Screening of microRNAs controlling body fat in Drosophila melanogaster and identification of miR-969 and its target, Gr47b.
MicroRNAs (miRNAs) are small non-protein coding RNAs and post-transcriptionally regulate cellular gene expression. In animal development, miRNAs play essential roles such as stem cell maintenance, organogenesis, and apoptosis. Using gain-of-function (GOF) screening with 160 miRNA lines in Drosophila...
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doaj-ece507dfcfda4a69929c381055a4bb482021-03-03T21:31:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01147e021970710.1371/journal.pone.0219707Screening of microRNAs controlling body fat in Drosophila melanogaster and identification of miR-969 and its target, Gr47b.William RedmondDylan AllenM Christian ElledgeRussell ArellanesLucille RedmondJared YeahquoShuyin ZhangMorgan YoungbloodAustin ReinerJin SeoMicroRNAs (miRNAs) are small non-protein coding RNAs and post-transcriptionally regulate cellular gene expression. In animal development, miRNAs play essential roles such as stem cell maintenance, organogenesis, and apoptosis. Using gain-of-function (GOF) screening with 160 miRNA lines in Drosophila melanogaster, we identified a set of miRNAs which regulates body fat contents and named them microCATs (microRNAs Controlling Adipose Tissue). Further examination of egg-to-adult developmental kinetics of selected miRNA lines showed a negative correlation between fat content and developmental time. Comparison of microCATs with loss-of-function miRNA screening data uncovered miR-969 as an essential regulator of adiposity. Subsequently, we demonstrated adipose tissue-specific knock-down of gustatory receptor 47b (Gr47b), a miR-969 target, greatly reduced the amount of body fat, recapitulating the miR-969 GOF phenotype.https://doi.org/10.1371/journal.pone.0219707 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
William Redmond Dylan Allen M Christian Elledge Russell Arellanes Lucille Redmond Jared Yeahquo Shuyin Zhang Morgan Youngblood Austin Reiner Jin Seo |
spellingShingle |
William Redmond Dylan Allen M Christian Elledge Russell Arellanes Lucille Redmond Jared Yeahquo Shuyin Zhang Morgan Youngblood Austin Reiner Jin Seo Screening of microRNAs controlling body fat in Drosophila melanogaster and identification of miR-969 and its target, Gr47b. PLoS ONE |
author_facet |
William Redmond Dylan Allen M Christian Elledge Russell Arellanes Lucille Redmond Jared Yeahquo Shuyin Zhang Morgan Youngblood Austin Reiner Jin Seo |
author_sort |
William Redmond |
title |
Screening of microRNAs controlling body fat in Drosophila melanogaster and identification of miR-969 and its target, Gr47b. |
title_short |
Screening of microRNAs controlling body fat in Drosophila melanogaster and identification of miR-969 and its target, Gr47b. |
title_full |
Screening of microRNAs controlling body fat in Drosophila melanogaster and identification of miR-969 and its target, Gr47b. |
title_fullStr |
Screening of microRNAs controlling body fat in Drosophila melanogaster and identification of miR-969 and its target, Gr47b. |
title_full_unstemmed |
Screening of microRNAs controlling body fat in Drosophila melanogaster and identification of miR-969 and its target, Gr47b. |
title_sort |
screening of micrornas controlling body fat in drosophila melanogaster and identification of mir-969 and its target, gr47b. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
MicroRNAs (miRNAs) are small non-protein coding RNAs and post-transcriptionally regulate cellular gene expression. In animal development, miRNAs play essential roles such as stem cell maintenance, organogenesis, and apoptosis. Using gain-of-function (GOF) screening with 160 miRNA lines in Drosophila melanogaster, we identified a set of miRNAs which regulates body fat contents and named them microCATs (microRNAs Controlling Adipose Tissue). Further examination of egg-to-adult developmental kinetics of selected miRNA lines showed a negative correlation between fat content and developmental time. Comparison of microCATs with loss-of-function miRNA screening data uncovered miR-969 as an essential regulator of adiposity. Subsequently, we demonstrated adipose tissue-specific knock-down of gustatory receptor 47b (Gr47b), a miR-969 target, greatly reduced the amount of body fat, recapitulating the miR-969 GOF phenotype. |
url |
https://doi.org/10.1371/journal.pone.0219707 |
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