Cellular proteome datasets of human endothelial cells under physiologic state and after treatment with caffeine and epigallocatechin-3-gallate
Human endothelial cells play several significant roles in vascular biology and homeostasis. We report herein cellular proteome datasets of EA.hy926 human endothelial cells under physiologic condition and after treatment with 100 μM caffeine or EGCG for 24-h. Cellular proteins were extracted and subj...
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doaj-eda5f2400560474abc15da696a1b61c22020-11-24T22:06:22ZengElsevierData in Brief2352-34092019-08-0125Cellular proteome datasets of human endothelial cells under physiologic state and after treatment with caffeine and epigallocatechin-3-gallateChanettee Chanthick0Visith Thongboonkerd1Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandCorresponding author. Medical Proteomics Unit, Office for Research and Development, Siriraj Hospital, Mahidol University, 6th Floor – SiMR Building, 2 Wanglang Road, Bangkoknoi, Bangkok 10700, Thailand.; Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandHuman endothelial cells play several significant roles in vascular biology and homeostasis. We report herein cellular proteome datasets of EA.hy926 human endothelial cells under physiologic condition and after treatment with 100 μM caffeine or EGCG for 24-h. Cellular proteins were extracted and subjected to in-solution tryptic digestion using filter-aided sample preparation (FASP) method. The digested peptides were analyzed by nanoflow liquid chromatography coupled to tandem mass spectrometry (nanoLC-ESI-Qq-TOF MS/MS). Finally, the mass spectral data were searched against the human Swiss-Prot database using Mascot 2.4 search engine and quantified using Skyline v.3.5 software and BiblioSpec algorithm. All of these data were used for further comparative proteomics study followed by bioinformatics analyses to investigate differential biochemical effects of caffeine and EGCG on human endothelial cells (Chanthick et al., 2019) [1]. Keywords: Cellular response, Coffee, Epigallocatechin-3-gallate, Polyphenol, Proteome, Teahttp://www.sciencedirect.com/science/article/pii/S2352340919306468 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chanettee Chanthick Visith Thongboonkerd |
spellingShingle |
Chanettee Chanthick Visith Thongboonkerd Cellular proteome datasets of human endothelial cells under physiologic state and after treatment with caffeine and epigallocatechin-3-gallate Data in Brief |
author_facet |
Chanettee Chanthick Visith Thongboonkerd |
author_sort |
Chanettee Chanthick |
title |
Cellular proteome datasets of human endothelial cells under physiologic state and after treatment with caffeine and epigallocatechin-3-gallate |
title_short |
Cellular proteome datasets of human endothelial cells under physiologic state and after treatment with caffeine and epigallocatechin-3-gallate |
title_full |
Cellular proteome datasets of human endothelial cells under physiologic state and after treatment with caffeine and epigallocatechin-3-gallate |
title_fullStr |
Cellular proteome datasets of human endothelial cells under physiologic state and after treatment with caffeine and epigallocatechin-3-gallate |
title_full_unstemmed |
Cellular proteome datasets of human endothelial cells under physiologic state and after treatment with caffeine and epigallocatechin-3-gallate |
title_sort |
cellular proteome datasets of human endothelial cells under physiologic state and after treatment with caffeine and epigallocatechin-3-gallate |
publisher |
Elsevier |
series |
Data in Brief |
issn |
2352-3409 |
publishDate |
2019-08-01 |
description |
Human endothelial cells play several significant roles in vascular biology and homeostasis. We report herein cellular proteome datasets of EA.hy926 human endothelial cells under physiologic condition and after treatment with 100 μM caffeine or EGCG for 24-h. Cellular proteins were extracted and subjected to in-solution tryptic digestion using filter-aided sample preparation (FASP) method. The digested peptides were analyzed by nanoflow liquid chromatography coupled to tandem mass spectrometry (nanoLC-ESI-Qq-TOF MS/MS). Finally, the mass spectral data were searched against the human Swiss-Prot database using Mascot 2.4 search engine and quantified using Skyline v.3.5 software and BiblioSpec algorithm. All of these data were used for further comparative proteomics study followed by bioinformatics analyses to investigate differential biochemical effects of caffeine and EGCG on human endothelial cells (Chanthick et al., 2019) [1]. Keywords: Cellular response, Coffee, Epigallocatechin-3-gallate, Polyphenol, Proteome, Tea |
url |
http://www.sciencedirect.com/science/article/pii/S2352340919306468 |
work_keys_str_mv |
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