Hotspots of Malaria Transmission in the Peruvian Amazon: Rapid Assessment through a Parasitological and Serological Survey.
With low and markedly seasonal malaria transmission, increasingly sensitive tools for better stratifying the risk of infection and targeting control interventions are needed. A cross-sectional survey to characterize the current malaria transmission patterns, identify hotspots, and detect recent chan...
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4565712?pdf=render |
id |
doaj-ed967401d35c4f71912bf9b702c36ff6 |
---|---|
record_format |
Article |
spelling |
doaj-ed967401d35c4f71912bf9b702c36ff62020-11-24T21:30:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013745810.1371/journal.pone.0137458Hotspots of Malaria Transmission in the Peruvian Amazon: Rapid Assessment through a Parasitological and Serological Survey.Angel Rosas-AguirreNiko SpeybroeckAlejandro Llanos-CuentasAnna Rosanas-UrgellGabriel Carrasco-EscobarHugo RodriguezDionicia GamboaJuan Contreras-MancillaFreddy AlavaIrene S SoaresEdmond RemarqueUmberto D AlessandroAnnette ErhartWith low and markedly seasonal malaria transmission, increasingly sensitive tools for better stratifying the risk of infection and targeting control interventions are needed. A cross-sectional survey to characterize the current malaria transmission patterns, identify hotspots, and detect recent changes using parasitological and serological measures was conducted in three sites of the Peruvian Amazon.After full census of the study population, 651 participants were interviewed, clinically examined and had a blood sample taken for the detection of malaria parasites (microscopy and PCR) and antibodies against P. vivax (PvMSP119, PvAMA1) and P. falciparum (PfGLURP, PfAMA1) antigens by ELISA. Risk factors for malaria infection (positive PCR) and malaria exposure (seropositivity) were assessed by multivariate survey logistic regression models. Age-specific seroprevalence was analyzed using a reversible catalytic conversion model based on maximum likelihood for generating seroconversion rates (SCR, λ). SaTScan was used to detect spatial clusters of serology-positive individuals within each site.The overall parasite prevalence by PCR was low, i.e. 3.9% for P. vivax and 6.7% for P. falciparum, while the seroprevalence was substantially higher, 33.6% for P. vivax and 22.0% for P. falciparum, with major differences between study sites. Age and location (site) were significantly associated with P. vivax exposure; while location, age and outdoor occupation were associated with P. falciparum exposure. P. falciparum seroprevalence curves showed a stable transmission throughout time, while for P. vivax transmission was better described by a model with two SCRs. The spatial analysis identified well-defined clusters of P. falciparum seropositive individuals in two sites, while it detected only a very small cluster of P. vivax exposure.The use of a single parasitological and serological malaria survey has proven to be an efficient and accurate method to characterize the species specific heterogeneity in malaria transmission at micro-geographical level as well as to identify recent changes in transmission.http://europepmc.org/articles/PMC4565712?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Angel Rosas-Aguirre Niko Speybroeck Alejandro Llanos-Cuentas Anna Rosanas-Urgell Gabriel Carrasco-Escobar Hugo Rodriguez Dionicia Gamboa Juan Contreras-Mancilla Freddy Alava Irene S Soares Edmond Remarque Umberto D Alessandro Annette Erhart |
spellingShingle |
Angel Rosas-Aguirre Niko Speybroeck Alejandro Llanos-Cuentas Anna Rosanas-Urgell Gabriel Carrasco-Escobar Hugo Rodriguez Dionicia Gamboa Juan Contreras-Mancilla Freddy Alava Irene S Soares Edmond Remarque Umberto D Alessandro Annette Erhart Hotspots of Malaria Transmission in the Peruvian Amazon: Rapid Assessment through a Parasitological and Serological Survey. PLoS ONE |
author_facet |
Angel Rosas-Aguirre Niko Speybroeck Alejandro Llanos-Cuentas Anna Rosanas-Urgell Gabriel Carrasco-Escobar Hugo Rodriguez Dionicia Gamboa Juan Contreras-Mancilla Freddy Alava Irene S Soares Edmond Remarque Umberto D Alessandro Annette Erhart |
author_sort |
Angel Rosas-Aguirre |
title |
Hotspots of Malaria Transmission in the Peruvian Amazon: Rapid Assessment through a Parasitological and Serological Survey. |
title_short |
Hotspots of Malaria Transmission in the Peruvian Amazon: Rapid Assessment through a Parasitological and Serological Survey. |
title_full |
Hotspots of Malaria Transmission in the Peruvian Amazon: Rapid Assessment through a Parasitological and Serological Survey. |
title_fullStr |
Hotspots of Malaria Transmission in the Peruvian Amazon: Rapid Assessment through a Parasitological and Serological Survey. |
title_full_unstemmed |
Hotspots of Malaria Transmission in the Peruvian Amazon: Rapid Assessment through a Parasitological and Serological Survey. |
title_sort |
hotspots of malaria transmission in the peruvian amazon: rapid assessment through a parasitological and serological survey. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
With low and markedly seasonal malaria transmission, increasingly sensitive tools for better stratifying the risk of infection and targeting control interventions are needed. A cross-sectional survey to characterize the current malaria transmission patterns, identify hotspots, and detect recent changes using parasitological and serological measures was conducted in three sites of the Peruvian Amazon.After full census of the study population, 651 participants were interviewed, clinically examined and had a blood sample taken for the detection of malaria parasites (microscopy and PCR) and antibodies against P. vivax (PvMSP119, PvAMA1) and P. falciparum (PfGLURP, PfAMA1) antigens by ELISA. Risk factors for malaria infection (positive PCR) and malaria exposure (seropositivity) were assessed by multivariate survey logistic regression models. Age-specific seroprevalence was analyzed using a reversible catalytic conversion model based on maximum likelihood for generating seroconversion rates (SCR, λ). SaTScan was used to detect spatial clusters of serology-positive individuals within each site.The overall parasite prevalence by PCR was low, i.e. 3.9% for P. vivax and 6.7% for P. falciparum, while the seroprevalence was substantially higher, 33.6% for P. vivax and 22.0% for P. falciparum, with major differences between study sites. Age and location (site) were significantly associated with P. vivax exposure; while location, age and outdoor occupation were associated with P. falciparum exposure. P. falciparum seroprevalence curves showed a stable transmission throughout time, while for P. vivax transmission was better described by a model with two SCRs. The spatial analysis identified well-defined clusters of P. falciparum seropositive individuals in two sites, while it detected only a very small cluster of P. vivax exposure.The use of a single parasitological and serological malaria survey has proven to be an efficient and accurate method to characterize the species specific heterogeneity in malaria transmission at micro-geographical level as well as to identify recent changes in transmission. |
url |
http://europepmc.org/articles/PMC4565712?pdf=render |
work_keys_str_mv |
AT angelrosasaguirre hotspotsofmalariatransmissionintheperuvianamazonrapidassessmentthroughaparasitologicalandserologicalsurvey AT nikospeybroeck hotspotsofmalariatransmissionintheperuvianamazonrapidassessmentthroughaparasitologicalandserologicalsurvey AT alejandrollanoscuentas hotspotsofmalariatransmissionintheperuvianamazonrapidassessmentthroughaparasitologicalandserologicalsurvey AT annarosanasurgell hotspotsofmalariatransmissionintheperuvianamazonrapidassessmentthroughaparasitologicalandserologicalsurvey AT gabrielcarrascoescobar hotspotsofmalariatransmissionintheperuvianamazonrapidassessmentthroughaparasitologicalandserologicalsurvey AT hugorodriguez hotspotsofmalariatransmissionintheperuvianamazonrapidassessmentthroughaparasitologicalandserologicalsurvey AT dioniciagamboa hotspotsofmalariatransmissionintheperuvianamazonrapidassessmentthroughaparasitologicalandserologicalsurvey AT juancontrerasmancilla hotspotsofmalariatransmissionintheperuvianamazonrapidassessmentthroughaparasitologicalandserologicalsurvey AT freddyalava hotspotsofmalariatransmissionintheperuvianamazonrapidassessmentthroughaparasitologicalandserologicalsurvey AT irenessoares hotspotsofmalariatransmissionintheperuvianamazonrapidassessmentthroughaparasitologicalandserologicalsurvey AT edmondremarque hotspotsofmalariatransmissionintheperuvianamazonrapidassessmentthroughaparasitologicalandserologicalsurvey AT umbertodalessandro hotspotsofmalariatransmissionintheperuvianamazonrapidassessmentthroughaparasitologicalandserologicalsurvey AT annetteerhart hotspotsofmalariatransmissionintheperuvianamazonrapidassessmentthroughaparasitologicalandserologicalsurvey |
_version_ |
1725963284211826688 |