Association Analysis of Agronomic Traits of Foxtail Millet Germplasm using AFLP Marker
<strong>Objective</strong> Considering the importance and application of informative markersin the plant breeding programs, the current research aimed to identify the markers related to morphological traits using the association analysisin foxtail millet genotypes. <strong>Materi...
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Shahid Bahonar University of Kerman
2019-08-01
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doaj-edb67b9802484112a6ee6a2236e0eef22020-11-25T03:18:54ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002019-08-0111217319010.22103/jab.2019.12104.10512393Association Analysis of Agronomic Traits of Foxtail Millet Germplasm using AFLP MarkerAkram Aminizadeh0Ghasem Mohammadineghad1Babak Nakhoda2University of Shahid Bahonar KermanAssociate Professor of Plant Breeding, Faculty of Agriculture & Research and Technology Institute of Plant Production (RTIPP), Shahid-Bahonar University of Kerman, Kerman, IranBabak Nakhoda (PhD) Head, Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Seed and Plant Improvement Campus, Shahid-Fahmideh<strong>Objective</strong> Considering the importance and application of informative markersin the plant breeding programs, the current research aimed to identify the markers related to morphological traits using the association analysisin foxtail millet genotypes. <strong>Materials and methods</strong> The current study attempts to identify molecular markers relevant to morphological traits of 30 foxtail millet (<em>Setaria italica</em> L.) genotypes by applying an association analysis through a Mixed Linear Model (MLM). In order to avoid false linkage, a population structure study was first performed and 9 probable subgroups were observed in the studied genotypes. <strong> </strong> <strong>Results</strong> Association analysis, having taken the structure of the population and kinship relations into consideration in the 30 foxtail millet genotypes in question, represented 38 points of linkage with 12 morphological traits. The coefficient of determination at a highly significant level (P<0.01) was variable from 0.102 and 0.328. The results showed that among the 12 primer combinations of AFLP used in this study, M-CTG/E-AAC, M-CTT/E-AAC and M-CTA/E-AAC were the most efficient in investigating the variety of the genotypes. Also, a number of markers such as M-CAA/E-AAC, M-CTG/E-AGC and M-CTT/E-AAC were connected to several traits such as height, leaf length and width, number of tillers, 1000- seed weight and grain yield, among which the highest connection was with M-CTT/E-AAC. <strong>Conclusion</strong> Identifying joint markers is of paramount importance in plant breeding plans because they make simultaneous selection of several traits possible. These markers can be used in screening germplasms in the presence of close linkages with the controlling genes. They also can be used in QTL identification programs in genotyping of foxtail millet cross populations. Therefore, the findings of this study can be applied in primary selection and breeding plans of foxtail millet using a Marker-Assisted Selection (MAS) process.https://jab.uk.ac.ir/article_2393_26e7a41e21eff7f05dbaf8135d43b584.pdfaflp markerassociation analysisfoxtail millet |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Akram Aminizadeh Ghasem Mohammadineghad Babak Nakhoda |
spellingShingle |
Akram Aminizadeh Ghasem Mohammadineghad Babak Nakhoda Association Analysis of Agronomic Traits of Foxtail Millet Germplasm using AFLP Marker مجله بیوتکنولوژی کشاورزی aflp marker association analysis foxtail millet |
author_facet |
Akram Aminizadeh Ghasem Mohammadineghad Babak Nakhoda |
author_sort |
Akram Aminizadeh |
title |
Association Analysis of Agronomic Traits of Foxtail Millet Germplasm using AFLP Marker |
title_short |
Association Analysis of Agronomic Traits of Foxtail Millet Germplasm using AFLP Marker |
title_full |
Association Analysis of Agronomic Traits of Foxtail Millet Germplasm using AFLP Marker |
title_fullStr |
Association Analysis of Agronomic Traits of Foxtail Millet Germplasm using AFLP Marker |
title_full_unstemmed |
Association Analysis of Agronomic Traits of Foxtail Millet Germplasm using AFLP Marker |
title_sort |
association analysis of agronomic traits of foxtail millet germplasm using aflp marker |
publisher |
Shahid Bahonar University of Kerman |
series |
مجله بیوتکنولوژی کشاورزی |
issn |
2228-6705 2228-6500 |
publishDate |
2019-08-01 |
description |
<strong>Objective</strong>
Considering the importance and application of informative markersin the plant breeding programs, the current research aimed to identify the markers related to morphological traits using the association analysisin foxtail millet genotypes.
<strong>Materials and methods</strong>
The current study attempts to identify molecular markers relevant to morphological traits of 30 foxtail millet (<em>Setaria italica</em> L.) genotypes by applying an association analysis through a Mixed Linear Model (MLM). In order to avoid false linkage, a population structure study was first performed and 9 probable subgroups were observed in the studied genotypes.
<strong> </strong>
<strong>Results</strong>
Association analysis, having taken the structure of the population and kinship relations into consideration in the 30 foxtail millet genotypes in question, represented 38 points of linkage with 12 morphological traits. The coefficient of determination at a highly significant level (P<0.01) was variable from 0.102 and 0.328. The results showed that among the 12 primer combinations of AFLP used in this study, M-CTG/E-AAC, M-CTT/E-AAC and M-CTA/E-AAC were the most efficient in investigating the variety of the genotypes. Also, a number of markers such as M-CAA/E-AAC, M-CTG/E-AGC and M-CTT/E-AAC were connected to several traits such as height, leaf length and width, number of tillers, 1000- seed weight and grain yield, among which the highest connection was with M-CTT/E-AAC.
<strong>Conclusion</strong>
Identifying joint markers is of paramount importance in plant breeding plans because they make simultaneous selection of several traits possible. These markers can be used in screening germplasms in the presence of close linkages with the controlling genes. They also can be used in QTL identification programs in genotyping of foxtail millet cross populations. Therefore, the findings of this study can be applied in primary selection and breeding plans of foxtail millet using a Marker-Assisted Selection (MAS) process. |
topic |
aflp marker association analysis foxtail millet |
url |
https://jab.uk.ac.ir/article_2393_26e7a41e21eff7f05dbaf8135d43b584.pdf |
work_keys_str_mv |
AT akramaminizadeh associationanalysisofagronomictraitsoffoxtailmilletgermplasmusingaflpmarker AT ghasemmohammadineghad associationanalysisofagronomictraitsoffoxtailmilletgermplasmusingaflpmarker AT babaknakhoda associationanalysisofagronomictraitsoffoxtailmilletgermplasmusingaflpmarker |
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