Biosensors and Bio-Bar Code Assays Based on Biofunctionalized Magnetic Microbeads

• Main Authors: Jean-Pierre Cloarec, Yann Chevolot, Claude Martelet, Nicole Jaffrezic-Renault
• Format: Article
• Language: English
• Published: MDPI AG 2007-04-01
• Series: Sensors
• Subjects: Affinity Biosensors; Enzymatic Biosensors; Bio-Bar Code; Magnetic Microbeads
• Online Access: http://www.mdpi.com/1424-8220/7/4/589/

This review paper reports the applications of magnetic microbeads in biosensors and bio-bar code assays. Affinity biosensors are presented through different types of transducing systems: electrochemical, piezo electric or magnetic ones, applied to immunodetection and genodetection. Enzymatic biosensors are based on biofunctionalization through magnetic microbeads of a transducer, more often amperometric, potentiometric or conductimetric. The bio-bar code assays relie on a sandwich structure based on specific biological interaction of a magnetic microbead and a nanoparticle with a defined biological molecule. The magnetic particle allows the separation of the reacted target molecules from unreacted ones. The nanoparticles aim at the amplification and the detection of the target molecule. The bio-bar code assays allow the detection at very low concentration of biological molecules, similar to PCR sensitivity.