Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium
Background. Adhesion molecules are known to influence postoperative organ function, they are hardly involved in the inflammatory response following the ischemia-reperfusion injury. We sought to investigate the potency of small interfering RNAs to suppress adhesion molecule expression in human pulmon...
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Series: | Journal of Nucleic Acids |
Online Access: | http://dx.doi.org/10.4061/2011/694789 |
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doaj-ecd6e3d8ec2447909a8488f928c5a4552020-11-25T00:03:24ZengHindawi LimitedJournal of Nucleic Acids2090-021X2011-01-01201110.4061/2011/694789694789Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular EndotheliumTobias Walker0Julian Siegel1Andrea Nolte2Silke Hartmann3Angela Kornberger4Volker Steger5Hans-Peter Wendel6Department of Thoracic, Cardiac and Vascular Surgery, Tübingen University Hospital, Hoppe-Seyler-Straße 3, 72076 Tübingen, GermanyDepartment of Thoracic, Cardiac and Vascular Surgery, Tübingen University Hospital, Hoppe-Seyler-Straße 3, 72076 Tübingen, GermanyDivision of Congenital and Paediatric Cardiac Surgery, Clinical Research Laboratory, Children's Hospital, Tübingen University Hospital, Hoppe-Seyler-Straße 3, 72076 Tübingen, GermanyDepartment of Anaesthesiology and Intensive Care, Tübingen University Hospital, Hoppe-Seyler-Straße 3, 72076 Tübingen, GermanyDepartment of Thoracic, Cardiac and Vascular Surgery, Tübingen University Hospital, Hoppe-Seyler-Straße 3, 72076 Tübingen, GermanyDepartment of Thoracic, Cardiac and Vascular Surgery, Tübingen University Hospital, Hoppe-Seyler-Straße 3, 72076 Tübingen, GermanyDepartment of Anaesthesiology and Intensive Care, Tübingen University Hospital, Hoppe-Seyler-Straße 3, 72076 Tübingen, GermanyBackground. Adhesion molecules are known to influence postoperative organ function, they are hardly involved in the inflammatory response following the ischemia-reperfusion injury. We sought to investigate the potency of small interfering RNAs to suppress adhesion molecule expression in human pulmonary microvascular endothelial cells. Methods. Human lung microvascular endothelial cells were transfected with specific siRNA followed by a stimulation of the cells with an inflammatory cytokine. Adhesion molecule expression was determined by FACS-analysis, and reduction of intracellular mRNA was determined by qRT-PCR. Furthermore, the attachment of isolated neutrophils on the endothelial layer was determined after siRNA transfection. Results. In summary, siRNA transfection significantly decreased the percentage positive cells in a single cocktail transfection of each adhesion molecule investigated. Adhering neutrophils were diminished as well. Conclusion. siRNA might be a promising tool for the effective suppression of adhesion molecule expression on pulmonary microvascular cells, potentially minimizing leukocyte-endothelial depending interactions of a pulmonary allograft.http://dx.doi.org/10.4061/2011/694789 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tobias Walker Julian Siegel Andrea Nolte Silke Hartmann Angela Kornberger Volker Steger Hans-Peter Wendel |
spellingShingle |
Tobias Walker Julian Siegel Andrea Nolte Silke Hartmann Angela Kornberger Volker Steger Hans-Peter Wendel Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium Journal of Nucleic Acids |
author_facet |
Tobias Walker Julian Siegel Andrea Nolte Silke Hartmann Angela Kornberger Volker Steger Hans-Peter Wendel |
author_sort |
Tobias Walker |
title |
Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium |
title_short |
Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium |
title_full |
Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium |
title_fullStr |
Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium |
title_full_unstemmed |
Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium |
title_sort |
small interfering rna efficiently suppresses adhesion molecule expression on pulmonary microvascular endothelium |
publisher |
Hindawi Limited |
series |
Journal of Nucleic Acids |
issn |
2090-021X |
publishDate |
2011-01-01 |
description |
Background. Adhesion molecules are known to influence postoperative organ function, they are hardly involved in the inflammatory response following the ischemia-reperfusion injury. We sought to investigate the potency of small interfering RNAs to suppress adhesion molecule expression in human pulmonary microvascular endothelial cells. Methods. Human lung microvascular endothelial cells were transfected with specific siRNA followed by a stimulation of the cells with an inflammatory cytokine. Adhesion molecule expression was determined by FACS-analysis, and reduction of intracellular mRNA was determined by qRT-PCR. Furthermore, the attachment of isolated neutrophils on the endothelial layer was determined after siRNA transfection. Results. In summary, siRNA transfection significantly decreased the percentage positive cells in a single cocktail transfection of each adhesion molecule investigated. Adhering neutrophils were diminished as well. Conclusion. siRNA might be a promising tool for the effective suppression of adhesion molecule expression on pulmonary microvascular cells, potentially minimizing leukocyte-endothelial depending interactions of a pulmonary allograft. |
url |
http://dx.doi.org/10.4061/2011/694789 |
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