Genetic Diversity and Prevalence of Giardia duodenalis in Qatar
BackgroundGiardia duodenalis is a common human intestinal parasite worldwide, and the causative agent of diarrhea, with the severity of disease ranging from asymptomatic to intense and debilitating infection. G. duodenalis is known to consist of eight genetically distinct assemblages, named from A t...
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doaj-ecb92d7a21ba4248afc58dbe1699d2f72021-05-04T06:52:07ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-05-011110.3389/fcimb.2021.652946652946Genetic Diversity and Prevalence of Giardia duodenalis in QatarMarwa Chourabi0Sonia Boughattas1Atiyeh M. Abdallah2Atiyeh M. Abdallah3Ahmed Ismail4Jerzy M. Behnke5Hesham M. Al-Mekhlafi6Hesham M. Al-Mekhlafi7Marawan Abu-Madi8Marawan Abu-Madi9Marawan Abu-Madi10Biomedical Research Center, College of Health Sciences-QU Health, Qatar University, Doha, QatarBiomedical Research Center, College of Health Sciences-QU Health, Qatar University, Doha, QatarDepartment of Biomedical Sciences, College of Health Sciences-QU Health, Doha, QatarBiomedical and Pharmaceutical Research Unit-QU Health, Qatar University, Doha, QatarMedical Commission, Ministry of Public Health, Doha, QatarSchool of Life Sciences, University of Nottingham, Nottingham, United KingdomMedical Research Centre, Jazan University, Jazan, Saudi ArabiaDepartment of Parasitology, Faculty of Medicine and Health Sciences, Sana’a University, Sana’a, YemenBiomedical Research Center, College of Health Sciences-QU Health, Qatar University, Doha, QatarDepartment of Biomedical Sciences, College of Health Sciences-QU Health, Doha, QatarBiomedical and Pharmaceutical Research Unit-QU Health, Qatar University, Doha, QatarBackgroundGiardia duodenalis is a common human intestinal parasite worldwide, and the causative agent of diarrhea, with the severity of disease ranging from asymptomatic to intense and debilitating infection. G. duodenalis is known to consist of eight genetically distinct assemblages, named from A to H. No data available on the genotypes and genetic diversity of G. duodenalis circulating in Qatar.MethodsWe genotyped 54 human Giardia isolates, collected from asymptomatic immigrants in Qatar, using a multilocus genotyping (MLGs) tool. We also investigated relationships between the subjects’ genotypes and their demographic data.ResultsGenomic DNA from 54 isolates were tested by PCR and sequence analysis at three loci: glutamate dehydrogenase (gdh), β-giardin (bg) and triose phosphate (tpi)). Assemblage A was identified in nine (16.67%), assemblage B in thirty (55.55%), and a mixture of assemblages A+B in fifteen (27.78%) isolates. All assemblage A isolates, genotyped in different loci, were assigned to sub-assemblage AII, and six of them had MLGs AII-1 while one new MLG was identified in two isolates. Sequences of assemblage B isolates have high level of genetic diversity and high presence of heterogeneous peaks, especially within the gdh gene. No significant associations between genotypes and the immigrants’ demographic data were found due to the extensive number of new variants.ConclusionsMLGs was used herein to genotype 54 immigrant Giardia isolates. The high level of genetic variability found in our isolates hampered MLGs determination, more investigations are now required to consolidate our findings, and to enable a comprehensive understanding of the diversity within G. duodenalis assemblage B isolates.https://www.frontiersin.org/articles/10.3389/fcimb.2021.652946/fullG. duodenalisassemblagesmultilocus genotypingQatarimmigrant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marwa Chourabi Sonia Boughattas Atiyeh M. Abdallah Atiyeh M. Abdallah Ahmed Ismail Jerzy M. Behnke Hesham M. Al-Mekhlafi Hesham M. Al-Mekhlafi Marawan Abu-Madi Marawan Abu-Madi Marawan Abu-Madi |
spellingShingle |
Marwa Chourabi Sonia Boughattas Atiyeh M. Abdallah Atiyeh M. Abdallah Ahmed Ismail Jerzy M. Behnke Hesham M. Al-Mekhlafi Hesham M. Al-Mekhlafi Marawan Abu-Madi Marawan Abu-Madi Marawan Abu-Madi Genetic Diversity and Prevalence of Giardia duodenalis in Qatar Frontiers in Cellular and Infection Microbiology G. duodenalis assemblages multilocus genotyping Qatar immigrant |
author_facet |
Marwa Chourabi Sonia Boughattas Atiyeh M. Abdallah Atiyeh M. Abdallah Ahmed Ismail Jerzy M. Behnke Hesham M. Al-Mekhlafi Hesham M. Al-Mekhlafi Marawan Abu-Madi Marawan Abu-Madi Marawan Abu-Madi |
author_sort |
Marwa Chourabi |
title |
Genetic Diversity and Prevalence of Giardia duodenalis in Qatar |
title_short |
Genetic Diversity and Prevalence of Giardia duodenalis in Qatar |
title_full |
Genetic Diversity and Prevalence of Giardia duodenalis in Qatar |
title_fullStr |
Genetic Diversity and Prevalence of Giardia duodenalis in Qatar |
title_full_unstemmed |
Genetic Diversity and Prevalence of Giardia duodenalis in Qatar |
title_sort |
genetic diversity and prevalence of giardia duodenalis in qatar |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2021-05-01 |
description |
BackgroundGiardia duodenalis is a common human intestinal parasite worldwide, and the causative agent of diarrhea, with the severity of disease ranging from asymptomatic to intense and debilitating infection. G. duodenalis is known to consist of eight genetically distinct assemblages, named from A to H. No data available on the genotypes and genetic diversity of G. duodenalis circulating in Qatar.MethodsWe genotyped 54 human Giardia isolates, collected from asymptomatic immigrants in Qatar, using a multilocus genotyping (MLGs) tool. We also investigated relationships between the subjects’ genotypes and their demographic data.ResultsGenomic DNA from 54 isolates were tested by PCR and sequence analysis at three loci: glutamate dehydrogenase (gdh), β-giardin (bg) and triose phosphate (tpi)). Assemblage A was identified in nine (16.67%), assemblage B in thirty (55.55%), and a mixture of assemblages A+B in fifteen (27.78%) isolates. All assemblage A isolates, genotyped in different loci, were assigned to sub-assemblage AII, and six of them had MLGs AII-1 while one new MLG was identified in two isolates. Sequences of assemblage B isolates have high level of genetic diversity and high presence of heterogeneous peaks, especially within the gdh gene. No significant associations between genotypes and the immigrants’ demographic data were found due to the extensive number of new variants.ConclusionsMLGs was used herein to genotype 54 immigrant Giardia isolates. The high level of genetic variability found in our isolates hampered MLGs determination, more investigations are now required to consolidate our findings, and to enable a comprehensive understanding of the diversity within G. duodenalis assemblage B isolates. |
topic |
G. duodenalis assemblages multilocus genotyping Qatar immigrant |
url |
https://www.frontiersin.org/articles/10.3389/fcimb.2021.652946/full |
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