Generation of one iPSC line (IMEDEAi007-A) by Sendai Virus transduction of PBMCs from a Psoriasis donor

Generation of one iPSC line (IMEDEAi007-A) by Sendai Virus transduction of PBMCs from a Psoriasis donor

Psoriasis is a chronic inflammatory skin disease that speeds up the life cycle of skin cells, forming scales and red patches that are itchy and sometimes painful. It is a complex disease of autoimmune origin and genetic predisposition with more than 10 different loci associated. Here we described th...

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Main Authors: Sara Vallejo-Diez, Aarne Fleischer, José María Martín-Fernández, Almudena Sánchez-Gilabert, Carmelo Gómez-Martínez, Mónica Castresana, Daniel Bachiller
Format: Article
Language:English
Published: Elsevier 2020-08-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S187350612030218X
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spelling doaj-eccafaa7ae024c16a2dd547f6778e36f2020-11-25T02:47:09ZengElsevierStem Cell Research1873-50612020-08-0147101917Generation of one iPSC line (IMEDEAi007-A) by Sendai Virus transduction of PBMCs from a Psoriasis donorSara Vallejo-Diez0Aarne Fleischer1José María Martín-Fernández2Almudena Sánchez-Gilabert3Carmelo Gómez-Martínez4Mónica Castresana5Daniel Bachiller6Consejo Superior de Investigaciones Científicas (CSIC/IMEDEA), Miguel Marqués 21, 07190 Esporles, SpainKARUNA GOOD CELL TECHNOLOGIES SL, C Cercas Bajas 13 Bajo, 01001 Vitoria-Gasteiz, Alava, SpainKARUNA GOOD CELL TECHNOLOGIES SL, C Cercas Bajas 13 Bajo, 01001 Vitoria-Gasteiz, Alava, SpainConsejo Superior de Investigaciones Científicas (CSIC/IMEDEA), Miguel Marqués 21, 07190 Esporles, SpainConsejo Superior de Investigaciones Científicas (CSIC/IMEDEA), Miguel Marqués 21, 07190 Esporles, SpainKARUNA GOOD CELL TECHNOLOGIES SL, C Cercas Bajas 13 Bajo, 01001 Vitoria-Gasteiz, Alava, SpainConsejo Superior de Investigaciones Científicas (CSIC/IMEDEA), Miguel Marqués 21, 07190 Esporles, Spain; Corresponding author.Psoriasis is a chronic inflammatory skin disease that speeds up the life cycle of skin cells, forming scales and red patches that are itchy and sometimes painful. It is a complex disease of autoimmune origin and genetic predisposition with more than 10 different loci associated. Here we described the production of an iPSC line generated by Sendai Virus (Klf4, Oct3/4, Sox2 and c-Myc) reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) from a Psoriasis patient. The iPSC line generated has normal 46XY karyotype, is free of SeV genome and transgenes insertions, express high levels of pluripotency markers and can differentiate into all three germ layers.http://www.sciencedirect.com/science/article/pii/S187350612030218X
collection DOAJ
language English
format Article
sources DOAJ
author Sara Vallejo-Diez
Aarne Fleischer
José María Martín-Fernández
Almudena Sánchez-Gilabert
Carmelo Gómez-Martínez
Mónica Castresana
Daniel Bachiller
spellingShingle Sara Vallejo-Diez
Aarne Fleischer
José María Martín-Fernández
Almudena Sánchez-Gilabert
Carmelo Gómez-Martínez
Mónica Castresana
Daniel Bachiller
Generation of one iPSC line (IMEDEAi007-A) by Sendai Virus transduction of PBMCs from a Psoriasis donor
Stem Cell Research
author_facet Sara Vallejo-Diez
Aarne Fleischer
José María Martín-Fernández
Almudena Sánchez-Gilabert
Carmelo Gómez-Martínez
Mónica Castresana
Daniel Bachiller
author_sort Sara Vallejo-Diez
title Generation of one iPSC line (IMEDEAi007-A) by Sendai Virus transduction of PBMCs from a Psoriasis donor
title_short Generation of one iPSC line (IMEDEAi007-A) by Sendai Virus transduction of PBMCs from a Psoriasis donor
title_full Generation of one iPSC line (IMEDEAi007-A) by Sendai Virus transduction of PBMCs from a Psoriasis donor
title_fullStr Generation of one iPSC line (IMEDEAi007-A) by Sendai Virus transduction of PBMCs from a Psoriasis donor
title_full_unstemmed Generation of one iPSC line (IMEDEAi007-A) by Sendai Virus transduction of PBMCs from a Psoriasis donor
title_sort generation of one ipsc line (imedeai007-a) by sendai virus transduction of pbmcs from a psoriasis donor
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2020-08-01
description Psoriasis is a chronic inflammatory skin disease that speeds up the life cycle of skin cells, forming scales and red patches that are itchy and sometimes painful. It is a complex disease of autoimmune origin and genetic predisposition with more than 10 different loci associated. Here we described the production of an iPSC line generated by Sendai Virus (Klf4, Oct3/4, Sox2 and c-Myc) reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) from a Psoriasis patient. The iPSC line generated has normal 46XY karyotype, is free of SeV genome and transgenes insertions, express high levels of pluripotency markers and can differentiate into all three germ layers.
url http://www.sciencedirect.com/science/article/pii/S187350612030218X
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