3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet
When administered to rats receiving a high-fat diet (HFD), 3,5-diiodo-L-thyronine (3,5-T2) [at a dose of 25 μg/100 g body weight (BW)] is known to increase energy expenditure and to prevent HFD-induced adiposity. Here, we investigated which cellular and molecular processes in visceral white...
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MDPI AG
2019-01-01
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Online Access: | https://www.mdpi.com/2072-6643/11/2/278 |
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doaj-ed74cf4556e54fb9a226930ff64b4e32 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elena Silvestri Rosalba Senese Federica Cioffi Rita De Matteis Davide Lattanzi Assunta Lombardi Antonia Giacco Anna Maria Salzano Andrea Scaloni Michele Ceccarelli Maria Moreno Fernando Goglia Antonia Lanni Pieter de Lange |
spellingShingle |
Elena Silvestri Rosalba Senese Federica Cioffi Rita De Matteis Davide Lattanzi Assunta Lombardi Antonia Giacco Anna Maria Salzano Andrea Scaloni Michele Ceccarelli Maria Moreno Fernando Goglia Antonia Lanni Pieter de Lange 3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet Nutrients 3,5-diiodo-L-thyronine visceral white adipose tissue ATGL hormone sensitive lipase lipolysis proteomics |
author_facet |
Elena Silvestri Rosalba Senese Federica Cioffi Rita De Matteis Davide Lattanzi Assunta Lombardi Antonia Giacco Anna Maria Salzano Andrea Scaloni Michele Ceccarelli Maria Moreno Fernando Goglia Antonia Lanni Pieter de Lange |
author_sort |
Elena Silvestri |
title |
3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet |
title_short |
3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet |
title_full |
3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet |
title_fullStr |
3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet |
title_full_unstemmed |
3,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat Diet |
title_sort |
3,5-diiodo-l-thyronine exerts metabolically favorable effects on visceral adipose tissue of rats receiving a high-fat diet |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2019-01-01 |
description |
When administered to rats receiving a high-fat diet (HFD), 3,5-diiodo-L-thyronine (3,5-T2) [at a dose of 25 μg/100 g body weight (BW)] is known to increase energy expenditure and to prevent HFD-induced adiposity. Here, we investigated which cellular and molecular processes in visceral white adipose tissue (VAT) contributed to the beneficial effect of 3,5-T2 over time (between 1 day and 4 weeks following administration). 3,5-T2 programmed the adipocyte for lipolysis by rapidly inducing hormone sensitive lipase (HSL) phosphorylation at the protein kinase A-responsive site Ser<sup>563</sup>, accompanied with glycerol release at the 1-week time-point, contributing to the partial normalization of adipocyte volume with respect to control (N) animals. After two weeks, when the adipocyte volumes of HFD-3,5-T2 rats were completely normalized to those of the controls (N), 3,5-T2 consistently induced HSL phosphorylation at Ser<sup>563</sup>, indicative of a combined effect of 3,5-T2-induced adipose lipolysis and increasing non-adipose oxidative metabolism. VAT proteome analysis after 4 weeks of treatment revealed that 3,5-T2 significantly altered the proteomic profile of HFD rats and produced a marked pro-angiogenic action. This was associated with a reduced representation of proteins involved in lipid storage or related to response to oxidative stress, and a normalization of the levels of those involved in lipogenesis-associated mitochondrial function. In conclusion, the prevention of VAT mass-gain by 3,5-T2 occurred through different molecular pathways that, together with the previously reported stimulation of resting metabolism and liver fatty acid oxidation, are associated with an anti adipogenic/lipogenic potential and positively impact on tissue health. |
topic |
3,5-diiodo-L-thyronine visceral white adipose tissue ATGL hormone sensitive lipase lipolysis proteomics |
url |
https://www.mdpi.com/2072-6643/11/2/278 |
work_keys_str_mv |
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doaj-ed74cf4556e54fb9a226930ff64b4e322020-11-25T01:51:07ZengMDPI AGNutrients2072-66432019-01-0111227810.3390/nu11020278nu110202783,5-Diiodo-L-Thyronine Exerts Metabolically Favorable Effects on Visceral Adipose Tissue of Rats Receiving a High-Fat DietElena Silvestri0Rosalba Senese1Federica Cioffi2Rita De Matteis3Davide Lattanzi4Assunta Lombardi5Antonia Giacco6Anna Maria Salzano7Andrea Scaloni8Michele Ceccarelli9Maria Moreno10Fernando Goglia11Antonia Lanni12Pieter de Lange13Dipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, via Port’Arsa 11, 82100 Benevento, ItalyDipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università‘ degli Studi della Campania Luigi Vanvitelli, via Vivaldi 43, 81100 Caserta, ItalyDipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, via Port’Arsa 11, 82100 Benevento, ItalyDip. Scienze Biomolecolari, Salute, Università di Urbino “Carlo Bo”, via Maggetti, 26, 61029 Urbino, ItalyDip. Scienze Biomolecolari, Salute, Università di Urbino “Carlo Bo”, via Maggetti, 26, 61029 Urbino, ItalyDipartimento di Biologia, Università degli Studi di Napoli “Federico II”, via Cinthia, 80126 Napoli, ItalyDipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, via Port’Arsa 11, 82100 Benevento, ItalyProteomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, via Argine, 1085, 80147 Naples, ItalyProteomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, via Argine, 1085, 80147 Naples, ItalyDipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, via Port’Arsa 11, 82100 Benevento, ItalyDipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, via Port’Arsa 11, 82100 Benevento, ItalyDipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, via Port’Arsa 11, 82100 Benevento, ItalyDipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università‘ degli Studi della Campania Luigi Vanvitelli, via Vivaldi 43, 81100 Caserta, ItalyDipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università‘ degli Studi della Campania Luigi Vanvitelli, via Vivaldi 43, 81100 Caserta, ItalyWhen administered to rats receiving a high-fat diet (HFD), 3,5-diiodo-L-thyronine (3,5-T2) [at a dose of 25 μg/100 g body weight (BW)] is known to increase energy expenditure and to prevent HFD-induced adiposity. Here, we investigated which cellular and molecular processes in visceral white adipose tissue (VAT) contributed to the beneficial effect of 3,5-T2 over time (between 1 day and 4 weeks following administration). 3,5-T2 programmed the adipocyte for lipolysis by rapidly inducing hormone sensitive lipase (HSL) phosphorylation at the protein kinase A-responsive site Ser<sup>563</sup>, accompanied with glycerol release at the 1-week time-point, contributing to the partial normalization of adipocyte volume with respect to control (N) animals. After two weeks, when the adipocyte volumes of HFD-3,5-T2 rats were completely normalized to those of the controls (N), 3,5-T2 consistently induced HSL phosphorylation at Ser<sup>563</sup>, indicative of a combined effect of 3,5-T2-induced adipose lipolysis and increasing non-adipose oxidative metabolism. VAT proteome analysis after 4 weeks of treatment revealed that 3,5-T2 significantly altered the proteomic profile of HFD rats and produced a marked pro-angiogenic action. This was associated with a reduced representation of proteins involved in lipid storage or related to response to oxidative stress, and a normalization of the levels of those involved in lipogenesis-associated mitochondrial function. In conclusion, the prevention of VAT mass-gain by 3,5-T2 occurred through different molecular pathways that, together with the previously reported stimulation of resting metabolism and liver fatty acid oxidation, are associated with an anti adipogenic/lipogenic potential and positively impact on tissue health.https://www.mdpi.com/2072-6643/11/2/2783,5-diiodo-L-thyroninevisceral white adipose tissueATGLhormone sensitive lipaselipolysisproteomics |