The Current Status and Development of Insect-Resistant Genetically Engineered Poplar in China
Poplar is one of the main afforestation tree species in China, and the use of a single, or only a few, clones with low genetic diversity in poplar plantations has led to increasing problems with insect pests. The use of genetic engineering to cultivate insect-resistant poplar varieties has become a...
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doaj-ed35a6c941cd4d989267fa13cb03bfa92020-11-25T01:04:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-09-01910.3389/fpls.2018.01408328449The Current Status and Development of Insect-Resistant Genetically Engineered Poplar in ChinaGuiying Wang0Guiying Wang1Yan Dong2Yan Dong3Xiaojie Liu4Guosheng Yao5Xiaoyue Yu6Xiaoyue Yu7Minsheng Yang8Minsheng Yang9Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, ChinaLangfang Academy of Agriculture and Forestry Sciences, Langfang, ChinaInstitute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, ChinaHebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, ChinaLangfang Academy of Agriculture and Forestry Sciences, Langfang, ChinaLangfang Academy of Agriculture and Forestry Sciences, Langfang, ChinaInstitute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, ChinaHebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, ChinaInstitute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, ChinaHebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, ChinaPoplar is one of the main afforestation tree species in China, and the use of a single, or only a few, clones with low genetic diversity in poplar plantations has led to increasing problems with insect pests. The use of genetic engineering to cultivate insect-resistant poplar varieties has become a hot topic. Over the past 20 years, there have been remarkable achievements in this area. To date, nearly 22 insect-resistant poplar varieties have been created and approved for small-scale field testing, environmental release, or pilot-scale production. Here, we comprehensively review the development of insect-resistant genetically modified (GM) poplars in China. This review mostly addresses issues surrounding the regulation and commercialization of Bt poplar in China, the various insecticidal genes used, the effects of transgenic poplars on insects, toxic protein expression, multigene transformation, the stability of insect resistance, and biosafety. The efficacy of GM poplars for pest control differed among different transgenic poplar clones, larval instars, and insect species. The Bt protein analysis revealed that the expression level of Cry3A was significantly higher than that of Cry1Ac. Temporal and spatial studies of Bt protein showed that its expression varied with the developmental stage and tissue. The inheritance and expression of the exogenous gene were reviewed in transgenic hybrid poplar progeny lines and grafted sections. Biosafety issues, in terms of transgene stability and the effects on soil microorganisms, natural enemies of insects, and arthropod communities are also discussed.https://www.frontiersin.org/article/10.3389/fpls.2018.01408/fulltransgenic poplarinsect resistance genetoxin protein expressionmultigene transformationbiosafety |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guiying Wang Guiying Wang Yan Dong Yan Dong Xiaojie Liu Guosheng Yao Xiaoyue Yu Xiaoyue Yu Minsheng Yang Minsheng Yang |
spellingShingle |
Guiying Wang Guiying Wang Yan Dong Yan Dong Xiaojie Liu Guosheng Yao Xiaoyue Yu Xiaoyue Yu Minsheng Yang Minsheng Yang The Current Status and Development of Insect-Resistant Genetically Engineered Poplar in China Frontiers in Plant Science transgenic poplar insect resistance gene toxin protein expression multigene transformation biosafety |
author_facet |
Guiying Wang Guiying Wang Yan Dong Yan Dong Xiaojie Liu Guosheng Yao Xiaoyue Yu Xiaoyue Yu Minsheng Yang Minsheng Yang |
author_sort |
Guiying Wang |
title |
The Current Status and Development of Insect-Resistant Genetically Engineered Poplar in China |
title_short |
The Current Status and Development of Insect-Resistant Genetically Engineered Poplar in China |
title_full |
The Current Status and Development of Insect-Resistant Genetically Engineered Poplar in China |
title_fullStr |
The Current Status and Development of Insect-Resistant Genetically Engineered Poplar in China |
title_full_unstemmed |
The Current Status and Development of Insect-Resistant Genetically Engineered Poplar in China |
title_sort |
current status and development of insect-resistant genetically engineered poplar in china |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2018-09-01 |
description |
Poplar is one of the main afforestation tree species in China, and the use of a single, or only a few, clones with low genetic diversity in poplar plantations has led to increasing problems with insect pests. The use of genetic engineering to cultivate insect-resistant poplar varieties has become a hot topic. Over the past 20 years, there have been remarkable achievements in this area. To date, nearly 22 insect-resistant poplar varieties have been created and approved for small-scale field testing, environmental release, or pilot-scale production. Here, we comprehensively review the development of insect-resistant genetically modified (GM) poplars in China. This review mostly addresses issues surrounding the regulation and commercialization of Bt poplar in China, the various insecticidal genes used, the effects of transgenic poplars on insects, toxic protein expression, multigene transformation, the stability of insect resistance, and biosafety. The efficacy of GM poplars for pest control differed among different transgenic poplar clones, larval instars, and insect species. The Bt protein analysis revealed that the expression level of Cry3A was significantly higher than that of Cry1Ac. Temporal and spatial studies of Bt protein showed that its expression varied with the developmental stage and tissue. The inheritance and expression of the exogenous gene were reviewed in transgenic hybrid poplar progeny lines and grafted sections. Biosafety issues, in terms of transgene stability and the effects on soil microorganisms, natural enemies of insects, and arthropod communities are also discussed. |
topic |
transgenic poplar insect resistance gene toxin protein expression multigene transformation biosafety |
url |
https://www.frontiersin.org/article/10.3389/fpls.2018.01408/full |
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