Cyclophilin B facilitates the replication of Orf virus
Abstract Background Viruses interact with host cellular factors to construct a more favourable environment for their efficient replication. Expression of cyclophilin B (CypB), a cellular peptidyl-prolyl cis-trans isomerase (PPIase), was found to be significantly up-regulated. Recently, a number of s...
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doaj-ed317184005542a8aac3e26b31b007692020-11-24T21:55:34ZengBMCVirology Journal1743-422X2017-06-0114111110.1186/s12985-017-0781-xCyclophilin B facilitates the replication of Orf virusKui Zhao0Jida Li1Wenqi He2Deguang Song3Ximu Zhang4Di Zhang5Yanlong Zhou6Feng Gao7College of Veterinary Medicine, Jilin UniversityCollege of Public Hygiene, ZunYi Medical UniversityCollege of Veterinary Medicine, Jilin UniversityCollege of Veterinary Medicine, Jilin UniversityLaboratory Animal Center, Peking UniversityCollege of Veterinary Medicine, Jilin UniversityCollege of Veterinary Medicine, Jilin UniversityCollege of Veterinary Medicine, Jilin UniversityAbstract Background Viruses interact with host cellular factors to construct a more favourable environment for their efficient replication. Expression of cyclophilin B (CypB), a cellular peptidyl-prolyl cis-trans isomerase (PPIase), was found to be significantly up-regulated. Recently, a number of studies have shown that CypB is important in the replication of several viruses, including Japanese encephalitis virus (JEV), hepatitis C virus (HCV) and human papillomavirus type 16 (HPV 16). However, the function of cellular CypB in ORFV replication has not yet been explored. Methods Suppression subtractive hybridization (SSH) technique was applied to identify genes differentially expressed in the ORFV-infected MDBK cells at an early phase of infection. Cellular CypB was confirmed to be significantly up-regulated by quantitative reverse transcription-PCR (qRT-PCR) analysis and Western blotting. The role of CypB in ORFV infection was further determined using Cyclosporin A (CsA) and RNA interference (RNAi). Effect of CypB gene silencing on ORFV replication by 50% tissue culture infectious dose (TCID50) assay and qRT-PCR detection. Results In the present study, CypB was found to be significantly up-regulated in the ORFV-infected MDBK cells at an early phase of infection. Cyclosporin A (CsA) exhibited suppressive effects on ORFV replication through the inhibition of CypB. Silencing of CypB gene inhibited the replication of ORFV in MDBK cells. In conclusion, these data suggest that CypB is critical for the efficient replication of the ORFV genome. Conclusions Cellular CypB was confirmed to be significantly up-regulated in the ORFV-infected MDBK cells at an early phase of infection, which could effectively facilitate the replication of ORFV.http://link.springer.com/article/10.1186/s12985-017-0781-xORFVCellular cyclophilin BCyclosporine ARNA interferenceReplication |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kui Zhao Jida Li Wenqi He Deguang Song Ximu Zhang Di Zhang Yanlong Zhou Feng Gao |
spellingShingle |
Kui Zhao Jida Li Wenqi He Deguang Song Ximu Zhang Di Zhang Yanlong Zhou Feng Gao Cyclophilin B facilitates the replication of Orf virus Virology Journal ORFV Cellular cyclophilin B Cyclosporine A RNA interference Replication |
author_facet |
Kui Zhao Jida Li Wenqi He Deguang Song Ximu Zhang Di Zhang Yanlong Zhou Feng Gao |
author_sort |
Kui Zhao |
title |
Cyclophilin B facilitates the replication of Orf virus |
title_short |
Cyclophilin B facilitates the replication of Orf virus |
title_full |
Cyclophilin B facilitates the replication of Orf virus |
title_fullStr |
Cyclophilin B facilitates the replication of Orf virus |
title_full_unstemmed |
Cyclophilin B facilitates the replication of Orf virus |
title_sort |
cyclophilin b facilitates the replication of orf virus |
publisher |
BMC |
series |
Virology Journal |
issn |
1743-422X |
publishDate |
2017-06-01 |
description |
Abstract Background Viruses interact with host cellular factors to construct a more favourable environment for their efficient replication. Expression of cyclophilin B (CypB), a cellular peptidyl-prolyl cis-trans isomerase (PPIase), was found to be significantly up-regulated. Recently, a number of studies have shown that CypB is important in the replication of several viruses, including Japanese encephalitis virus (JEV), hepatitis C virus (HCV) and human papillomavirus type 16 (HPV 16). However, the function of cellular CypB in ORFV replication has not yet been explored. Methods Suppression subtractive hybridization (SSH) technique was applied to identify genes differentially expressed in the ORFV-infected MDBK cells at an early phase of infection. Cellular CypB was confirmed to be significantly up-regulated by quantitative reverse transcription-PCR (qRT-PCR) analysis and Western blotting. The role of CypB in ORFV infection was further determined using Cyclosporin A (CsA) and RNA interference (RNAi). Effect of CypB gene silencing on ORFV replication by 50% tissue culture infectious dose (TCID50) assay and qRT-PCR detection. Results In the present study, CypB was found to be significantly up-regulated in the ORFV-infected MDBK cells at an early phase of infection. Cyclosporin A (CsA) exhibited suppressive effects on ORFV replication through the inhibition of CypB. Silencing of CypB gene inhibited the replication of ORFV in MDBK cells. In conclusion, these data suggest that CypB is critical for the efficient replication of the ORFV genome. Conclusions Cellular CypB was confirmed to be significantly up-regulated in the ORFV-infected MDBK cells at an early phase of infection, which could effectively facilitate the replication of ORFV. |
topic |
ORFV Cellular cyclophilin B Cyclosporine A RNA interference Replication |
url |
http://link.springer.com/article/10.1186/s12985-017-0781-x |
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