Cellulase production from treated oil palm empty fruit bunch degradation by locally isolated Thermobifida fusca
The aim of this research was to evaluate the production of cellulases from locally isolated bacteria, Thermobifida fusca, using thermal and chemical treated oil palm empty fruit bunch (OPEFB) as substrate in liquid-state fermentation (LSF). T. fusca was successfully isolated and was a dominant cellu...
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North Carolina State University
2013-02-01
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doaj-edb77ef7f96c4b0dbe0668a74582a0472020-11-25T01:11:07ZengNorth Carolina State UniversityBioResources1930-21262013-02-0181676687Cellulase production from treated oil palm empty fruit bunch degradation by locally isolated Thermobifida fuscaM. Nazli NaimEzyana Kamal BahrinMohd Huzairi Mohd ZainudinAzhari Samsu BaharuddinNor Amira Farhana HarunRabitah ZakariaThe aim of this research was to evaluate the production of cellulases from locally isolated bacteria, Thermobifida fusca, using thermal and chemical treated oil palm empty fruit bunch (OPEFB) as substrate in liquid-state fermentation (LSF). T. fusca was successfully isolated and was a dominant cellulase producer in OPEFB composting at the thermophilic stage. Analysis of the surface morphology of OPEFB samples using Scanning Electron Microscopy (SEM) showed that the most significant changes after the combination of thermal and chemical pretreatment was the removal of silica bodies, and this observation was supported by X-ray Diffraction analysis (XRD), Fourier Transform Infrared (FTIR), and Thermogravimetric analysis (TG) showing changes on the hemicelluloses, cellulose, and lignin structures throughout the pretreatment process. As a result of the pretreatment, higher cellulase production by T. fusca was obtained. The highest activity for CMCase, FPase, and β-glucosidase using optimally treated OPEFB were 0.24 U/mL, 0.34 U/mL, and 0.04 U/mL, respectively. Therefore, it can be suggested that the combination of chemical and thermal pretreatments enhances the degradation of OPEFB for subsequent use as fermentation substrate, contributing to a higher cellulases yield by T. fusca.http://www.ncsu.edu/bioresources/BioRes_08/BioRes_08_1_0676_FarhanaHarun_SZNZB_Cellulase_Prodn_OPEFB_Thermobifida_3236.pdfCellulasesThermobifida fuscaPhysicochemical characteristicsOil palm empty fruit bunch |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M. Nazli Naim Ezyana Kamal Bahrin Mohd Huzairi Mohd Zainudin Azhari Samsu Baharuddin Nor Amira Farhana Harun Rabitah Zakaria |
spellingShingle |
M. Nazli Naim Ezyana Kamal Bahrin Mohd Huzairi Mohd Zainudin Azhari Samsu Baharuddin Nor Amira Farhana Harun Rabitah Zakaria Cellulase production from treated oil palm empty fruit bunch degradation by locally isolated Thermobifida fusca BioResources Cellulases Thermobifida fusca Physicochemical characteristics Oil palm empty fruit bunch |
author_facet |
M. Nazli Naim Ezyana Kamal Bahrin Mohd Huzairi Mohd Zainudin Azhari Samsu Baharuddin Nor Amira Farhana Harun Rabitah Zakaria |
author_sort |
M. Nazli Naim |
title |
Cellulase production from treated oil palm empty fruit bunch degradation by locally isolated Thermobifida fusca |
title_short |
Cellulase production from treated oil palm empty fruit bunch degradation by locally isolated Thermobifida fusca |
title_full |
Cellulase production from treated oil palm empty fruit bunch degradation by locally isolated Thermobifida fusca |
title_fullStr |
Cellulase production from treated oil palm empty fruit bunch degradation by locally isolated Thermobifida fusca |
title_full_unstemmed |
Cellulase production from treated oil palm empty fruit bunch degradation by locally isolated Thermobifida fusca |
title_sort |
cellulase production from treated oil palm empty fruit bunch degradation by locally isolated thermobifida fusca |
publisher |
North Carolina State University |
series |
BioResources |
issn |
1930-2126 |
publishDate |
2013-02-01 |
description |
The aim of this research was to evaluate the production of cellulases from locally isolated bacteria, Thermobifida fusca, using thermal and chemical treated oil palm empty fruit bunch (OPEFB) as substrate in liquid-state fermentation (LSF). T. fusca was successfully isolated and was a dominant cellulase producer in OPEFB composting at the thermophilic stage. Analysis of the surface morphology of OPEFB samples using Scanning Electron Microscopy (SEM) showed that the most significant changes after the combination of thermal and chemical pretreatment was the removal of silica bodies, and this observation was supported by X-ray Diffraction analysis (XRD), Fourier Transform Infrared (FTIR), and Thermogravimetric analysis (TG) showing changes on the hemicelluloses, cellulose, and lignin structures throughout the pretreatment process. As a result of the pretreatment, higher cellulase production by T. fusca was obtained. The highest activity for CMCase, FPase, and β-glucosidase using optimally treated OPEFB were 0.24 U/mL, 0.34 U/mL, and 0.04 U/mL, respectively. Therefore, it can be suggested that the combination of chemical and thermal pretreatments enhances the degradation of OPEFB for subsequent use as fermentation substrate, contributing to a higher cellulases yield by T. fusca. |
topic |
Cellulases Thermobifida fusca Physicochemical characteristics Oil palm empty fruit bunch |
url |
http://www.ncsu.edu/bioresources/BioRes_08/BioRes_08_1_0676_FarhanaHarun_SZNZB_Cellulase_Prodn_OPEFB_Thermobifida_3236.pdf |
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