nuzzi8Laura Di Michele9Anna Pia Delli Bovi10Salvatore Guercio Nuzio11Pietro Vajro12Department of Medicine and Surgery and Dentistry, “Scuola Medica Salernitana”, Pediatrics Section University of Salerno, 84081 Baronissi (Salerno), ItalyDepartment of Medicine and Surgery and Dentistry, “Scuola Medica Salernitana”, Pediatrics Section University of Salerno, 84081 Baronissi (Salerno), ItalyDepartment of Medicine and Surgery and Dentistry, “Scuola Medica Salernitana”, Pediatrics Section University of Salerno, 84081 Baronissi (Salerno), ItalyDepartment of Medicine and Surgery and Dentistry, “Scuola Medica Salernitana”, Pediatrics Section University of Salerno, 84081 Baronissi (Salerno), ItalyDepartment of Medicine and Surgery and Dentistry, “Scuola Medica Salernitana”, Pediatrics Section University of Salerno, 84081 Baronissi (Salerno), ItalyHosmotic srl, Via R. Bosco 178, 80069 Vico Equense (NA), ItalyDepartment of Physics, University of Salerno, 84084 Fisciano (Salerno), ItalyDepartment of Pediatrics, Children’s Hospital Santobono-Pausilipon, 80129 Naples, ItalyDepartment of Medicine and Surgery and Dentistry, “Scuola Medica Salernitana”, Pediatrics Section University of Salerno, 84081 Baronissi (Salerno), ItalyDepartment of Medicine and Surgery and Dentistry, “Scuola Medica Salernitana”, Pediatrics Section University of Salerno, 84081 Baronissi (Salerno), ItalyDepartment of Medicine and Surgery and Dentistry, “Scuola Medica Salernitana”, Pediatrics Section University of Salerno, 84081 Baronissi (Salerno), ItalyDepartment of Medicine and Surgery and Dentistry, “Scuola Medica Salernitana”, Pediatrics Section University of Salerno, 84081 Baronissi (Salerno), ItalyDepartment of Medicine and Surgery and Dentistry, “Scuola Medica Salernitana”, Pediatrics Section University of Salerno, 84081 Baronissi (Salerno), ItalyPediatric obesity-related metabolic syndrome (MetS) and nonalcoholic fatty liver disease (NAFLD) are increasingly frequent conditions with a still-elusive diagnosis and low-efficacy treatment and monitoring options. In this study, we investigated the salivary metabolomic signature, which has been uncharacterized to date. In this pilot-nested case-control study over a transversal design, 41 subjects (23 obese patients and 18 normal weight (NW) healthy controls), characterized based on medical history, clinical, anthropometric, and laboratory data, were recruited. Liver involvement, defined according to ultrasonographic liver brightness, allowed for the allocation of the patients into four groups: obese with hepatic steatosis ([St+], <i>n</i> = 15) and without hepatic steatosis ([St⁻], <i>n</i> = 8), and with (<i>n</i> = 10) and without (<i>n</i> = 13) MetS. A partial least squares discriminant analysis (PLS-DA) model was devised to classify the patients’ classes based on their salivary metabolomic signature. Pediatric obesity and its related liver disease and metabolic syndrome appear to have distinct salivary metabolomic signatures. The difference is notable in metabolites involved in energy, amino and organic acid metabolism, as well as in intestinal bacteria metabolism, possibly reflecting diet, fatty acid synthase pathways, and the strict interaction between microbiota and intestinal mucins. This information expands the current understanding of NAFLD pathogenesis, potentially translating into better targeted monitoring and/or treatment strategies in the future.https://www.mdpi.com/2072-6643/11/2/274pediatric obesitynonalcoholic fatty liver diseasemetabolic syndromesalivametabolomicsgas-chromatography mass spectrometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jacopo Troisi Federica Belmonte Antonella Bisogno Luca Pierri Angelo Colucci Giovanni Scala Pierpaolo Cavallo Claudia Mandato Antonella Di Nuzzi Laura Di Michele Anna Pia Delli Bovi Salvatore Guercio Nuzio Pietro Vajro |
spellingShingle |
Jacopo Troisi Federica Belmonte Antonella Bisogno Luca Pierri Angelo Colucci Giovanni Scala Pierpaolo Cavallo Claudia Mandato Antonella Di Nuzzi Laura Di Michele Anna Pia Delli Bovi Salvatore Guercio Nuzio Pietro Vajro Metabolomic Salivary Signature of Pediatric Obesity Related Liver Disease and Metabolic Syndrome Nutrients pediatric obesity nonalcoholic fatty liver disease metabolic syndrome saliva metabolomics gas-chromatography mass spectrometry |
author_facet |
Jacopo Troisi Federica Belmonte Antonella Bisogno Luca Pierri Angelo Colucci Giovanni Scala Pierpaolo Cavallo Claudia Mandato Antonella Di Nuzzi Laura Di Michele Anna Pia Delli Bovi Salvatore Guercio Nuzio Pietro Vajro |
author_sort |
Jacopo Troisi |
title |
Metabolomic Salivary Signature of Pediatric Obesity Related Liver Disease and Metabolic Syndrome |
title_short |
Metabolomic Salivary Signature of Pediatric Obesity Related Liver Disease and Metabolic Syndrome |
title_full |
Metabolomic Salivary Signature of Pediatric Obesity Related Liver Disease and Metabolic Syndrome |
title_fullStr |
Metabolomic Salivary Signature of Pediatric Obesity Related Liver Disease and Metabolic Syndrome |
title_full_unstemmed |
Metabolomic Salivary Signature of Pediatric Obesity Related Liver Disease and Metabolic Syndrome |
title_sort |
metabolomic salivary signature of pediatric obesity related liver disease and metabolic syndrome |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2019-01-01 |
description |
Pediatric obesity-related metabolic syndrome (MetS) and nonalcoholic fatty liver disease (NAFLD) are increasingly frequent conditions with a still-elusive diagnosis and low-efficacy treatment and monitoring options. In this study, we investigated the salivary metabolomic signature, which has been uncharacterized to date. In this pilot-nested case-control study over a transversal design, 41 subjects (23 obese patients and 18 normal weight (NW) healthy controls), characterized based on medical history, clinical, anthropometric, and laboratory data, were recruited. Liver involvement, defined according to ultrasonographic liver brightness, allowed for the allocation of the patients into four groups: obese with hepatic steatosis ([St+], <i>n</i> = 15) and without hepatic steatosis ([St⁻], <i>n</i> = 8), and with (<i>n</i> = 10) and without (<i>n</i> = 13) MetS. A partial least squares discriminant analysis (PLS-DA) model was devised to classify the patients’ classes based on their salivary metabolomic signature. Pediatric obesity and its related liver disease and metabolic syndrome appear to have distinct salivary metabolomic signatures. The difference is notable in metabolites involved in energy, amino and organic acid metabolism, as well as in intestinal bacteria metabolism, possibly reflecting diet, fatty acid synthase pathways, and the strict interaction between microbiota and intestinal mucins. This information expands the current understanding of NAFLD pathogenesis, potentially translating into better targeted monitoring and/or treatment strategies in the future. |
topic |
pediatric obesity nonalcoholic fatty liver disease metabolic syndrome saliva metabolomics gas-chromatography mass spectrometry |
url |
https://www.mdpi.com/2072-6643/11/2/274 |
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