Assessment of the Suitability of Methods for Testing the Antioxidant Activity of Anti-Aging Creams
Anti-aging cosmetics are often sought after in order to slow down the aging process. Free radicals are one of the main causes of skin aging, and therefore antioxidants are used in anti-aging cosmetics. The aim of this study was to investigate which method is the most suitable for determining the ant...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-02-01
|
Series: | Applied Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-3417/11/4/1358 |
id |
doaj-ed2d55b94f334289b62b7b51d5e9b442 |
---|---|
record_format |
Article |
spelling |
doaj-ed2d55b94f334289b62b7b51d5e9b4422021-02-04T00:01:34ZengMDPI AGApplied Sciences2076-34172021-02-01111358135810.3390/app11041358Assessment of the Suitability of Methods for Testing the Antioxidant Activity of Anti-Aging CreamsBelma Imamović0Irmela Ivazović1Amra Alispahić2Ervina Bečić3Mirza Dedić4Armina Dacić5Department of Pharmaceutical Analytics, Faculty of Pharmacy, University of Sarajevo, 71000 Sarajevo, Bosnia and HerzegovinaDepartment of Pharmaceutical Analytics, Faculty of Pharmacy, University of Sarajevo, 71000 Sarajevo, Bosnia and HerzegovinaDepartment of Natural Sciences in Pharmacy, Faculty of Pharmacy, University of Sarajevo, 71000 Sarajevo, Bosnia and HerzegovinaDepartment of Pharmaceutical Analytics, Faculty of Pharmacy, University of Sarajevo, 71000 Sarajevo, Bosnia and HerzegovinaDepartment of Pharmaceutical Analytics, Faculty of Pharmacy, University of Sarajevo, 71000 Sarajevo, Bosnia and HerzegovinaDepartment of Pharmaceutical Analytics, Faculty of Pharmacy, University of Sarajevo, 71000 Sarajevo, Bosnia and HerzegovinaAnti-aging cosmetics are often sought after in order to slow down the aging process. Free radicals are one of the main causes of skin aging, and therefore antioxidants are used in anti-aging cosmetics. The aim of this study was to investigate which method is the most suitable for determining the antioxidant capacity of these products. Having samples extracted, the antioxidant capacity of the extracts obtained was determined by the following spectrophotometric methods: DPPH, Folin-Ciocalteu, FRAP, the ABTS method and the ferroion chelation method with ferrosine. The antioxidant capacity of the samples varied depending on the extract type and the method used. DPPH and ferroion chelation measurements with ferrosine were carried out in the part of the spectrum where plant pigments absorb. These pigments are often found in anti-aging products affecting these methods measurement results. The Folin-Ciocalteu method is suitable for researching the antioxidant capacity of hydrophilic extracts, but not lipophilic ones, where turbidity and the formation of a gelled ring occur. The FRAP method revealed similar results for all the samples and proved to be less sensitive than the others. The ABTS method for both types of extracts has proven to be the most suitable and sensitive method for determining the antioxidant capacity of anti-aging products.https://www.mdpi.com/2076-3417/11/4/1358antioxidantsantioxidant capacityanti-aging cosmeticsspectroscopy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Belma Imamović Irmela Ivazović Amra Alispahić Ervina Bečić Mirza Dedić Armina Dacić |
spellingShingle |
Belma Imamović Irmela Ivazović Amra Alispahić Ervina Bečić Mirza Dedić Armina Dacić Assessment of the Suitability of Methods for Testing the Antioxidant Activity of Anti-Aging Creams Applied Sciences antioxidants antioxidant capacity anti-aging cosmetics spectroscopy |
author_facet |
Belma Imamović Irmela Ivazović Amra Alispahić Ervina Bečić Mirza Dedić Armina Dacić |
author_sort |
Belma Imamović |
title |
Assessment of the Suitability of Methods for Testing the Antioxidant Activity of Anti-Aging Creams |
title_short |
Assessment of the Suitability of Methods for Testing the Antioxidant Activity of Anti-Aging Creams |
title_full |
Assessment of the Suitability of Methods for Testing the Antioxidant Activity of Anti-Aging Creams |
title_fullStr |
Assessment of the Suitability of Methods for Testing the Antioxidant Activity of Anti-Aging Creams |
title_full_unstemmed |
Assessment of the Suitability of Methods for Testing the Antioxidant Activity of Anti-Aging Creams |
title_sort |
assessment of the suitability of methods for testing the antioxidant activity of anti-aging creams |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2021-02-01 |
description |
Anti-aging cosmetics are often sought after in order to slow down the aging process. Free radicals are one of the main causes of skin aging, and therefore antioxidants are used in anti-aging cosmetics. The aim of this study was to investigate which method is the most suitable for determining the antioxidant capacity of these products. Having samples extracted, the antioxidant capacity of the extracts obtained was determined by the following spectrophotometric methods: DPPH, Folin-Ciocalteu, FRAP, the ABTS method and the ferroion chelation method with ferrosine. The antioxidant capacity of the samples varied depending on the extract type and the method used. DPPH and ferroion chelation measurements with ferrosine were carried out in the part of the spectrum where plant pigments absorb. These pigments are often found in anti-aging products affecting these methods measurement results. The Folin-Ciocalteu method is suitable for researching the antioxidant capacity of hydrophilic extracts, but not lipophilic ones, where turbidity and the formation of a gelled ring occur. The FRAP method revealed similar results for all the samples and proved to be less sensitive than the others. The ABTS method for both types of extracts has proven to be the most suitable and sensitive method for determining the antioxidant capacity of anti-aging products. |
topic |
antioxidants antioxidant capacity anti-aging cosmetics spectroscopy |
url |
https://www.mdpi.com/2076-3417/11/4/1358 |
work_keys_str_mv |
AT belmaimamovic assessmentofthesuitabilityofmethodsfortestingtheantioxidantactivityofantiagingcreams AT irmelaivazovic assessmentofthesuitabilityofmethodsfortestingtheantioxidantactivityofantiagingcreams AT amraalispahic assessmentofthesuitabilityofmethodsfortestingtheantioxidantactivityofantiagingcreams AT ervinabecic assessmentofthesuitabilityofmethodsfortestingtheantioxidantactivityofantiagingcreams AT mirzadedic assessmentofthesuitabilityofmethodsfortestingtheantioxidantactivityofantiagingcreams AT arminadacic assessmentofthesuitabilityofmethodsfortestingtheantioxidantactivityofantiagingcreams |
_version_ |
1724285989455659008 |